Human Heat shock protein HSP 90-beta(HSP90AB1) ELISA kit

Code CSB-EL010808HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name heat shock protein 90kDa alpha (cytosolic), class B member 1
Alternative Names 90 kda heat shock protein beta HSP90 beta ELISA Kit; D6S182 ELISA Kit; FLJ26984 ELISA Kit; Heat shock 84 kDa ELISA Kit; Heat shock 90kD protein 1; beta ELISA Kit; Heat shock 90kDa protein 1 beta ELISA Kit; Heat shock protein 90 alpha family class B member 1 ELISA Kit; Heat shock protein 90 kDa ELISA Kit; Heat shock protein 90kDa alpha (cytosolic) class B member 1 ELISA Kit; Heat shock protein 90kDa alpha family class B member 1 ELISA Kit; Heat shock protein beta ELISA Kit; Heat shock protein HSP 90 beta ELISA Kit; Heat shock protein HSP 90-beta ELISA Kit; HS90B_HUMAN ELISA Kit; HSP 84 ELISA Kit; HSP 90 ELISA Kit; HSP 90 b ELISA Kit; HSP 90b ELISA Kit; HSP84 ELISA Kit; HSP90 BETA ELISA Kit; hsp90ab1 ELISA Kit; HSP90B ELISA Kit; HSPC2 ELISA Kit; HSPCB ELISA Kit
Abbreviation HSP90AB1
Uniprot No. P08238
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 ng/mL-100 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human HSP90AB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 96
Range % 91-102
1:2 Average % 104
Range % 99-108
1:4 Average % 92
Range % 85-96
1:8 Average % 98
Range % 95-104
The recovery of human HSP90AB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 93 86-96
EDTA plasma (n=4) 107 103-111
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
100 2.784 2.648 2.716 2.604
50 2.076 2.009 2.043 1.931
25 1.365 1.337 1.351 1.239
12.5 0.777 0.754 0.766 0.654
6.25 0.432 0.416 0.424 0.312
3.12 0.281 0.274 0.278 0.166
1.56 0.196 0.188 0.192 0.080
0 0.113 0.111 0.112  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Target Background

