24T ELISA Kit Trial Size (Only USD$150/ kit)
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|Intra-assay Precision (Precision within an assay): CV%<8%|
|Three samples of known concentration were tested twenty times on one plate to assess.|
|Inter-assay Precision (Precision between assays): CV%<10%|
|Three samples of known concentration were tested in twenty assays to assess.|
|To assess the linearity of the assay, samples were spiked with high concentrations of human ANX-V in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.|
|The recovery of human ANX-V spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.|
|Sample Type||Average % Recovery||Range|
|EDTA plasma (n=4)||102||95-107|
|These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.|
The ELISA Kit is designed to measure human ANXA5 levels in samples, including serum, plasma, cell culture supernates, or cell lysates. It uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of ANXA5 in samples. The color intensity is measured at 450 nm via a microplate reader.
ANXA5 is known to possess anti-inflammatory and anti-apoptotic properties. It also has a role in cytotoxicity. Due to its preferential phosphatidylserine (PS) binding property, ANXA5 has been used as a marker for the detection of apoptosis-undergoing cells. It regulates cell proliferation, signal transduction, and coagulation. In addition, it has been shown that the binding of ANXA5 to PS+ apoptotic cells can regulate the PS-mediated immunosuppressive clearance, thus enhancing the immunogenicity of apoptotic cells, including irradiated, apoptotic-tumor cells. Also, overexpression of ANXA5 has been detected in many cancers, including renal cell cancer, hepatocellular carcinoma, colorectal cancer, and breast cancer.
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