Mouse Platelet Factor 4,PF-4 ELISA Kit

Code CSB-E07884m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


The Mouse Platelet Factor 4 (PF4) ELISA Kit allows for the detection and quantification of PF4 in mouse serum, plasma, or tissue homogenates. This kit has high sensitivity, excellent specificity, precision less than 10%, high recovery, good linearity, and consistency between batches. It employs the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction to achieve the detection. The solution color develops in proportion to the amount of PF4 in the sample, and the color intensity can be measured at 450 nm via a microplate reader.

PF4 is a positively charged tetramer belonging to the CXC chemokine family. It is released from the alpha-granules of activated platelets. PF4 inhibits local antithrombin III activity by binding with high affinity to heparin-like molecules thus promoting coagulation. It also participates in many other biological processes, including promoting the survival of hematopoietic stem cells and suppressing the proliferation of endothelial cells and fibroblasts. PF4 has recently demonstrated to form stable heterodimers with CCL5, leading to a synergistically amplified ability to recruit monocytes. In the terms of inflammation, PF4 promotes the adherence of neutrophils onto endothelial cells, facilitates neutrophil exocytosis, and supports the generation of reactive oxygen species and other pro-inflammatory cytokines from monocytes to maintain the immune response. The binding of pathogenic antibodies to PF4/heparin complex and subsequent activation of cellular FcγRIIA on platelets and monocytes, resulting in life-threatening thrombosis that causes heparin-induced thrombocytopenia (HIT).

