Human cyclophilin A (PPIA/CYPA) ELISA Kit_x000D_

Code CSB-E09920h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P62937
Lead Time 3-5 working days
Abbreviation PPIA
Protein Biological Process 1 Immunity
Target Name peptidylprolyl isomerase A (cyclophilin A)
Alias CYPA, CYPH, MGC117158, MGC12404, MGC23397, PPIase A|T cell cyclophilin|cyclosporin A-binding protein|peptidyl-prolyl cis-trans isomerase A|peptidylprolyl isomerase A|rotamase A
Species Homo sapiens (Human)
Protein Biological Process 3 Host-virus interaction
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported.
HGNC 9253
RGD 3372
MGI 3648545
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PPIA/CYPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 87-95  
1:2 Average % 100  
Range % 95-104  
1:4 Average % 90  
Range % 85-94  
1:8 Average % 98  
Range % 92-101  
Recovery
The recovery of human PPIA/CYPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 84-94  
EDTA plasma (n=4) 95 89-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
200 2.079 2.035 2.057 1.948  
100 1.594 1.576 1.585 1.476  
50 0.849 0.831 0.840 0.731  
25 0.446 0.439 0.443 0.334  
12.5 0.299 0.284 0.292 0.183  
6.25 0.204 0.198 0.201 0.092  
3.12 0.153 0.158 0.156 0.047  
0 0.110 0.108 0.109    
References
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Function PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Subcellular Location Cytoplasm, Secreted
Protein Families Cyclophilin-type PPIase family, PPIase A subfamily
Database Links

HGNC: 9253

OMIM: 123840

KEGG: hsa:5478

STRING: 9606.ENSP00000419425

UniGene: Hs.356331

Pathway Necroptosis

Q&A and Customer Reviews

Q&A
Q:

Can you provide me with the information if antibodies are polyclonal or monoclonal?

A:
Thanks for your inquiry. Pls check our information: CSB-E09920h: Coated anti: Mouse monoclonal antibody Detect anti: Goat polyclonal antibody; Pls let me know if you have any further questions. Thank you.
Customer Reviews

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