Human progesterone receptor,PGR ELISA Kit

Instructions
Code CSB-E09214h
Size 96T,5×96T,10×96T

Product Details

Target Name progesterone receptor
Alternative Names NR3C3, PR,
Abbreviation PGR
Uniprot No. P06401
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.29 ng/mL-10 ng/mL
Sensitivity 0.14 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays):CV%<15%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PGR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 96    
Range % 90-110    
1:2 Average % 93    
Range % 91-100    
1:4 Average % 89    
Range % 86-98    
1:8 Average % 95    
Range % 86-98    
Recovery
The recovery of human PGR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 96 91-98    
EDTA plasma (n=4) 97 93-100    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average Corrected    
10 1.565 1.579 1.572 1.530    
3.6 0.663 0.652 0.658 0.616    
1.4 0.364 0.355 0.360 0.318    
0.7 0.198 0.202 0.200 0.158    
0.3 0.102 0.105 0.104 0.062    
0 0.041 0.042 0.042      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Depending on the isoform, progesterone receptor functions as transcriptional activator or repressor.
Subcellular Location Nucleus, Cytoplasm, Note=Nucleoplasmic shuttling is both homone- and cell cycle-dependent, On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases, SUBCELLULAR LOCATION: Isoform A: Nucleus, Cytoplasm
Protein Families Nuclear hormone receptor family, NR3 subfamily
Tissue Specificity In reproductive tissues the expression of isoform A and isoform B varies as a consequence of developmental and hormonal status. Isoform A and isoform B are expressed in comparable levels in uterine glandular epithelium during the proliferative phase of th
Database Links

HGNC: 8910

OMIM: 607311

KEGG: hsa:5241

STRING: 9606.ENSP00000325120

UniGene: Hs.32405

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