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Perforin-1 is a protein in humans that is encoded by PRF1 gene. Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
The following Prf1 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
Prf1 Antibodies for Homo sapiens (Human)
| Code | Product Name | Species Reactivity | Application |
|---|---|---|---|
| CSB-PA018668GA01HU | PRF1 Antibody |
Human | ELISA,WB |
| CSB-PA018668LA01HU | PRF1 Antibody |
Human | ELISA |
| CSB-PA018668LC01HU | PRF1 Antibody, FITC conjugated |
Human | |
| CSB-PA018668LD01HU | PRF1 Antibody, Biotin conjugated |
Human | ELISA |
Prf1 Antibodies for Mus musculus (Mouse)
| Code | Product Name | Species Reactivity | Application |
|---|---|---|---|
| CSB-PA018668LA01MO | Prf1 Antibody |
Mouse | ELISA |
| CSB-PA018668LB01MO | Prf1 Antibody, HRP conjugated |
Mouse | ELISA |
| CSB-PA018668LC01MO | Prf1 Antibody, FITC conjugated |
Mouse | |
| CSB-PA018668LD01MO | Prf1 Antibody, Biotin conjugated |
Mouse | ELISA |
| CSB-PA018668ZA01MO | Prf1 Antibody |
Mus musculus | ELISA, WB (ensure identification of antigen) |
Prf1 Antibodies for Rattus norvegicus (Rat)
| Code | Product Name | Species Reactivity | Application |
|---|---|---|---|
| CSB-PA018668ZA01RA | Prf1 Antibody |
Rattus norvegicus | ELISA, WB (ensure identification of antigen) |
Prf1 Proteins for Mus musculus (Mouse)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP018668MO | Recombinant Mouse Perforin-1 (PRF1) |
Yeast |
| CSB-EP018668MO | Recombinant Mouse Perforin-1 (Prf1) |
E.coli |
| CSB-BP018668MO CSB-MP018668MO CSB-EP018668MO-B |
Recombinant Mouse Perforin-1 (Prf1) |
Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Prf1 Proteins for Homo sapiens (Human)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP018668HU CSB-MP018668HU CSB-EP018668HU-B |
Recombinant Human Perforin-1 (PRF1) |
Yeast Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-EP018668HU | Recombinant Human Perforin-1 (PRF1) |
E.coli |
| CSB-BP018668HU | Recombinant Human Perforin-1 (PRF1) |
Baculovirus |
Prf1 Proteins for Rattus norvegicus (Rat)
| Code | Product Name | Source |
|---|---|---|
| CSB-EP018668RA | Recombinant Rat Perforin-1 (Prf1), partial |
E.coli |
| CSB-YP018668RA CSB-BP018668RA CSB-MP018668RA CSB-EP018668RA-B |
Recombinant Rat Perforin-1 (PRF1), partial |
Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Prf1 Proteins for Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP349938TDL CSB-EP349938TDL CSB-BP349938TDL CSB-MP349938TDL CSB-EP349938TDL-B |
Recombinant Thermoplasma volcanium Peptide chain release factor subunit 1 (prf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Prf1 Proteins for Methanococcus maripaludis (strain S2 / LL)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP351670MSB CSB-EP351670MSB CSB-BP351670MSB CSB-MP351670MSB CSB-EP351670MSB-B |
Recombinant Methanococcus maripaludis Peptide chain release factor subunit 1 (prf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Prf1 Proteins for Methanococcus maripaludis (strain C5 / ATCC BAA-1333)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP389400MNP CSB-EP389400MNP CSB-BP389400MNP CSB-MP389400MNP CSB-EP389400MNP-B |
Recombinant Methanococcus maripaludis Peptide chain release factor subunit 1 (prf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Prf1 Proteins for Methanobrevibacter smithii (strain ATCC 35061 / DSM 861 / OCM 144 / PS)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP404375MNN CSB-EP404375MNN CSB-BP404375MNN CSB-MP404375MNN CSB-EP404375MNN-B |
Recombinant Methanobrevibacter smithii Peptide chain release factor subunit 1 (prf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Prf1 Proteins for Methanococcus vannielii (strain ATCC 35089 / DSM 1224 / JCM 13029 / OCM 148 / SB)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP407775MNS CSB-EP407775MNS CSB-BP407775MNS CSB-MP407775MNS CSB-EP407775MNS-B |
Recombinant Methanococcus vannielii Peptide chain release factor subunit 1 (prf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Prf1 Proteins for Methanococcus maripaludis (strain C7 / ATCC BAA-1331)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP411969MNR CSB-EP411969MNR CSB-BP411969MNR CSB-MP411969MNR CSB-EP411969MNR-B |
