ANXA5 Antibody

Code CSB-PA001846YA01HU
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate, A549 whole cell lysate
    All lanes: ANXA5 antibody at 1:3000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 36 kDa
    Observed band size: 36 kDa

  • IHC image of CSB-PA001846YA01HU diluted at 1:500 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA001846YA01HU diluted at 1:500 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA001846YA01HU at dilution of 1:100

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) ANXA5 Polyclonal antibody
Uniprot No. P08758
Target Names ANXA5
Alternative Names Anchorin CII antibody; Annexin 5 antibody; Annexin A5 antibody; Annexin V antibody; Annexin-5 antibody; Annexin5 antibody; AnnexinA5 antibody; AnnexinV antibody; ANX 5 antibody; ANX A5 antibody; ANX5 antibody; ANXA5 antibody; ANXA5_HUMAN antibody; Calphobindin I antibody; CBP-I antibody; Endonexin II antibody; ENX 2 antibody; ENX2 antibody; Lipocortin V antibody; PAP I antibody; PAP-I antibody; Placental anticoagulant protein 4 antibody; Placental anticoagulant protein I antibody; PLACENTAL PROTEIN 4 antibody; PP 4 antibody; Pp4 antibody; RPRGL3 antibody; Thromboplastin inhibitor antibody; VAC-alpha antibody; Vascular anticoagulant-alpha antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Annexin A5 protein (2-320AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IHC 1:100-1:1000
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Applications : Line immunoassay (LIA)

Sample type: blood donor serum, bovine serum albumin (BSA)

Review: Reactivity of anti-prothrombin (aPT) mouse monoclonal (a) and anti-annexin V (aAnV) rabbit polyclonal antibodies (b) with phospholipids (PL) and PL-binding proteins by line immunoassay (LIA): aPT and aAnV were run in the LIA alone or together with blood donor serum, bovine serum albumin (BSA) and PT and AnV, respectively.

