Mouse Perforin/Pore-forming protein,PF/PFP ELISA Kit

Code CSB-E13429m
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
perforin 1 (pore forming protein)
Alternative Names
Prf1 ELISA Kit; Pfp ELISA Kit; Perforin-1 ELISA Kit; P1 ELISA Kit; Cytolysin ELISA Kit; Lymphocyte pore-forming protein ELISA Kit
Uniprot No.
Mus musculus (Mouse)
Sample Types
serum, plasma, cell culture supernates
Detection Range
7.8 pg/mL-500 pg/mL
1.95 pg/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PF/PFP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:20 Average % 98  
Range % 93-105  
1:40 Average % 93  
Range % 85-100  
1:80 Average % 86  
Range % 81-93  
1:160 Average % 93  
Range % 87-99  
The recovery of mouse PF/PFP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 90-99  
EDTA plasma (n=4) 87 84-93  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
500 2.172 2.084 2.128 1.972  
250 1.786 1.693 1.740 1.584  
125 1.302 1.267 1.285 1.129  
62.5 0.785 0.823 0.804 0.648  
31.25 0.564 0.572 0.568 0.412  
15.6 0.362 0.349 0.356 0.200  
7.8 0.260 0.262 0.261 0.105  
0 0.157 0.154 0.156    
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Mouse PRF1 ELISA Kit was designed for the quantitative measurement of Mouse PRF1 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

