Foxo3 Antibody

Code CSB-PA895304XA01MO
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Product Details

Full Product Name Rabbit anti-Mus musculus (Mouse) Foxo3 Polyclonal antibody
Uniprot No. Q9WVH4
Target Names Foxo3
Alternative Names Foxo3 antibody; Fkhr2 antibody; Foxo3aForkhead box protein O3 antibody
Raised in Rabbit
Species Reactivity Mus musculus (Mouse)
Immunogen Recombinant Mus musculus (Mouse) Foxo3 protein
Immunogen Species Mus musculus (Mouse)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Protein A/G
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB (ensure identification of antigen)
Protocols ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables ① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee ① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time Made-to-order (14-16 weeks)

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Target Background

Function
(From Uniprot)
Transcriptional activator that recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3' and regulates different processes, such as apoptosis and autophagy. Acts as a positive regulator of autophagy in skeletal muscle: in starved cells, enters the nucleus following dephosphorylation and binds the promoters of autophagy genes, such as GABARAP1L, MAP1LC3B and ATG12, thereby activating their expression, resulting in proteolysis of skeletal muscle proteins. Triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation. In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription. Also acts as a key regulator of chondrogenic commitment of skeletal progenitor cells in response to lipid availability: when lipids levels are low, translocates to the nucleus and promotes expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation. Also acts as a key regulator of regulatory T-cells (Treg) differentiation by activating expression of FOXP3.
Gene References into Functions
  1. AMPK stabilizes FOXO3 and suggest a role in the first initiation step of mitochondrial segregation in muscle cells. PMID: 29580989
  2. Data indicate a key role of FoxO3a/Zdhhc3/GluA1 axis in the high-fat diet (HFD)-dependent impairment of cognitive function. PMID: 29222408
  3. Taken together, these data implicate Foxo3 and its transcriptional targets in outer hair cell survival after noise damage. PMID: 28432353
  4. These results reveal mechanisms by which FoxO3a promotes host survival during infection with chronic, virulent intracellular bacteria. PMID: 27599659
  5. findings demonstrate that the mTORC2/AKT/FOXO3a axis plays a critical role in the anti-proliferative and pro-apoptotic effects of lycopene in UVB-induced photocarcinogenesis. PMID: 28585698
  6. The results indicate that increased circulating GH is associated with a reduced ovarian primordial follicle reserve and increased pFoxO3a content in oocytes. PMID: 27771355
  7. Melanoma dormancy in a mouse model is linked to GILZ/FOXO3A-dependent quiescence of disseminated stem-like cells PMID: 27465291
  8. miR-34a might suppress the excessive autophagic activity in alveolar type II epithelial AT-II cells via targeting FoxO3 to reduce the damage of LPS-induced Acute Lung Injury. PMID: 28321785
  9. PPE effectively attenuated oxidative stress and ototoxicity by regulating FoxO3a, and may thus prove to be beneficial in protecting auditory cells from ototoxic drugs. PMID: 28560451
  10. Results show that Foxo3a is depressed in the nucleus while autophagy is impaired, and NLRP3 inflammasome is activated in Kupffer cells (KCs). Over-expression of Foxo3a restores autophagy flux and attenuates activation of the NLRP3 inflammasome via promoting the transcription of Bim. PMID: 28427239
  11. Data indicate that forkhead box O3 (FoxO3) has a central role in the neuronal reprogramming susceptibility of cells, and the importance of FoxO3 appears to change during development. PMID: 27402759
  12. These results suggest that lack of FXR impaired FoxO3a-mediated autophagy and in turn exacerbated alcohol-induced liver injury PMID: 25460735
  13. pro-apoptotic role of miR-34a in PA-induced cholangiocyte lipoapoptosis in culture and in the liver PMID: 28250026
  14. data show that the GSK3B-FOXO3 pathway is activated after partial hepatectomy, and this may be one of the mechanisms that lead to upregulation of hepatic IGF1R after partial hepatectomy. PMID: 28952285
  15. BIM-dependent death during CD8(+) T-cell deletion is FOXO3 independent. PMID: 27323690
  16. MiR-182-5p protects inner ear hair cells from cisplatin-induced apoptosis by inhibiting FOXO3a. PMID: 27607577
  17. Our results show for the first time that DC FOXO3 expression and function is altered in females. In vitro results indicate that these differences may be the result of exposure to estrogen. These differences may be critical considerations for the enhancement of immunotherapy for cancer. PMID: 28229217
  18. Data, including data from studies using transgenic/knockout mice, suggest that FoxO3 activation via post-translational phosphorylation can both induce and maintain autophagic activities in renal tubule epithelium in response to injury from unilateral ureteral obstruction; under these conditions, nuclear expression of FoxO3 is up-regulated in hypoxic proximal tubules exhibiting high levels of autophagy. PMID: 28705935
  19. The Foxo3-Eomes pathway is central to achieve the complete specialized gene program required for pathogenic Th1 cell differentiation. PMID: 27742544
  20. these results show that Cdk5-mediated phospho-regulation of Foxo3 can activate several genes that promote neuronal death and aberrant Abeta processing, thereby contributing to the progression of neurodegenerative pathologies. PMID: 28157684
  21. the transcription factor Forkhead box O3 (FoxO3) was found to be an essential regulator of the maintenance of pluripotency in dormant embryonic stem cells. PMID: 27956699
  22. Loss of Foxo3 function resulted in more severe arthritis in vivo (both clinically and histologically) and was associated with higher titers of anticollagen antibodies and interleukin-6 in the blood. PMID: 27214848