Recombinant Methanococcus maripaludis Peptide chain release factor subunit 1 (prf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Prf1 Proteins for Methanococcus maripaludis (strain C6 / ATCC BAA-1332)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP429897MNQ CSB-EP429897MNQ CSB-BP429897MNQ CSB-MP429897MNQ CSB-EP429897MNQ-B |
Recombinant Methanococcus maripaludis Peptide chain release factor subunit 1 (prf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Prf1 Proteins for Thermococcus onnurineus (strain NA1)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP469657TMR CSB-EP469657TMR CSB-BP469657TMR CSB-MP469657TMR CSB-EP469657TMR-B |
Recombinant Thermococcus onnurineus Peptide chain release factor subunit 1 (prf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Prf1 Proteins for Halorubrum lacusprofundi (strain ATCC 49239 / DSM 5036 / JCM 8891 / ACAM 34)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP498604HUB CSB-EP498604HUB CSB-BP498604HUB CSB-MP498604HUB CSB-EP498604HUB-B |
Recombinant Halorubrum lacusprofundi Peptide chain release factor subunit 1 (prf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Prf1 Proteins for Thermococcus sibiricus (strain MM 739 / DSM 12597)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP509588TMT CSB-EP509588TMT CSB-BP509588TMT CSB-MP509588TMT CSB-EP509588TMT-B |
Recombinant Thermococcus sibiricus Peptide chain release factor subunit 1 (prf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Prf1 Proteins for Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP518099DOC CSB-EP518099DOC CSB-BP518099DOC CSB-MP518099DOC CSB-EP518099DOC-B |
Recombinant Archaeoglobus fulgidus Peptide chain release factor subunit 1 (prf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Prf1 Proteins for Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP521420MSR CSB-EP521420MSR CSB-BP521420MSR CSB-MP521420MSR CSB-EP521420MSR-B |
Recombinant Methanothermobacter thermautotrophicus Peptide chain release factor subunit 1 (prf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Prf1 Proteins for Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP523929FHX CSB-EP523929FHX CSB-BP523929FHX CSB-MP523929FHX CSB-EP523929FHX-B |
Recombinant Pyrococcus horikoshii Peptide chain release factor subunit 1 (prf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Prf1 Proteins for Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP532255HTL CSB-EP532255HTL CSB-BP532255HTL CSB-MP532255HTL CSB-EP532255HTL-B |
Recombinant Halobacterium salinarum Peptide chain release factor subunit 1 (prf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Prf1 Proteins for Methanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP618728MRY CSB-EP618728MRY CSB-BP618728MRY CSB-MP618728MRY CSB-EP618728MRY-B |
Recombinant Methanococcoides burtonii Peptide chain release factor subunit 1 (prf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Prf1 Proteins for Haloquadratum walsbyi (strain DSM 16790 / HBSQ001)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP621145HAAE CSB-EP621145HAAE CSB-BP621145HAAE CSB-MP621145HAAE CSB-EP621145HAAE-B |
Recombinant Haloquadratum walsbyi Peptide chain release factor subunit 1 (prf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Prf1 Proteins for Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP646720MAAR CSB-EP646720MAAR CSB-BP646720MAAR CSB-MP646720MAAR CSB-EP646720MAAR-B |
Recombinant Methanosphaera stadtmanae Peptide chain release factor subunit 1 (prf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Prf1 Proteins for Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
| Code | Product Name | Source |
|---|---|---|
| CSB-YP686349FHY CSB-EP686349FHY CSB-BP686349FHY CSB-MP686349FHY CSB-EP686349FHY-B |
Recombinant Pyrococcus kodakaraensis Peptide chain release factor subunit 1 (prf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Prf1 Proteins for Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP688247MRU CSB-EP688247MRU CSB-BP688247MRU CSB-MP688247MRU CSB-EP688247MRU-B |
Recombinant Methanocaldococcus jannaschii Peptide chain release factor subunit 1 (prf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Prf1 Proteins for Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP713307HTJ CSB-EP713307HTJ CSB-BP713307HTJ CSB-MP713307HTJ CSB-EP713307HTJ-B |
Recombinant Haloarcula marismortui Peptide chain release factor subunit 1 (prf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Prf1 Proteins for Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP740311PAAB CSB-EP740311PAAB CSB-BP740311PAAB CSB-MP740311PAAB CSB-EP740311PAAB-B |