(From Uniprot)
Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
Gene References into Functions
  1. Study in mice models shows that perforin from antigen-specific cytotoxic T lymphocytes is required for Nlrp3 inflammasome activation in antigen-presenting cells. Furthermore, such activation of NLRP3 inflammasome contributes to the induction of antigen-specific antitumour immunity and pathogenesis of graft-versus-host diseases. PMID: 28537251
  2. levetiracetam can still protect neurons with perforin knockout mice. PMID: 26454821
  3. These studies indicate that CD8+ T cells against a single antigen can restrict Y. pseudotuberculosis colonization in a perforin-dependent manner, but ultimately are insufficient in their ability to provide sterilizing immunity and protect against death. PMID: 27268148
  4. Furthermore, perforin production specifically by CD8 T cells was required to cause fatal edema during experimental cerebral malaria. PMID: 28264905
  5. Our study suggests that perforin plays a role in dopaminergic neuron loss in PD. PMID: 28137589
  6. study shows that perforin is essential to facilitate beta cell destruction in mouse models of type 1 diabetes PMID: 26446877
  7. Released granzyme B induces DNA fragmentation in intraepithelial lymphocytes independently of Perforin PMID: 25750028
  8. serglycin plays a critical role in the maturation of dense-core cytotoxic granules in cytotoxic lymphocytes and the trafficking and storage of perforin and granzyme B, whereas granzyme A is unaffected PMID: 26756195
  9. This suggests that LPS alters UNK cell migration and activates cytotoxic granule release. PMID: 26066976
  10. it is proposed that Ca(2+) binding at the weakest affinity site triggers changes in the perforin C2 domain that facilitate its interaction with lipid membranes PMID: 26306037
  11. a lack of perforin and absence of the specific activation of NK cells during acute MCMV infection lead to an unleashed CD8(+) T cell response that is detrimental for the host. PMID: 25809566
  12. CD8 T cells are sufficient as a sole perforin-expressing cell type to cause BBB disruption in the PIFS model. PMID: 25337791
  13. The rate and proportion of donor lymphoid cell engraftment and expansion of effector memory donor T cells were significantly increased within 5 to 7 days post-bone marrow transplantation in perforin-deficient recipients, compared with wild-type. PMID: 25459639
  14. Regulatory effects of perforin on glucose tolerance are mechanistically linked to the control of T-cell proliferation and cytokine production in inflamed visceral adipose tissue. PMID: 25048196
  15. Perforin (and granzyme B)-dependent apoptosis increases postapoptotic necrosis and inflammation in atherosclerosis. PMID: 25398236
  16. Perforin has a role in atherosclerotic plaque development. PMID: 24205352
  17. Defining the interaction of perforin with calcium and the phospholipid membrane. PMID: 24070258
  18. miR-150 is a common post-transcriptional regulator for Prf1 in mouse and human NK cells that represses NK cell lytic activity. PMID: 24698324
  19. Perforin and granzymes have complementary roles mediating epithelial injury by NK and CD8 T cells. The prevention of experimental biliary atresia can only be achieved by inhibiting both granules. PMID: 24096050
  20. Multiple roles of perforin in hampering ERBB-2 (Her-2/neu) carcinogenesis in transgenic male mice. PMID: 24790144
  21. perforin preferentially delivers cationic molecules while anionic and neutral cargoes are delivered inefficiently PMID: 24558045
  22. perforin's contribution to bacterial clearance in vivo is not though enhancing CD4 T cell termination of Chlamydia replication in epithelial cells PMID: 23691028
  23. These findings demonstrate that perforin-mediated immunoregulation functions in trans and are consistent with a feedback model in which cytotoxic T cells control immune activation by killing dendritic cells. PMID: 23974195
  24. These data suggest that perforin expression is not required for normal immune regulation. PMID: 23883515
  25. Distinct severity of HLH in both human and murine mutants with complete loss of cytotoxic effector PRF1, RAB27A, and STX11. PMID: 23160464
  26. Perforin facilitates viral antigen uptake by pulmonary dendritic cells by inducing apoptosis in mice with influenza infection. PMID: 22869906
  27. Perforin/FasL-independent functions of hapten-primed CD8 T cells in a contact hypersensitivity model identify new functions for neutrophils in regulating effector CD8 T cell recruitment and immune responses in the skin. PMID: 22815291
  28. deficiency of T-cells, NKT-cells, perforin, Fas-ligand, TNF-alpha-receptor failed to reveal significant differences in tumor development. PMID: 22212899
  29. CD8+ T-cells expressing interferon gamma or perforin play antagonistic roles in heart injury in experimental Trypanosoma cruzi-elicited cardiomyopathy PMID: 22532799
  30. CD4 cells expressing gamma interferon and perforin mediate protection against lethal influenza virus infection. PMID: 22491469
  31. A perforin-dependent pathwy plays a role to mediate contraction of antigen-specific CD8+ and CD4+ T cells during prolonged Listeria monocytogenes infection. PMID: 22161269
  32. Data show that transitional stage 1, 2 and splenic marginal zone B cells were clearly reduced after transfer of CD4+ T cells from TNFalpha-/-, IFNgamma-/-, perforin-/-, or FasLgld mice. PMID: 21966366
  33. Structures that PFN oligomers form in the membrane bilayer may include arcs previously observed by electron microscopy and these unusual structures represent an incomplete mixture of plasma membrane lipid. PMID: 21931672
  34. We conclude that perforin-dependent cytotoxicity has an immunoregulatory role that is distinguishable from its pathogen clearance function and limits T-cell activation in the physiologic context by suppressing antigen presentation. PMID: 21606480
  35. intact C terminus and N-linked glycosylation provide accurate and efficient export of perforin from the endoplasmic reticulum to the secretory granules and are critical for cytotoxic lymphocyte survival PMID: 21658975
  36. Data suggest that an interdependent relationship between parasite burden and CD8(+) T cells dictates the onset of perforin/GzmB-mediated ECM. PMID: 21525386
  37. Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence. PMID: 21304931
  38. At the Prf1 locus, clear areas of reduced nucleosomal density are detected in effector CD8-positive T cells surrounding the transcription start site but not in downstream areas of the genes, during lymphocytic choriomeningitis viral infection. PMID: 21278341
  39. Perforin was dispensable for efficient clearance of antigen-bearing cells from immunized mice, but only if CD95/CD95L was functional; however, there was a delay in target cell clearance in the absence of perforin. PMID: 20309009
  40. Granule-bound cathepsins are essential for processing perforin to its active form, and that CatL is an important, but not exclusive, participant in this process. PMID: 20497254
  41. elucidation of the mechanism of perforin pore formation by determining the X-ray crystal structure of monomeric murine perforin, together with a cryo-electron microscopy reconstruction of the entire perforin pore PMID: 21037563
  42. Perforin-mediated cytotoxicity by CD8 T cells is definitively responsible for muscle injury in C protein-induced myositis (CIM) PMID: 20583106
  43. Perforin deficiency attenuates inflammation and tumor growth in colitis-associated cancer. PMID: 19785028
  44. Pathogenetically relevant immune reactions in proteolipid protein-overexpressing mice are TCR-dependent and mediated by the classical components of CD8+ T-cell cytotoxicity, perforin, and Gzmb. PMID: 20042681
  45. role for perforin-, Fas/FasL-, and TNFR1-mediated cytotoxic pathways in down-regulation of antigen-specific T cells during persistent viral infection PMID: 11752172
  46. Using perforin-knockout (PKO) mice, we have seen that the granule exocytosis pathway can play a major role in NK cell-mediated rejection of allogeneic and MHC class I-deficient BMC, depending upon the genetics of the recipient and housing conditions. PMID: 11870623
  47. An intronic silencer of the mouse perforin gene. PMID: 11911476
  48. the genes for perforin, the three major T cell granzymes (A-C) and IFN-gamma are differentially expressed during primary activation of naive CD8(+) T cells, kinetically and at the single-cell level PMID: 12039912
  49. The immunodominance hierarchy of influenza virus-specific T(CD8+) was not greatly perturbed by the absence of either perforin or T-helper cells or by interference with B7 (CD80)-mediated signaling. PMID: 12239309
  50. Perforin protein expression is severely impaired in MEF-deficient NK cells PMID: 12387738

Show More

Hide All

Subcellular Location
Cytolytic granule. Secreted. Cell membrane; Multi-pass membrane protein. Endosome lumen.
Protein Families
Complement C6/C7/C8/C9 family
Tissue Specificity
Detected in cytotoxic T-lymphocytes and natural killer cells.
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
7505 Fannin St., Ste 610, Room 322 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join Us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2023 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1