Human matrix metalloproteinase 3/stromelysin 1(MMP3/STR1) ELISA kit

Instructions
Code CSB-E04677h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name matrix metallopeptidase 3 (stromelysin 1, progelatinase)
Alternative Names CHDS6 ELISA Kit; Matrix metalloproteinase 3 ELISA Kit; Matrix metalloproteinase 3 preproprotein ELISA Kit; Matrix metalloproteinase-3 ELISA Kit; MGC126102 ELISA Kit; MGC126103 ELISA Kit; MGC126104 ELISA Kit; MMP 3 ELISA Kit; MMP-3 ELISA Kit; MMP3 ELISA Kit; MMP3_HUMAN ELISA Kit; Proteoglycanase ELISA Kit; SL-1 ELISA Kit; SL1 ELISA Kit; STMY ELISA Kit; STMY1 ELISA Kit; STR1 ELISA Kit; Stromelisin 1 ELISA Kit; Stromelysin 1 ELISA Kit; Stromelysin 1 progelatinase ELISA Kit; Stromelysin-1 ELISA Kit; Transin 1 ELISA Kit; Transin-1 ELISA Kit
Abbreviation MMP3
Uniprot No. P08254
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.054 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
Gene References into Functions
  1. BMAL1 Deficiency Contributes to Mandibular Dysplasia by Upregulating MMP3. PMID: 29276151
  2. rs3025058 in MMP3 and rs2276109 in MMP12 might not contribute to the risk of developing rheumatic heart disease in a Han population in Southern China. PMID: 29458338
  3. study to explore the relationship between 2 polymorphisms (MMP-1-755 T/G [rs498186] and MMP-3 A/C [rs632478]) and disc degeneration; a significant association was found between the MMP-3 polymorphism and disc degeneration; the homozygote CC was associated with an increased risk of disc degeneration compared with the AA genotype PMID: 28497435
  4. Higher serum MMP-3 levels in knee osteoarthritis reflect disease "generalization". PMID: 29164307
  5. Report a positive correlation between glomerular filtration rate and MMP-3 activity in diabetic patients. PMID: 29421776
  6. The maternal and fetal MMP3 gene polymorphisms may be strong genetic markers of preeclampsia, occurring either individually or together. PMID: 29670668
  7. Preliminary studies indicate that baseline MMP3 and TIMP3 concentrations are associated with patient survival and disease-free time PMID: 29304854
  8. MMP-3 (Lys45Glu) polymorphisms associate with obesity risk and its severity. PMID: 29317790
  9. Data suggest that serum matrix metalloproteinase-3 (MMP-3) and the 7-joint ultrasound score (US7) scores could both effectively reflect disease activity and therapeutic responses in patients with moderate to severe rheumatoid arthritis (RA). PMID: 29141665
  10. results demonstrated that measurement of MMP-3 could become a marker of disease activity in rheumatoid arthritis patients PMID: 28461705
  11. The results indicated that the 5A allele of the MMP3 gene-promoter region could be associated with oral submucous fibrosis risk factor, but not oral squamous cell carcinoma, in an Indian population PMID: 27389714
  12. C/EBPbeta upregulation promoted tumor cell invasion in an MMP3-dependent manner in vitro and was associated with metastatic status in colorectal cancer PMID: 29172775
  13. MMP3 (and MMP-1 and MMP-2) are independently associated with markers of arterial stiffening in patients with type 1 diabetes. PMID: 29070037
  14. A significant increase in the frequency of the MMP3-1171dupA (rs3025058) polymorphism genotype among patients with unstable angina was detected. PMID: 29044936
  15. No interaction between MMP-3 5A/6A polymorphism and the risk of recurrence in total IS patients was found. The variant 5A/6A+5A/5A genotype and the 5A allele were significantly associated with a high risk of recurrence for large-artery atherosclerosis (LAA) (multivariate-adjusted, P=0.002, 0.001, respectively), but not for small-artery occlusion and cardioembolism. PMID: 28843428
  16. The 5A allele of the MMP3-gene promoter polymorphism is a potential risk factor in the poor outcome of hemodialysis patients. PMID: 28781337
