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24T ELISA Kit Trial Size (Only USD$150/ kit)
|Intra-assay Precision (Precision within an assay): CV%<8%|
|Three samples of known concentration were tested twenty times on one plate to assess.|
|Inter-assay Precision (Precision between assays): CV%<10%|
|Three samples of known concentration were tested in twenty assays to assess.|
|To assess the linearity of the assay, samples were spiked with high concentrations of mouse cTn-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.|
|The recovery of mouse cTn-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.|
|Sample Type||Average % Recovery||Range|
|EDTA plasma (n=4)||92||88-96|
|These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.|
The mouse cTnI ELISA Kit quantitates mouse cTnI levels in serum and plasma. cTnI (TNNI3) is uniquely expressed in cardiac sarcomeres. It is the inhibitory component of the heterotrimeric troponin complex (cTn) that controls calcium ion-dependent activation of sarcomeres. cTnI functions as a key modulatory protein in cardiac muscle contraction and relaxation, combining calcium ion-troponin C (TnC) binding with activation of cross-bridge reactions with the thin filament. Increased serum levels of cTnI are identified as highly sensitive and specific indicators of myocardial injury. Serial determination of cTnI is routinely applied in the evaluation of patients with the acute coronary syndrome (ACS) for diagnosis and prognosis.
This kit employs the sandwich-ELISA mechanism in conjugation with cTnI antibody-cTnI antigen-specific binding as well as HRP-TMB chromogenic reaction to measure the concentration of cTnI in the samples. The kit is characterized by high sensitivity, strong specificity, good linearity, high recovery, and a precision of less than 10%.
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