Target Name platelet factor 4
Alternative Names Pf4 ELISA Kit; Cxcl4 ELISA Kit; Scyb4 ELISA Kit; Platelet factor 4 ELISA Kit; PF-4 ELISA Kit; C-X-C motif chemokine 4 ELISA Kit
Abbreviation PF4
Uniprot No. Q9Z126
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.125 ng/mL-200 ng/mL
Sensitivity 1.603 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PF-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:200 Average % 86
Range % 81-90
1:400 Average % 98
Range % 92-104
1:800 Average % 97
Range % 94-102
1:1600 Average % 94
Range % 89-98
The recovery of mouse PF-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 89 86-94
EDTA plasma (n=4) 98 92-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
200 2.501 2.402 2.452 2.308
100 2.093 1.975 2.034 1.890
50 1.354 1.383 1.369 1.225
25 0.778 0.769 0.774 0.630
12.5 0.386 0.371 0.379 0.235
6.25 0.268 0.257 0.263 0.119
3.125 0.209 0.202 0.206 0.062
0 0.144 0.143 0.144  
Materials provided
  • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse PF4 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled PF4 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
  • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
  • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation (By similarity).
Gene References into Functions
  1. these data demonstrate that PF4 has an important role in increasing B cell differentiation in the bone marrow environment PMID: 28914425
  2. these results identify CXCL4 as a vital immunoregulatory chemokine essential for protecting mice against influenza A virus infection, especially as it affects the development of lung injury and neutrophil mobilization to the inflamed lung PMID: 28120850
  3. These results indicate that CXCL4 is a novel Ni-binding protein that augments Ni allergy at the elicitation and sensitization phases. This is the first study to demonstrate that the Ni-binding protein augments Ni allergy in vivo. PMID: 28319310
  4. PF4 was produced by Ly6G+CD11b+ immature myeloid cells in the early stage premetastatic lungs, and decreased during metastatic progression. PMID: 27223426
  5. These data demonstrate that the CXCR2 network and CXCL4 play a role in the maintenance of normal HSC/HPC cell fates, including survival and self-renewal. PMID: 27222476
  6. Platelet-derived CXCL7 and CXCL4 contribute to the pathogenesis of acute lung injury. PMID: 27755915
  7. CXCL4 plays an important role in pancreatic inflammation PMID: 27183218
  8. Platelet secretion of CXCL4 is Rac1-dependent and regulates neutrophil infiltration and tissue damage in septic lung damage PMID: 26478565
  9. PF4 has a complex intramedullary life cycle with important implications in megakaryopoiesis and hematopoietic stem cell replication not seen with other tested alpha granule proteins. PMID: 26256688
  10. Heparin enhances antigen uptake and activation of the initial steps in the cellular immune response to PF4-containing complexes. PMID: 25960020
  11. Data indicate that platelet factor 4 (PF4) is involved directly in liver innate immune response against ischaemia-reperfusion injury (IRI) by regulating Th17 cell differentiation. PMID: 25440775
  12. CXCL4 regulates hematopoietic stem cell cell cycle activity. PMID: 25326802
  13. Platelet factor 4 has a role in regulating Th17 differentiation and cardiac allograft rejection PMID: 24463452
  14. Histones regulate activated protein C formation in a manner similar to PF4 and suggest heparinoids may be benificial in sepsis. PMID: 24177324
  15. PF4 drives a vascular smooth muscle inflammatory phenotype including a decline in differentiation markers, increased cytokine production, and cell proliferation. PMID: 23568488
  16. PF4 expression on intestinal epithelial cells is increased after IR at both the mRNA and protein levels. In conclusion, these findings demonstrate that may PF4 represent an important mediator of local and remote tissue damage. PMID: 22792197
  17. These results suggest that Cxcl4 and Cxcl7 play a role in the development of neurobehavioral alterations that are triggered by in utero TCDD exposure and later surface in adults. PMID: 21509788
  18. PF4 may have a role in bacterial defense, and heparin-induced thrombocytopenia is probably a misdirected antibacterial host defense mechanism. PMID: 20959601
  19. In vivo, half-life and diffusibility of CXCL4L1 compared with Cxcl4 is strongly increased. PMID: 20688960
  20. These data indicate that PF4 induction of monocyte KLF4 expression may be an important step in the pathogenesis of experimental cerebral malaria. PMID: 20454664
  21. Micronutrients effectively promote tumor dormancy in early prostate cancer by inducing platelet factor-4 expression and concentrating it at the tumor endothelium through enhanced platelet binding. PMID: 20525356
  22. CXC chemokine ligand 4 (Cxcl4) is a platelet-derived mediator of experimental liver fibrosis. PMID: 20162727
  23. important role for PF4 in thrombosis and neutralization of PF4 is an important component of heparin's anticoagulant effect. PMID: 14764524
  24. Platelet factor 4 and interleukin-8 CXC chemokine heterodimer formation modulates function at the quaternary structural level PMID: 15531763
  25. the PF4 storage pathway in alpha-granules is not a default pathway, but rather a regular granule storage pathway probably requiring specific sorting mechanisms PMID: 15613031
  26. analysis of granule targeting sequence within platelet factor 4 PMID: 15964840
  27. clinical heterogeneity in the HIT immune response may be due in part to requirements for specific biophysical parameters of the PF4/heparin complexes that occur in settings of intense platelet activation and PF4 release PMID: 17848616
  28. platelet-associated PF-4, but not its plasma counterpart, may represent a potential biomarker of early tumor presence PMID: 17914028
  29. platelet specific chemokine PF4 promotes atherosclerotic lesion development in vivo PMID: 18000617
  30. Brain microglia are a cellular source of CXCL4 gene expression. PMID: 18248618
  31. platelet-derived PF4 contributes to immune activation and T cell trafficking as part of the pathogenesis of experimental cerebral malaria . PMID: 18692777
  32. Platelet factor 4 regulates megakaryopoiesis through low-density lipoprotein receptor-related protein 1 (LRP1) on megakaryocytes. PMID: 19605848

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Subcellular Location Secreted
Protein Families Intercrine alpha (chemokine CxC) family
Database Links

KEGG: mmu:56744

STRING: 10090.ENSMUSP00000031320

UniGene: Mm.332490


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