  17. Positive MMP-3 was not significantly related to osteoporosis or severe hand osteoarthritis. PMID: 28365812
  18. In patients with ankylosing spondylitis, serum MMP3 level was found to have a positive correlation with the MRI score of sacroiliac joint and c-reactive protein. PMID: 28432524
  19. Suppression of active MMP3, but not total circulating MMP3 was associated with treatment response in rheumatoid arthritis patients treated with tocilizumab. PMID: 28850021
  20. These results, for the first time, demonstrate that P15 binding to cell surface vimentin inhibits the tumor cell invasion and is associated with reduced MMP3 expression. Thus, suggesting that P15 has potential as an anti-metastatic therapy in pancreatic cancer. PMID: 28396463
  21. A Study of IL-1beta, MMP-3, TGF-beta1, and GDF5 Polymorphisms and Their Association with Primary Frozen Shoulder in a Chinese Han Population PMID: 28676856
  22. studies establish a molecular imaging reporter for diagnosing early-stage EOC. Additional studies are required to confirm the early-stage activity of MMP-3 in EOC and its diagnostic and prognostic significance PMID: 29390034
  23. among Chinese males, MMP-3 rs650108 and MMP-8 rs2012390 decrease alcohol-induced ONFH risk and MMP-8 rs11225394 increases it. PMID: 28445942
  24. Report an association between the MMP-3 rs3025058 and subclinical markers of carotid and coronary atherosclerosis at the time of recruitment. MMP-3 rs3025058 on affects carotid artery disease progression in the 3.8-year follow-up in patients with T2DM. PMID: 28521653
  25. promoter polymorphism rs3025058 associated with adolescent idiopathic scoliosis but not with gender bias PMID: 27911281
  26. mRNA levels of HSP family members (HSP70B', HSP72, HSP40/DNAJ, and HSP20/CRYAB) are upregulated by the intracellular MMP3 overload. PMID: 27206651
  27. These observations suggest a crucial role for cancer-associated fibroblasts and fibroblast growth factor-1/fibroblast growth factor receptor 4 signaling in the progression of ovarian cancer. the expression level of Snail1 and MMP3 was reduced, while the expression level of E-cadherin increased PMID: 28718374
  28. MMP3 overexpression is associated with Melanoma Tumor Growth and Metastasis. PMID: 27013197
  29. Matrix metalloprotease (MMP) regulation was the top pathway involved in gingival aging. MMP3, MMP9, MMP12, and MMP13 were upregulated in old gingival tissues, concomitantly with interleukin-1 beta (IL1B) expression. PMID: 27391467
  30. Three previously investigated MMP3 variants (rs679620, rs591058 and rs650108) in addition to the functional promoter variant (rs3025058) were genotyped in 195 Australian control participants and 79 Australian individuals with chronic Achilles tendinopathy. PMID: 27211292
  31. MMP-3 expression is deregulated in osteosarcomas and this potentially contributes to metastasis and might be a promising marker for the prognosis and therapy of metastatic osteosarcoma. PMID: 27837634
  32. These results indicate that periodic induction, via use of an eye drop, of AAV-mediated secretion of MMP-3 into aqueous humour could have therapeutic potential for those cases of glaucoma that are sub-optimally responsive to conventional pressure-reducing medications. PMID: 28158775
  33. In summary, our study demonstrates that IL-7/IL-7R axis promotes the invasion and migration of prostate cancer cells, through activation of AKT/NF-kappaB pathway and upregulation of MMP-3 and MMP-7 expression PMID: 27611862
  34. Study showed that serum MMP-3 levels are decreased in patients with epilepsy, which is relevant for acute stroke. A specific pathogenic effect of MMP-3 in epilepsy appears unlikely. This study has demonstrated that the newly developed Luminex assays are useful in quantifying MMP-3 levels in human serum. PMID: 27423606
