ANXA5 Antibody

Datasheet
Code CSB-PA001846YA01HU
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Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Uniprot No. P08758
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate, A549 whole cell lysate
    All lanes: ANXA5 antibody at 1:3000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 36 kDa
    Observed band size: 36 kDa

  • IHC image of CSB-PA001846YA01HU diluted at 1:500 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA001846YA01HU diluted at 1:500 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA001846YA01HU at dilution of 1:100

Immunogen Recombinant Human Annexin A5 protein (2-320AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC; Recommended dilution: WB:1:1000-1:5000, IHC:1:100-1:1000
Relevance This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Annexin A5 (Anchorin CII) (Annexin V) (Annexin-5) (Calphobindin I) (CBP-I) (Endonexin II) (Lipocortin V) (Placental anticoagulant protein 4) (PP4) (Placental anticoagulant protein I) (PAP-I) (Thromboplastin inhibitor) (Vascular anticoagulant-alpha) (VAC-alpha), ANXA5, ANX5 ENX2 PP4
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Research Area Cell Biology
Target Names ANXA5
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
References
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Function This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Involvement in disease Pregnancy loss, recurrent, 3 (RPRGL3)
Protein Families Annexin family
Database Links

HGNC: 543

OMIM: 131230

KEGG: hsa:308

STRING: 9606.ENSP00000296511

UniGene: Hs.480653

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