HSP90AB1 Antibody

Datasheet
Code CSB-PA00109A0Rb
See More Details Free Antibody trial simple
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western Blot
    Positive WB detected in: K562 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate
    All lanes: HSP90AB1 antibody at 2µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 84 kDa
    Observed band size: 84 kDa

  • IHC image of CSB-PA00109A0Rb diluted at 1:200 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of MCF-7 cells with CSB-PA00109A0Rb at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Product Details

Full Product Name Rabbit anti-Human HSP90AB1 Polyclonal antibody
Uniprot No. P08238
Target Names HSP90AB1
Alternative Names Heat shock protein HSP 90-beta (HSP 90) (Heat shock 84 kDa) (HSP 84) (HSP84), HSP90AB1, HSP90B HSPC2 HSPCB
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Heat shock protein HSP 90-beta protein (150-355AA)
Immunogen Species Human
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:200-1:500
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Citations

System-wide Profiling of RNA-Binding Proteins Uncovers Key Regulators of Virus Infection. Garcia-Moreno M, et al,Molecular Cell,2019

Applications: WB
Review: KO and overexpression of HSP90AB1 and SINV C accumulation (18 hpi) were assessed by western blotting.
PMID: 30799147

Cyclosporin A aggravates hydrogen peroxide-induced cell death in kidney proximal tubule epithelial cells. Daeun Moon, et al,Anatomy & Cell Biology,2019

Applications: WB
Antibody dilution factor: 1: 5000
Review: Expression levels of 94 kDa glucose-regulated protein (GRP94) and 78 kDa glucose-regulated protein (GRP78) were measured by western blot analysis. Antibody of β-actin (43 kDa) as a loading control was used for normalization.

Target Data

Function Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function
Subcellular Location Cytoplasm, Melanosome, Nucleus, Secreted, Cell membrane
Protein Families Heat shock protein 90 family
Database Links

HGNC: 5258

OMIM: 140572

KEGG: hsa:3326

STRING: 9606.ENSP00000325875

UniGene: Hs.509736

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