  35. Serum baseline levels of MMP-3 are strong prognostic markers of disease activity, and act well as an early predictor of progressive joint damage in recent-onset rheumatoid arthritis. PMID: 25292349
  36. MMP-3 positivity has a strong relationship with meningiomas having an aggressive character; MMP-3 may be used as a proliferation marker for biological behaviour, recurrence rate and prognosis of meningiomas PMID: 27438616
  37. 5A5A genotype of MMP-3-1171 5A/6A gene polymorphism was associated with adolescent idiopathic scoliosis, especially in Caucasian population. (Meta-analysis) PMID: 27659327
  38. PAF stimulation dose-dependently promoted the invasion, migration and growth of prostate cancer cells in vitro, while knockdow our findings demonstrate that PAFR can activate ERK1/2 pathway, and subsequently increase MMP-3 expression and decrease E-cadherin expression PMID: 27176648
  39. 5A/6A polymorphism of matrix metalloproteinase-3 may contribute to recurrent atherosclerotic ischemic stroke in Chinese. PMID: 26314579
  40. We concluded that overexpression of MMP-3 and uPA, altogether with diminished expression of PAI-1 from metastatic tumors, might be a crucial step towards metastasis in ductal breast cancer. PMID: 27975070
  41. NFAT1 silencing could suppress cell migration and invasion through MMP-3. PMID: 28024290
  42. Specific gene polymorphisms are known to be associated with a different arterial physiology in the younger generation. The present study found that young Russians with the matrix metalloproteinase 3 6A/6A and gamma-glutamyltransferase 1AA genotypes have lower levels of the cardio-ankle vascular index - a recent measure of arterial stiffness. PMID: 27161754
  43. MMP-3 -11715A/6A polymorphism was associated with MMP-3 expression in herniated disc tissues. MMP-3 expression and the histological and radiological scores were positively correlated. PMID: 27706715
  44. High MMP3 expression is associated with Ovarian Tumors. PMID: 27268637
  45. MMP3 gene -1171 5A/6A polymorphism and upregulated protein expression may be associated with deep vein thrombosis risk in Chinese Han population. PMID: 27177702
  46. data demonstrated that the MMP3 rs650108 variant was significantly associated with increased frozen shoulder susceptibility in a Chinese Han population. PMID: 27051023
  47. MMP3 is released from the synovial membrane under inflammatory conditions and may serve as biological markers for inflammatory osteoarthritis. PMID: 26863055
  48. This study showed an increased frequency of heterozygotes for stromelysin-1 rs3025058 and thought to be involved in the etiology of Gastric cancer PMID: 26853425
  49. These results indicate an essential role for MAPKs in the induction of MMP-3 in synovial sarcoma cells, through AP-1 activation. PMID: 26850593
  50. A low level of MMP-3 is an excellent positive predictor for polymyalgia rheumatica with giant cell arteritis. PMID: 26156043
  51. These results demonstrated widespread alterations of matrix metalloproteinase 3 expression in Multiple System Atrophy. PMID: 26260627
  52. MMP-3 haplotypes association with susceptibility and severity of juvenile idiopathic arthritis PMID: 26510114
  53. increased serum MMP-3 level correlates with the development of AS, suggesting that MMP-3 may present a clinical value in reflecting the progression of AS. PMID: 26681054
  54. MMP-3 is associated with the occurrence and development of RA bone erosion, and its serum level and mRNA expression can be treated as important predictors of joint damage. PMID: 26634533
  55. new evidence that promoter polymorphisms in MMP3 are functional and may affect gene transcription with possible effects on craniofacial development leading to NSCL/P. PMID: 24799419
  56. Elevated levels of MMP-3 are associated with an increased risk of long-term MI in patients with and without ACS referred for coronary angiography. PMID: 26805660