By Anonymous

Target Background

Function
(From Uniprot)
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Gene References into Functions
  1. AnxA5 2D-network mediates E-cadherin mobility in the plasmalemma that triggers human trophoblasts aggregation and thereby cell fusion. PMID: 28176826
  2. Caspase-3 and -8 and annexin V may serve as diagnostic markers in Ovarian cancer , also explained that the decrement in control of the S phase in the cell cycle may considered one of the significant factors in the development of ovarian tumors PMID: 30197345
  3. When ANXA5 expression increased, cell proliferation was inhibited by regulating the expression of bcl-2 and bax while cell metastasis was suppressed by regulating E-cadherin and MMP-9 expression. PMID: 30010106
  4. Maternal carriers of the ANXA5 M2 haplotype has greater risk of developing placenta-mediated pregnancy complications, such as preeclampsia and intrauterine growth restriction. PMID: 29497952
  5. The domain IV-truncated form of anxA5 is impaired in binding phosphatidylserine liposome and apoptotic cells, and anticoagulation activity. PMID: 29257055
  6. increase in VEGF and Apelin, and decrease in Annexin A5 supports roles of hemo-dynamic alterations in fetoplacental circulation and structural alterations in uteroplacental bed occurring in preeclampsia. PMID: 29208172
  7. In patients with colon cancer, annexin A5 expression in cancer tissues is related to lymph node metastasis and tumor grade. Serum level of annexin A5 is related to annexin A5 expression in cancer tissues. PMID: 29093625
  8. annexin A5 anticoagulant has a role in adverse outcomes in antiphospholipid antibody positive patients with thrombosis or pregnancy complications PMID: 28393472
  9. There was no significant difference in circulating ANXA5 levels between subjects of the non-pregnant, RPL and parous groups (median, 0.25 ng/ml vs. 0.21 ng/ml vs. 0.01 ng/ml, p = 0.14) (Fig. 1A ). Similarly, no significant differences in circulating ANXA5 levels were observed across these three clinical subgroups among M2-carriers or non-carriers, respectively. PMID: 28605660
  10. Serum annexin A5 level was not altered in pre-eclampsia. PMID: 28501283
  11. Annexin A5 was proposed to use in detection of apoptosis. associated with the Breast Cancer. Upregulation of the AA5 protein is detected in Pleomorphic Adenoma of the Parotid Gland. PMID: 28497265
  12. Physiological function of ANXA5 as an embryonic anticoagulant that appears deficient in contiguous specter of thrombophilia-related pregnancy complications culminating more frequently in miscarriage in a maternal M2 carrier background. PMID: 28900802
  13. This study confirmed the proposed role of M2/ANXA5 as embryonic, genetically associated thrombophilia predisposition factor for early recurrent pregnancy loss among ethnic Malay of Malaysia. PMID: 28108842
  14. Circulating anxA5 levels are associated with carotid intima-media thickness but not coronary plaque composition in high-risk patients with diabetes mellitus. PMID: 28592134
  15. Results showed that ANXA5 rs1050606 was significantly associated with left ventricular hypertrophy in Chinese endogenous hypertension patients. PMID: 29095261
  16. Moreover, mechanism study exhibited that Annexin A5 could activate PI3K/Akt/mTOR signaling pathway, promote epithelial-mesenchymal transition (EMT) and the expression of MMP2 and MMP9. Annexin A5 may be a potential prognostic biomarker in RCC and promotes proliferation, migration and invasion of RCC cells via activating PI3K/Akt/mTOR signaling pathway and regulating EMT process and MMP expression. PMID: 28393205
  17. human myotubes rendered deficient for Annexin-A5 (AnxA5) suffer from a severe defect in membrane resealing. PMID: 27286750
  18. This study functionally characterized allelic variants in the ANXA5 promoter, both alone and in combinations, and the results suggest that combinations of several individual variants contribute to modulate the ANXA5 transcriptional activity, most likely through binding of nuclear factors. PMID: 27318245
  19. Chronically increased alveolar annexin V levels, as reflected in increased idiopathic pulmonary fibrosis (IPF) BALF levels, may contribute to the progression of IPF by inducing the release of pro-fibrotic mediators. PMID: 26160872
  20. lower exhaled breath condensate annexin A5 levels in children with exercise-induced asthma compared to non-exercised-induced one PMID: 25796304
  21. The M2 haplotype is a risk factor for early spontaneous abortions, before the 12th week of gestation, and confers about the same relative risk to carriers of both sexes. PMID: 26371709
  22. annexin A5 promoter haplotype M2 may not have a role in recurrent pregnancy loss in Estonia or Denmark PMID: 26135579
  23. Annexin V is fused to an enzyme, creating a fusion protein that converts nontoxic drug precursors, prodrugs, into anticancer compounds while bound to the tumor, therefore mitigating the risk of side effects. PMID: 25899647
  24. Neither the M1/M2 haplotypes nor the tag SNPs in ANXA5 were convincingly associated with pregnancy related venous thromboembolism. PMID: 25495894
  25. Anx5 could protect cardiomyocytes adherens junctions and improve myocardial contractile function via regulation of p120 and anti-inflammation in LPS-induced endotoxemia. PMID: 25799159
  26. The associated male partner risk confirms the proposed role of M2/ANXA5 as a genetic trait impeding embryonic anticoagulation. PMID: 25682309
  27. AnxA5 promotes membrane resealing of injured human trophoblasts. PMID: 25595530
  28. ANXA5 haplotypes/plasma ANXA5 levels were not associated with carotid IMT progression or cardiovascular disease risk in familial hypercholesterolemia patients. PMID: 25525746
  29. The prognostic value of AnV and T cell apoptosis was evaluated in children with nephrotic syndrome in this review. PMID: 25661914
  30. study found neither individual SNPs nor any of the four haplotypes within the ANXA5 gene upstream region were associated with deep venous thrombosis in the Dutch population PMID: 25382354
  31. Lower incidence of M2/ANXA5 carriage in recurrent pregnancy loss patients with elevated lipoprotein(a) levels. PMID: 24335248
  32. Polycystic ovary syndrome subjects showed increased annexin V positive microparticle concentrations compared with healthy volunteers. PMID: 25336711
  33. Annexin A5 inhibits DLBCL cell invasion, MMP-9 expression/activity, and chemoresistance to CHOP through a PI3K-dependent mechanism. PMID: 25323007
  34. Data show involvement of ANXA5 and ILKAP in susceptibility to malignant melanoma PMID: 24743186
  35. annexin A5 promotes GBM cell invasion, MMP-2 expression/activity, and chemoresistance to temozolomide through a PI3K-dependent mechanism. PMID: 25245332
  36. Modified forms of LDL activate human T cells through dendritic cells. Annexin A5 inhibits these effects and promotes induction of regulatory T cells. PMID: 25395618
  37. The annexin 5 in serums of pregnant women and patients with particular types of cancer PMID: 25080795
  38. This review describes the structure, function and genetic expression of ANXA5, as well as its possible implication in recurrent pregnancy loss. PMID: 24152411
  39. We could not prove any association of ANX5 antibodies and systemic lupus erythematosis. PMID: 24872142
  40. genetic association studies in population in Japan: Data suggest that hypomorphic alleles in ANXA5 promoter in placenta (that is, fetal alleles), but not in maternal blood, contributes to onset of pre-eclampsia. PMID: 24140079
  41. This study demonstrated the significant changes in AnxV levels and its function in type 1 diabetic patients. PMID: 24423325
  42. Annexin A5 binds in a cooperative manner to to phosphatidylserine on apoptotic cell membranes. Shrunken apoptotic cells showed the highest Hill coefficient values. PMID: 24304966
  43. frequency of annexin A5 (-1C/T) polymorphism was higher in systemic lupus erythematosus related groups but did not correlate with recurrent pregnancy loss (RPL) or annexin A5 level; anti-annexin A5 IgM level was higher among antiphospholipid syndrome patients and was associated with RPL PMID: 24600987
  44. Annexin-V modified substrate was constructed via layer-by-layer (LBL) method for specific capture of early stage apoptotic Jurkat cells. PMID: 24021657
  45. annexin A5 may play a crucial role in cisplatin-induced toxicity by mediating the mitochondrial apoptotic pathway via the induction and oligomerization of VDAC. PMID: 24318879
  46. Suggest that annezin A5 IgG plays a significant role in producing a hypercoagulable state in primary and secondary antiphospholipid syndrome. PMID: 23981755
  47. Variations within the ANXA5 gene upstream region were confirmed to be risk factors of recurrent pregnancy loss. PMID: 23850300
  48. rs11575945 polymorphism of the annexin-A5 gene is associated with schizophrenia, and its minor allele is responsible for higher levels of the annexin-A5 protein in the blood and represent one of the risk factors for this disease PMID: 24466757
  49. ANXA5 M2 haplotpye appears as an recurrent pregnany loss risk factor in male and female carriers with most distinguishable effects between the 10th and 15th week of gestation. PMID: 23899942
  50. It appears that the haplotype M2/ANXA5 is not associated with the presence of anti-trophoblast antibodies. PMID: 23529182

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Involvement in disease Pregnancy loss, recurrent, 3 (RPRGL3)
Protein Families Annexin family
Database Links

HGNC: 543

OMIM: 131230

KEGG: hsa:308

STRING: 9606.ENSP00000296511

UniGene: Hs.480653

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