(From Uniprot)
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function
Gene References into Functions
  1. The production of IFN-gamma by T cells stimulated with citrullinated HSP90beta demonstrates a bias toward TH1 immune responses that are likely involved in the pathogenesis of rheumatoid arthritis-interstitial lung disease. PMID: 29968330
  2. the expressions of HSP90AB1 can predict prognosis in astrocytic tumors PMID: 27258564
  3. We found that the nutrient value of the culturing medium and the length of induction had significant effect on Hsp90 production in Escherichia coli. Our fast, single-day purification protocol resulted in a stable, well-folded and pure sample that was resistant to degradation in a reproducible manner. PMID: 28651008
  4. Data show that C allele of rs2282151 was associated with increased expression level of heat shock protein 90 alpha family class B member 1 (HSP90AB1). PMID: 27756247
  5. Hsp90beta induced endothelial cell-dependent tumor angiogenesis by activating VEGFRs transcription. PMID: 28359326
  6. The authors find that the interaction between sB-Raf and the Hsp90 chaperone system is based on contacts with the M domain of Hsp90, which contributes in forming the ternary complex with Cdc37 as long as the kinase is not stabilized by nucleotide. PMID: 27620500
  7. High HSP90B expression is associated with laryngeal carcinoma. PMID: 27959448
  8. The expression level of Hsp90AB1 in lung cancer tissues was significantly higher than that in normal lung tissue and was associated with lung cancer pathological type and overall survival in lung adenocarcinoma patients. PMID: 26903158
  9. Apart from these distinct Cdc37/Hsp90 interfaces, binding of the B-Raf protein kinase to the cochaperone is conserved between mammals and nematodes. PMID: 26511315
  10. HSP90AB1: Helping the good and the bad PMID: 26358502
  11. These results suggest a means by which the hsp90beta interaction could prevent apo-sGCbeta1 from associating with its partner sGCalpha1 subunit while enabling structural changes to assist heme insertion into the H-NOX domain. PMID: 26134567
  12. Casein kinase 2-mediated phosphorylation of Hsp90beta and stabilization of PXR is a key mechanism in the regulation of MDR1 expression. PMID: 25995454
  13. This study identifies overexpression of HSP90 (especially isoform HSP90AB1) and its clients ATR, ATM, and NBS1 as promising markers for radioresistant, aggressive soft tissue sarcomas with particularly poor prognosis. PMID: 26044951
  14. The proteins (HSP90b, TMS1 and L-plastin) in the current study may hold potential in differentiating between melanoma and benign nevi in diagnostically challenging cases. PMID: 25191796
  15. The expression levels of Hsp90-beta and annexin A1 positively correlated and such co-overexpression of Hsp90-beta and annexin A1 contributed to lung cancer diagnosis. PMID: 25300907
  16. Hsp90 binds directly to fibronectin (FN) and inhibition reduces the extracellular fibronectin matrix in breast cancer cells. PMID: 24466266
  17. A novel mechanism for human carcinogenesis via methylation of HSP90AB1 by SMYD2. PMID: 24880080
  18. Hsp90 is upregulated in systemic sclerosis (SSc) and is critical for TGF-beta signalling. PMID: 23661493
  19. HSP70 was massively up-regulated in all mast cells three months after irradiation whereas HSP90AB1 was up-regulated only in a portion of mast cells PMID: 24670792
  20. Study obtained a structural model of Hsp90 in complex with its natural disease-associated substrate, the intrinsically disordered Tau protein. Hsp90 binds to a broad region in Tau that includes the aggregation-prone repeats. PMID: 24581495
  21. HSP90beta may positively regulate angiogenesis, not only as a protein chaperone, but also as an mRNA stabilizer for pro-angiogenic genes, such as BAZF, in a PRKD2 activity-dependent manner. PMID: 23515950
  22. Here we describe the specific association of heat shock protein-90-beta (Hsp90beta) with EV71 viral particles by the co-purification with virions using sucrose density gradient ultracentrifugation and colocalization as shown by immunogold EM. PMID: 23711381
  23. differences in expression caused by the -144 polymorphism in the HSP90beta promoter are associated with cellular inflammatory responses and the severity of organ injury PMID: 23516526
  24. the transdominant effect of HSP90AB1 on capsid-spacer protein 1-mutant HIV infectivity suggests a potential role for this class of cellular chaperones in HIV core stability and uncoating. PMID: 23200770
  25. The upregulation of Hsp90-beta was associated with poor post-surgical survival time and lymphatic metastasis of lung cancer patients PMID: 22929401
  26. DNA sequencing of 101 human samples detects eight and seven unique single nucleotide polymorphisms (SNPs) at the HSP90AA1 and HSP90AB1 loci, respectively. PMID: 22185817
  27. a possible role for HSP90AB1 in postentry HIV replication and may provide an attractive target for therapeutic intervention. PMID: 21602280
  28. TRIM8 modulates translocation of phosphorylated STAT3 into the nucleus through interaction with Hsp90beta and consequently regulates transcription of Nanog in embryonic stem cells. PMID: 21689689
  29. cyclophilin A and Hsp90 facilitate translocation of lethal factor(N) diphtheria toxin, but not of lethal factor, across endosomal membranes, and thus they function selectively in promoting translocation of certain proteins, but not of others PMID: 20946244
  30. Results show that RPL4, RPLP0, and HSPCB were the most stable reference genes in ovarian tissues. PMID: 20705598
  31. H. pylori induces the translocation of HSP90beta from the cytosol to the membrane and interaction of HSP90beta and Rac1, which leads to the activation of NADPH oxidase and production of ROS in gastric epithelial cells. PMID: 20451655
  32. findings present novel Hsp90 mutants that render cells resistant to Hsp90 inhibitors; show that the resistance depends on the increased ATPase turnover due to enhanced interaction with Aha1 PMID: 20226818
  33. Data show that the small molecule celastrol inhibits the Hsp90 chaperoning machinery by inactivating the co-chaperone p23, resulting in a more selective destabilization of steroid receptors. PMID: 19996313
  34. Study points to a potential role for Hsp90beta in MSC biology. PMID: 19327008
  35. PKC-epsilon is specifically required in the signaling pathway leading to the induction of hsp90 beta gene in response to heat shock. PMID: 14532285
  36. hsp90beta is repressed by p53 in UV irradiation-induced apoptosis PMID: 15284248
  37. Mutations at the phosphorylation sites of HSP90-beta modulate the interaction with arylhydrocarbon receptor (AhR) and may negatively regulate formation of the functional AhR complex in the steady-state cytosol. PMID: 15581363
  38. Hydrogen-exchange mass spectrometry was used to study structural & conformational changes undergone by full-length Hsp90beta in solution upon binding of the co-chaperone Cdc37 & 2 Hsp90 ATPase inhibitors: Radicicol & the anticancer drug DMAG PMID: 17764690
  39. Results suggest that HSP90 beta prevents auto-ubiquitination and degradation of its client protein c-IAP1, whose depletion would be sufficient to inhibit cell differentiation. PMID: 18239673
  40. These data provide an explanation for apoptosome inhibition by activated leukemogenic tyrosine kinases and suggest that alterations in Hsp90beta-apoptosome interactions may contribute to chemoresistance in leukemias. PMID: 18591256
  41. Presence of ovarian autoantibodies to human HSP90 in sera of women with infertility could be involved in human ovarian autoimmunity and thereby be a causative factor in early ovarian failure. PMID: 19022436
  42. Results show that heat shock protein 90 beta is cleaved by activated caspase-10 under UVB irradiation. PMID: 19380486
  43. celastrol may represent a new class of Hsp90 inhibitor by modifying Hsp90 C terminus to allosterically regulate its chaperone activity and disrupt Hsp90-Cdc37 complex. PMID: 19858214

Show More

Hide All

Subcellular Location Cytoplasm, Melanosome, Nucleus, Secreted, Cell membrane
Protein Families Heat shock protein 90 family
Database Links

HGNC: 5258

OMIM: 140572

KEGG: hsa:3326

STRING: 9606.ENSP00000325875

UniGene: Hs.509736


Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2021 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1