  57. MMP3 rs522616 in the promoter region was significantly associated with coal workers' pneumoconiosis PMID: 26528997
  58. The MMP-3-1612 5A/6A polymorphism may effect serum MMP-3 level and over-expression of serum MMP-3 level may be a risk factor for DVT formation. PMID: 27129069
  59. MMP-3 Is an Endogenous Activator of the MMP-9 Secreted by Placental Leukocytes: Implication in Human Labor PMID: 26713439
  60. MMP3 -1171 5A/6A polymorphism contributes to a decreased susceptibility to chronic periodontitis, especially in Asian populations. PMID: 26123623
  61. Meta-analysis of matrix metalloproteinase-3 gene polymorphism revealed 6A allele and 6A/6A genotype significantly increased the risk of moyamoya disease. PMID: 25564266
  62. MMP-3 level was shown to be useful as a disease activity marker in rheumatoid arthritis patients PMID: 27089651
  63. the G allele and AG genotype of MMP-3 E45K polymorphism is associated with increased risk of osteosarcoma in adolescent population of Turkey. PMID: 27145630
  64. Our results demonstrated that MMP-3 expression is deregulated in osteosarcomas PMID: 27061553
  65. Gene encoding MMP3 was identified as being upregulated and found to be negatively correlated with survival over 3-year follow-up period in Idiopathic pulmonary fibrosis. PMID: 26109466
  66. MMP-3 polymorphism has a significant association with the risk of developing morbidity after acute myocardial infarction. PMID: 26712881
  67. The rs1800796 of IL-6 and rs679620 of MMP-3 were found significantly associated with increased susceptibility and severity of post-operative stiffness after rotator cuff repair PMID: 26191285
  68. MMP3 polymorphisms were not associated with primary ovarian insufficiency in Korean women. PMID: 25482733
  69. TNF-alpha, VEGF, and MMP-3 might directly participate in the differentiation of fibroblasts into osteoblasts in ankylosing spondylitis. PMID: 26125893
  70. Data show that matrix metallopeptidase 3 (MMP-3) plasma levels were higher in men than in women, and MMP-3 single nucleotide polymorphisms (SNPs) allele 6A carriers also had higher levels than allele 5A carriers, an effect that was magnified by sepsis. PMID: 26206176
  71. Serum MMP3 was significantly higher in juvenile-onset anklylosing spondylitis patients than in controls. There was no significant difference in MMP3 levels among patients with various HLA-B27 genotypes. MMP3 level was associated with disease activity. PMID: 25912212
  72. MT3 may regulate breast cancer cell invasiveness by modulating the expression of MMP3. PMID: 25933064
  73. Up-regulation of matrix metalloproteinase 3 is associated with colitis-associated cancer. PMID: 25742789
  74. Genetic variants of NOS3, CD14, MMP3 and IL4R are implicated in the determination of VEGF expression and plasma levels. PMID: 26437765
  75. For MMP3, G was undertransmited among affected progeny (p=0.019) in cleft palate subgroup. PMID: 25463899
  76. The present study investigated changes of circulating metalloproteinases (MMP-3, MMP-9) and CRP levels in patients with ulcerative colitis and Crohn's disease. PMID: 25611261
  77. Cysteinerich angiogenic inducer 61 regulates MMP-3 expression. PMID: 25351421
  78. a lack of correlation between MMP-3-1171 5A/6A polymorphism and rheumatoid arthritis susceptibility (Meta-Analysis) PMID: 25403368
  79. MMP3, known to be expressed in PDA, was found to be associated with expression of Rac1b, a tumorigenic splice isoform of Rac1, in all stages of pancreatic cancer. PMID: 24850902
  80. No association was found between MMP3 G/A rs679620 variants and anterior cruciate ligament rupture. PMID: 21410539
  81. The determination of MMP-3 -1171 5A/6A polymorphism may be recommended for the early prediction of a risk for the development and severe course of CHF. PMID: 26087627