  23. results indicate that DNA damage accrued as a result of elevated ROS in Foxo3(-/-) mutant HSPC is at least partially reversible PMID: 27994057
  24. Regulatory loop between FOXO3 and lipid droplets is required for intestinal inflammation. PMID: 26968210
  25. Maternal diabetes induced caspase 3-dependent apoptosis in Sca1(+) cardiac progenitor cells derived from embryonic day 17.5 (E17.5). Both maternal diabetes and high glucose in vitro activated the pro-apoptotic transcription factor, Forkhead O 3a (FoxO3a) via dephosphorylation at threonine 32 (Thr-32) residue. PMID: 27856257
  26. Mechanistically, exercise-induced nuclear transcription factor FOXO3 binds to the CHCHD4 promoter and represses its expression, preventing the translocation of p53 to the mitochondria and thereby increasing p53 nuclear localization. PMID: 27687729
  27. Kit inactivation within oocytes also led to premature ovarian failure, albeit via a contrasting phenotype. Despite normal initial complements of primordial follicles, oocytes remained dormant with arrested oocyte maturation. Foxo3 protein localization in the nucleus versus cytoplasm explained both mutant phenotypes. PMID: 27500836
  28. Glucocorticoids treatment was found to rescue FOXO3a expression both in SLE mice and in SLE patients. PMID: 27481940
  29. Our results suggest that FoxO3a exerts a negative control over mTOR, and its loss could result in autophagic defects in LD associated with laforin deficiency. PMID: 27107699
  30. Iron-induced skeletal muscle atrophy is suggested to involve the E3 ubiquitin ligase mediated by the reduction of Akt-FOXO3a signaling by oxidative stress. PMID: 27049128
  31. Vesicular stomatitis virus (VSV) infection induced significant up-regulation of miR-223 in murine macrophages. miR-223 directly targets FOXO3 to regulate the type I IFN production. PMID: 27226534
  32. These findings demonstrate that melatonin attenuates cisplatin-induced follicle loss by preventing the phosphorylation of PTEN/AKT/FOXO3a pathway members; thus, melatonin is a potential therapeutic agent for ovarian protection and fertility preservation during chemotherapy in female cancer patients. PMID: 26882203
  33. This work thus demonstrates that S-574 phosphorylation generates a specifically apoptotic form of FOXO3 with decreased transcriptional activity for other well-described FOXO3 functions. PMID: 26470730
  34. SIRT6 up-regulates AMP/ATP and then activates the AMPK-FoxO3alpha axis and further initiates the downstream antioxidant-encoding gene expression, thereby decreasing cellular levels of oxidative stress, and mediating cardioprotection in ischemic heart PMID: 26786260
  35. Kit ligand regulates the subcellular localization of FOXO3 in the neonatal mouse ovary. PMID: 26507072
  36. These data suggest a role for FoxO3a in the maintenance of genome integrity in response to DNA damage that is mediated by H2AX via yet unknown mechanisms. PMID: 26694365
  37. mice. In vitro assays revealed enhanced survival capacity of Myc-driven cells lacking FoxO3, but no change in cell cycling was detected PMID: 26764572
  38. Results point to an antioxidant defense mechanism presented by Sirt3 through the activation of Foxo3a, in microglia PMID: 26523980
  39. Foxo3 circular RNA retards cell cycle progression via forming ternary complexes with p21 and CDK2. PMID: 26861625
  40. Results point to FOXO3 as a potential node that couples mitochondrial metabolism with HSC homeostasis. These findings have critical implications for mechanisms that promote malignant transformation and aging of blood stem and progenitor cells. PMID: 26209246
  41. PXR activation stimulates EGF-mediated hepatocyte proliferation in mice, at least in part, through inhibiting FOXO3 from accelerating cell-cycle progression. PMID: 26574435
  42. FoxO3a is collectively required to maintain MODYrelated gene networks, which in turn are required to enable a gene expression program that permits proper substrate selection (glucose versus fatty acids) for mitochondrial oxidative phosphorylation. PMID: 25264246
  43. Altogether these studies uncover FOXO3 as a novel regulator of erythroblast enucleation and terminal maturation suggesting FOXO3 modulation might be therapeutic in disorders with defective erythroid maturation PMID: 26452208
  44. The life-extending effect of dietary restriction requires Foxo3 in mice. PMID: 25808402
  45. miR-155 regulates the delicate balance between PAK1-mediated proliferation and apoptosis in T cells impacting lymphoid organ size and function. PMID: 26121028
  46. FoxO3 transcription factor is present during both oocyte and embryo in vivo maturation and FoxOs may regulate in vitro embryo development under stress conditions. PMID: 25929834
  47. FOXO3 contributes to the control of neutrophil homeostasis and effector function. PMID: 25969990
  48. The mechanism of Foxo3 in primordial oocyte activation was studied by injection of mRNAs encoding transcription activator-like effector nucleases (TALENs) into mouse zygotes. PMID: 25800339
  49. FoxO3 is a negative regulator of the CD8(+) T-cell response, specifically during the primary expansion. PMID: 25245112
  50. The deletion of Foxo3a in FA mice increased the accumulation of ROS and subsequently de-regulated mitosis and ultimately apoptosis in the NSPCs, leading to hydrocephalus development. PMID: 24483844

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Subcellular Location Cytoplasm, cytosol. Nucleus. Mitochondrion matrix. Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side.
Tissue Specificity Expressed in white and brown adipose tissues (at protein level). Expressed in liver, kidney, lung and colon (at protein level). Expressed in skeletal muscles (at protein level).
Database Links

KEGG: mmu:56484

STRING: 10090.ENSMUSP00000050683

UniGene: Mm.338613

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