Human soluble CD163(sCD163) ELISA Kit

Code CSB-E14050h
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human CD163 ELISA Kit was designed for the quantitative measurement of Human CD163 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

Target Name CD163 molecule
Alternative Names C163A_HUMAN ELISA Kit; CD 163 ELISA Kit; CD163 ELISA Kit; CD163 antigen ELISA Kit; CD163 molecule ELISA Kit; Hemoglobin scavenger receptor ELISA Kit; M130 ELISA Kit; M130 antigen precursor ELISA Kit; Macrophage associated antigen ELISA Kit; MM130 ELISA Kit; OTTHUMP00000238617 ELISA Kit; OTTHUMP00000238618 ELISA Kit; OTTHUMP00000238619 ELISA Kit; OTTHUMP00000238620 ELISA Kit; SCARI1 ELISA Kit; Scavenger receptor cysteine rich type 1 protein M130 ELISA Kit; sCD163 ELISA Kit; Soluble CD163 ELISA Kit
Abbreviation CD163
Uniprot No. Q86VB7
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 ng/mL-100 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human sCD163 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:5Average %88
Range %83-93
1:10Average %95
Range %89-100
1:20Average %97
Range %92-103
1:40Average %95
Range %91-99
Recovery
The recovery of human sCD163 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-99
EDTA plasma (n=4)9893-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
1002.452 2.552 2.502 2.368
502.134 2.176 2.155 2.021
251.539 1.497 1.518 1.384
12.50.728 0.739 0.734 0.600
6.250.412 0.424 0.418 0.284
3.120.229 0.239 0.234 0.100
1.560.194 0.201 0.198 0.064
00.132 0.135 0.134  
Materials provided
  • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human CD163 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled CD163 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
  • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
  • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.; After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
Gene References into Functions
  1. high plasma levels of sCD163 is associated with measles virus infection. PMID: 29795672
  2. Increased sCD163 levels in sputum are associated with the impairment of lung function in asthma patients PMID: 30246699
  3. Expression of CD163 in urinary bladder cancer cells is significantly correlated with macrophage infiltration and might have a tumor promoting impact. PMID: 29288002
  4. A separate measurement of the two forms of CD163 constituting 'soluble CD163' in plasma may therefore add to the diagnostic and prognostic value. PMID: 28084321
  5. our results suggest that MR and CD163 may be novel biomarkers for CRC detection and survival evaluation. PMID: 29226859
  6. Data suggest a novel pathophysiological role of CD163 in type 2 diabetes; monocyte surface CD163 levels are significantly associated with insulin resistance in patients with type 2 diabetes; the association of insulin resistance with soluble CD163 levels is less significant. This study was conducted in Japan. PMID: 29445750
  7. Detection of the CD163 antigen on cancer cells might be a useful marker for evaluating the clinical course of patients with clear cell renal cell carcinoma PMID: 28687956
  8. CD163-expression by cancer cells was significantly associated with MI and clinicopathological data. Patients with CD163-positive tumors had significantly shorter disease-free survival (DFS) after RT. PMID: 29725763
  9. Plasma CD163 levels were increased in obese and type 2 diabetes mellitus patients compared to controls. PMID: 28406702
  10. soluble CD163 is associated with markers of liver necro-inflammation and glucose homoeostasis in non-alcoholic fatty liver disease. PMID: 28715286
  11. The effect of LPS in the expression of CD163 and CD206 on monocytes is not reverted in LPS tolerant cells, and the inhibition of inflammatory cytokines in tolerant cells is not related with modulation of these receptors PMID: 26352275
  12. The cumulative incidence of de novo-onset cGVHD was higher in patients with higher plasma soluble CD163 concentrations at day 80 than those with lower concentrations (75% versus 40%, P = .018). The cumulative incidence of de novo- or quiescent-onset cGVHD did not differ statistically according to concentrations of the 3 other proteins at day 80. PMID: 28455006
  13. Although sCD163 level cannot predict the development of varices, it serves as a good predictor for the detection of size of varices (large varices), the need of prophylactic interventions, and risk of variceal bleeding. sCD163 level is a helpful indicator with the progression of cirrhosis and portal hypertension. PMID: 28282272
  14. CD163 and ferritin are biomarkers of functional outcomes in intracerebral hemorrhage. PMID: 28392117
  15. the simultaneous detection of both plasma sCD14 and IL-6 is a promising diagnostic approach to identify active pulmonary tuberculosis, and further, measurement of TNF-alpha and sCD163 can identify the most severe cases of tuberculosis. PMID: 27591510
  16. The macrophage activation marker CD163 was increased in patients with active pulmonary TB compared with age- and sex-matched control subjects. PMID: 27684274
  17. alveolar macrophages from asthmatic subjects have reduced cell-surface expression of CD163, which suggests that CD163 might modulate the pathogenesis of asthma PMID: 26376364
  18. Serum sCD163 might be a potential biomarker for predicting the severity and prognosis of PM/DM-related interstitial lung disease PMID: 28103926
  19. Soluble CD163 reflecting macrophage activation is associated with morphological features of non-alcoholic fatty liver disease. An independent association between sCD163 and cytokeratin-18 suggests that apoptosis may contribute to macrophage activation in NAFLD/NASH. PMID: 27102725
