Mouse Interleukin 16,IL-16 ELISA KIT

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background

Product Details

Target Name

interleukin 16 (lymphocyte chemoattractant factor)

Alternative Names

Il16 ELISA Kit; Pro-interleukin-16 [Cleaved into: Interleukin-16 ELISA Kit; IL-16 ELISA Kit; Lymphocyte chemoattractant factor ELISA Kit; LCF)] ELISA Kit

Abbreviation

IL16

Uniprot No.

O54824

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

7.8 pg/mL-500 pg/mL

Sensitivity

1.95 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	80-99
1:2	Average %	96
	Range %	91-101
1:4	Average %	95
	Range %	92-100
1:8	Average %	98
	Range %	86-115

Recovery

The recovery of mouse IL-16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	80-115
EDTA plasma (n=4)	95	90-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.750	2.800	2.775	2.678
250	2.058	2.148	2.103	2.006
125	1.258	1.366	1.312	1.215
62.5	0.785	0.840	0.813	0.716
31.2	0.520	0.482	0.501	0.404
15.6	0.385	0.383	0.384	0.287
7.8	0.196	0.199	0.198	0.101
0	0.098	0.096	0.097

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Mouse IL16 ELISA Kit was designed for the quantitative measurement of Mouse IL16 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL .

Target Background

Function
(From Uniprot)

Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.; Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.; Isoform 2 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.

Gene References into Functions

The production of IL-16 contributed to lung damage as neutralization of IL-16 enhanced S. aureus clearance and resulted in diminished lung pathology in S. aureus pneumonia. PMID: 24736233
Data indicate that knockdown of translocator protein TSPO by siRNAhad showed no effect on the productions of TNF-alpha, IL-1beta and IL-6 in lipopolysaccharide (LPS)-stimulated BV-2 microglia cells. PMID: 25200148
Interleukin-16 promotes cardiac fibrosis and myocardial stiffening in heart failure with preserved ejection fraction. PMID: 23894370
Data show overexpression of pro-IL-16 impaired resting B cell proliferation mediated through regulating nuclear factor (NF)-kappaB activation, and siRNA knock-down of pro-IL-16 expression decreased the level of p27(kip) and increased the level of Skp2. PMID: 23297678
We determined that ovalbumin (OVA)-sensitized mice have an increase in systemic tumor necrosis factor-alpha levels, and that serum or BAL fluid stimulation of bronchial epithelial cells results in production of IL-16 PMID: 12594062
IL-16 induces a greater migratory response in the Th1 cell subset than in the Th2 subset. PMID: 14607889
interleukin-16 and its precursor have roles in T lymphocyte activation and growth [review] PMID: 15253385
IL-16 precursor mRNA and protein expression are dynamically regulated during CD4+ T cell activation by a calcineurin-dependent mechanism; pro-IL-16 may influence T cell cycle regulation, although not in a dominant manner. PMID: 15728482
Caspase-3 mediated production of IL-16 by infiltrating CD4+ T cells, contributes to ongoing neuroinflammation by chemoattraction of additional waves of CD4+ T cells. PMID: 16271292
After femoral artery ligation, CD8+ T cells infiltrate the site of collateral vessel growth and recruit CD4+ mononuclear cells through the expression of IL-16 PMID: 16380545
Inactivation of IL-16 by antibody therapy or IL-16 deficiency prevented ischemia-reperfusion injury of the kidney, as shown by reduced levels of serum creatinine or blood urea nitrogen. PMID: 18004294
Generation of bleomycin-induced lung fibrosis is independent of IL-16 PMID: 19232499
CD4-positive T cells are specifically recruited to the lung in a dendritic cell-independent but IL-16-dependent process and diminish neutrophil recruitment through increased IL-10 production. PMID: 19641139

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Subcellular Location

Secreted.; [Isoform 1]: Cytoplasm. Note=Colocalizes with GRIN2C in neuronal cell bodies and neurites.; [Isoform 2]: Cytoplasm. Nucleus.

Tissue Specificity

Isoform 1 is expressed in neurons of the cerebellum and hippocampus. Isoform 2 is expressed in thymus, spleen and lung.

Database Links

KEGG: mmu:16170

STRING: 10090.ENSMUSP00000001792

UniGene: Mm.10137

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Code	CSB-E04606m
Size	96T,5×96T,10×96T
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.