  82. miR-93 contributed to abnormal nucleus pulposus cell type II collagen expression by targeting MMP3, involved in intervertebral disc degeneration. PMID: 25818544
  83. Significant SNPs in ACE and MMP3 also showed association with Alzheimer's disease (AD) risk PMID: 25340798
  84. Patellar tendon properties are not influenced by the MMP3 gene variants measured. Although MMP3 gene variants are associated with risk of tendon pathology, association is unlikely to be mediated via underlying tendon dimensional and functional properties. PMID: 25168209
  85. Homozygocity for MMP3 rs3025058 and rs678815 polymorphisms is a potential marker of enhanced susceptibility to lung cancer development among COPD subjects. PMID: 25292126
  86. Bidirectional approach based on both serum MMP-3 level and US scores can further improve the assessment of disease activity in rheumatoid arthritis patients. PMID: 24670135
  87. Plasma MMP-3 levels remained significantly elevated from baseline for 4 weeks after minimally invasive colorectal resection for colorectal cancer. PMID: 24939159
  88. IGF-II, TGF-beta1 and VEGF-A and its receptor in malignant tumor tissue, as well as increasedplasmin release from proenzyme and MMP-3 activationis apparently associated with the formation of pathogenic mechanism of vasculature development PMID: 25993872
  89. Serum MMP-3 may be an alternative noninvasive biomarker of histological synovitis and RA diagnosis. PMID: 25147433
  90. The albumin-thiol redox state was significantly oxidized in correlation with serum MMP-3 elevation in rheumatoid arthritis PMID: 25807652
  91. MMP3 may be the recommended urinary metalloproteinases as early diagnostic biomarker in the early stages of both types of bladder cancer although both MMP9 and MMP3 can be used in the diagnosis of advanced stages. PMID: 25135219
  92. High MMP3 stromal protein expression is highly specific for the tumorous transition in cases of sporadic colorectal neoplasias. PMID: 24999605
  93. We found significant differences in the incidence of particular MMP3 and MMP9 alleles, which substantiate the role of matrix metalloproteinases in EGE pathogenesis. PMID: 25711032
  94. beta6 induced phosphorylation of ETS1 via the ERK/MAPK pathways, through which the MMP-3/MMP-9 promoters were stimulated, thereby leading to the up-regulation of MMP-3/MMP-9, and subsequent the invasion of colon cancer cells. PMID: 25135220
  95. MMP3 and CXCL13 are systemic biomarkers that reflect the aggregate burden of these pathological features across total lung tissue. PMID: 25217476
  96. This study provided evidence that the mutant 5A allele of MMP-3 is a crucial risk factor for the severity of lumbar disc degeneration. PMID: 23975061
  97. Analysis of gene polymorphisms of proteolytic enzymes and inhibitors of the enzyme gene showed statistically significant differences between patients with COPD and lung cancer only for the MMP3 gene 6A/5A. PMID: 23744166
  98. ROCKi decreased the association between ETS-1 and its binding sites on the MMP-3 promoter, whereas ROCKi promoted the interaction between SOX9 and the AGN promoter. PMID: 24111521
  99. Polymorphism of MMP-3 - rs3025058 in patients with Hypertrophic Cardiomyopathy was determined.Allele variant MMP-3 1171 was associated with hypertrophy of interventricular septum. PMID: 25177880
  100. The -1171(5A>6A) polymorphism in MMP3 promoter region is not associated with overall cancer risk. PMID: 24489939

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Involvement in disease Coronary heart disease 6 (CHDS6)
Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families Peptidase M10A family
Database Links

HGNC: 7173

OMIM: 185250

KEGG: hsa:4314

STRING: 9606.ENSP00000299855

UniGene: Hs.375129

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