Recombinant Picrophilus torridus Peptide chain release factor subunit 1 (prf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Prf1 Proteins for Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP819108MSN CSB-EP819108MSN CSB-BP819108MSN CSB-MP819108MSN CSB-EP819108MSN-B |
Recombinant Methanosarcina mazei Peptide chain release factor subunit 1 (prf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Prf1 Proteins for Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP837591FHW CSB-EP837591FHW CSB-BP837591FHW CSB-MP837591FHW CSB-EP837591FHW-B |
Recombinant Pyrococcus furiosus Peptide chain release factor subunit 1 (prf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Prf1 Proteins for Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP841864FHU CSB-EP841864FHU CSB-BP841864FHU CSB-MP841864FHU CSB-EP841864FHU-B |
Recombinant Pyrobaculum aerophilum Peptide chain release factor subunit 1 (prf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Prf1 Proteins for Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP842955FPM CSB-EP842955FPM CSB-BP842955FPM CSB-MP842955FPM CSB-EP842955FPM-B |
Recombinant Sulfolobus solfataricus Peptide chain release factor subunit 1 (prf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Prf1 Proteins for Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP848521MSI CSB-EP848521MSI CSB-BP848521MSI CSB-MP848521MSI CSB-EP848521MSI-B |
Recombinant Methanopyrus kandleri Peptide chain release factor subunit 1 (prf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Prf1 Proteins for Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP881086TND CSB-EP881086TND CSB-BP881086TND CSB-MP881086TND CSB-EP881086TND-B |
Recombinant Thermoplasma acidophilum Peptide chain release factor subunit 1 (prf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Prf1 Proteins for Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP888112HTM CSB-EP888112HTM CSB-BP888112HTM CSB-MP888112HTM CSB-EP888112HTM-B |
Recombinant Halobacterium salinarum Peptide chain release factor subunit 1 (prf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Prf1 Proteins for Pyrococcus abyssi (strain GE5 / Orsay)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP896010FHV CSB-EP896010FHV CSB-BP896010FHV CSB-MP896010FHV CSB-EP896010FHV-B |
Recombinant Pyrococcus abyssi Peptide chain release factor subunit 1 (prf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Prf1 ELISA Kit for Homo sapiens (Human)
| Code | Product Name | Sample Types | Sensitivity |
|---|---|---|---|
| CSB-E09313h | Human Perforin-1 (PRF1/PFP) ELISA kit |
serum, plasma, tissue homogenates | 0.078 ng/mL |
Prf1 ELISA Kit for Mus musculus (Mouse)
| Code | Product Name | Sample Types | Sensitivity |
|---|---|---|---|
| CSB-E13429m | Mouse Perforin/Pore-forming protein,PF/PFP ELISA Kit |
serum, plasma, cell culture supernates | 1.95 pg/mL |
The PRF1 gene encodes perforin[1][2]. Perforin is found in immune cells (lymphocytes) called T cells and natural killer (NK) cells, which destroy other cells. Perforin is involved in the process of cell destruction (cytolysis) and the regulation of the immune system. Perforin is a major component of structures called cytolytic granules within T cells and NK cells. One of the main ways in which T cells and NK cells destroy other cells is to transport and secrete these cytolytic granules, which contain cell-killing proteins, onto the membranes of the target cells.
Perforin helps create a channel through the membrane, allowing cytolytic proteins to enter the cell and trigger it to self-destruct. This cytolytic mechanism also helps regulate the immune system by destroying unneeded T cells. Controlling the number of T cells prevents the overproduction of immune proteins called cytokines that lead to inflammation and, in excess, cause tissue damage. It also can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Besides, It promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
[1] Fink TM, Zimmer M, Weitz S, Tschopp J, Jenne DE, Lichter P (Sep 1992). "Human perforin (PRF1) maps to 10q22, a region that is syntenic with mouse chromosome 10". Genomics. 13 (4): 1300–2.
[2] Shinkai Y, Yoshida MC, Maeda K, Kobata T, Maruyama K, Yodoi J, Yagita H, Okumura K (Jan 1990). "Molecular cloning and chromosomal assignment of a human perforin (PFP) gene". Immunogenetics. 30 (6): 452–7.