  20. Most cases of histiocytic sarcoma expressed histiocytic markers CD68 (6 of 7 cases), CD163 (5 of 5 cases), and PU.1 (3 of 4 cases). PMID: 28805986
  21. Within a diabetic cohort, the percentage of monocytes expressing CD163 was decreased in those subjects with complications, compared to those without complications. These changes were not accompanied by a change in gene expression. Glucocorticoids increased CD163 expression in cultured peripheral blood monocytes from non-diabetic persons. PMID: 27354410
  22. sCD163 was markedly elevated in autoimmune hepatitis (AIH) in the acute phase, normalised by successful treatment in complete responders, but remained higher in the incompletely responding cases. Our results demonstrate macrophage activation in AIH paralleling disease activity, severity and treatment response, suggesting a role for macrophage activation in AIH. PMID: 27679428
  23. High CD163 expression is associated with glomerular inflammation in lupus nephritis. PMID: 27242373
  24. analysis of plasma CXCL10, sCD163 and sCD14 in virological suppression and risk of cardiovascular disease PMID: 27355513
  25. we found that higher percentages of circulating CD14+CD204+, CD14+CD163+CD204+ M2-like monocytes were significantly associated with TNM stage, lymph node metastasis, and histological differentiation. PMID: 28639912
  26. CD163 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
  27. Soluble CD163, which is identified as a marker of inflammation and type II diabetes, is elevated in polycystic ovary syndrome. PMID: 26985764
  28. soluble CD163 modulates the immune response to Dermatophagoides pteronyssinus allergens potentiating anti-inflammatory, homeostatic mechanisms. PMID: 27100336
  29. The mean serum-soluble CD163 level was higher in the ANCA-associated renal vasculitis patients with infectious complications than in the active-vasculitis patients, inactive-vasculitis patients, and normal controls. PMID: 27094919
  30. we have developed a highly sensitive targeted probe capable of detecting CD163-expressing macrophages that could provide useful information about the state of the atheromatous lesions. PMID: 26616677
  31. A subset of CD163+ macrophages displays mixed polarizations in discoid lupus skin. PMID: 26568320
  32. Increased soluble CD163 blood levels in hepatocellular carcinoma patients correlates with rapid hepatocellular carcinoma progression. PMID: 26549495
  33. these data indicate that sCD163 is a prognostic marker in B-CLL. PMID: 25747973
  34. sCD163 is a sensitive marker protein for liver failure. PMID: 26339412
  35. CD163-positive macrophages predict outcome in follicular lymphoma, but their prognostic impact is highly dependent on treatment received. PMID: 25869385
  36. therapy with rituximab, cyclophosphamide and fludarabine resulted in a significant reduction in the number of non-classical CD14+CD16++ monocytes and soluble form of CD163 but upregulation of membrane-associated monocyte CD163 PMID: 26135617
  37. Renal graft CD163+ infiltrates correlated strongly with interstitial inflammation, tubulitis, and peritubular capillaritis. PMID: 26087825
  38. Higher levels of circulating monocyte-platelet aggregates are correlated with viremia and increased sCD163 levels in HIV-1 infection. PMID: 25109683
  39. CD-163, related to immune-system (macrophage), correlated with symptoms (pain or discomfort) of prostatic inflammation. PMID: 26045748
  40. The correlation of CD antigens with cytomegalovirus (CMV) antibodies in HIV positive people showed that increased CD163 but not CD14 is a marker of CMV infection. PMID: 26569178
  41. Soluble CD163 is increased in patients with acute pancreatitis. PMID: 26209500
  42. CD163 may be correlated with disease severity and the disease progression in patients with hemorrhagic fever with renal syndrome; however, the underlying mechanisms should be explored further. PMID: 25392926
  43. Suggest MelanA-negative spindle-cell associated melanoma constitutes a distinct inflammatory phenotype correlated with dense infiltration of CD163 macrophages and loss of E-cadherin. PMID: 25602697
  44. The scavenger receptor CD163 has been found to be over-expressed in Head and neck squamous cell carcinoma. PMID: 24858806
  45. Show a high percentage of CD163+ tumor-associated macrophages and a low percentage of S100+ dendritic cells in thymic carcinoma samples. PMID: 25499804
  46. CD163 is a biomarker for the clinical assessment of macrophage proliferation and activity that would help in risk prediction of bone and lung involvement and monitoring treatment response PMID: 25587690
  47. This study describe elevated soluble CD163 (sCD163)levels in gestational diabetes mellitus (GDM) and identify human placenta as a novel source of sCD163 suggesting that placental tissues might contribute to the increased levels of circulating sCD163 in GDM pregnancies. PMID: 24983948
  48. could be potentially used as an immune diagnostic marker for malignant pleural effusion PMID: 25944005
  49. Soluble CD163 may be a marker of "unhealthy" fat distribution in obese subjects. PMID: 25376508
  50. IL-8 might be involved in poor clinical outcomes via generation of CD163-positive M2 macrophages, and these factors in addition to N status might have prognostic value in patients with resectable oral squamous cell carcinoma PMID: 25461761

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Subcellular Location [Soluble CD163]: Secreted.; Cell membrane; Single-pass type I membrane protein. Note=Isoform 1 and isoform 2 show a lower surface expression when expressed in cells.
Tissue Specificity Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed a
Database Links

HGNC: 1631

OMIM: 605545

KEGG: hsa:9332

STRING: 9606.ENSP00000352071

UniGene: Hs.504641

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