Pig Endothelial nitric oxide synthase,eNOS ELISA Kit

Code CSB-E08574p
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Product Details

Target Name
nitric oxide synthase 3 (endothelial cell)
Alternative Names
NOS3 ELISA Kit; NOS ELISA Kit; Nitric oxide synthase ELISA Kit; endothelial ELISA Kit; EC ELISA Kit; Constitutive NOS ELISA Kit; cNOS ELISA Kit; EC-NOS ELISA Kit; Endothelial NOS ELISA Kit; eNOS ELISA Kit; NOS type III ELISA Kit; NOSIII ELISA Kit
Uniprot No.
Sus scrofa (Pig)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
3.12 IU/mL-200 IU/mL
0.78 IU/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of pig eNOS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 83  
Range % 80-86  
1:2 Average % 97  
Range % 93-100  
1:4 Average % 84  
Range % 80-88  
1:8 Average % 104  
Range % 100-108  
The recovery of pig eNOS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 85-92  
EDTA plasma (n=4) 100 97-103  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
IU/ml OD1 OD2 Average Corrected  
200 2.589 2.547 2.568 2.386  
100 1.829 1.809 1.819 1.637  
50 1.064 1.087 1.076 0.894  
25 0.643 0.657 0.650 0.468  
12.5 0.450 0.468 0.459 0.277  
6.25 0.358 0.369 0.364 0.182  
3.12 0.274 0.298 0.286 0.104  
0 0.181 0.183 0.182    
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Pig NOS3 ELISA Kit was designed for the quantitative measurement of Pig NOS3 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 3.12 IU/mL-200 IU/mL and the sensitivity is 0.78 IU/mL.

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Target Background

(From Uniprot)
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Gene References into Functions
  1. NOS3 was lowest in kidneys removed from live pigs, greater in kidneys from pigs with brain death, and greatest in kidneys from pigs with cardiac arrest. PMID: 25380860
  2. Rapid atrial pacing increases ADMA and down-regulates eNOS expression in an ADMA-independent manner. PMID: 20926145
  3. Icariin and icariside II may increase the eNOS expression through activating EGF-EGFR pathway in porcine aortic endothelial cells. PMID: 21844953
  4. Data suggest that pig sperm contain bNOS, iNOS, and eNOS; up-regulation of NOS by leptin during acrosome reaction and inhibition of acrosome reaction by inhibitors of nitric oxide synthases suggests these enzymes are involved in acrosome reaction. PMID: 21970701
  5. Periodic acceleration (pGz) acutely increases endothelial and neuronal nitric oxide synthase expression in endomyocardium of normal swine. PMID: 19022311
  6. Data suggest that angiotensin II regulates nNOS and eNOS expression and NOS activity in afferent arterioles of the developing kidney via angiotensin 1 and 2 receptors. PMID: 20386492
  7. Endothelial nitric oxide synthase mRNA expression was elevated in gestational day 50 intrauterine growth retardation placenta and areola compared to gd50 control. PMID: 20381143
  8. Oligonol prevented the impairment of eNOS activity induced by high glucose through reversing altered eNOS phosphorylation status. PMID: 20128797
  9. Exercise training significantly increased total eNOS and phosphorylated levels of eNOS (pSer(1179)) in collateral-dependent arteries of experimental minipigs. PMID: 20363881
  10. Data show that wall shear stress increases with a decrease in artery diameter; eNOS protein contents decrease with an increase in diameter. PMID: 19432590
  11. The inhibitory domain of eNOs stimulates vasodilation of the pulmonary artery. PMID: 14729513
  12. endothelium-derived NO stimulates guanylyl cyclase in vascular smooth muscle cells and, thereby, permits CO to cause dilation by activating K(Ca) channels PMID: 15191891
  13. the p38 MAPK is an upstream component of Akt-mediated eNOS activation PMID: 15333638
  14. Ritonavir significantly impairs vasomotor function and reduces eNOS expression in porcine coronary artery endothelial cells. PMID: 15905730
  15. AMPK activity is essential for estradiol-induced eNOS activation in vascular endothelial cells via the promotion of eNOS interaction with HSP90 PMID: 15967841
  16. Hsp90 is a modulator of eNOS activity and vascular reactivity in the newborn piglet pulmonary circulation PMID: 17337508
  17. Glomerular eNOS gene expression was studied during postnatal maturation and AT1 receptor inhibition. PMID: 17437130
  18. in the aortic arch, nitric oxide synthase 3 endothelial (eNOS) was downregulated, compared with the thoracic aorta PMID: 17588585
  19. eNOS phosphorylation at Ser-116, therefore, appears to contribute to negative modulation of eNOS activity and hence to regulation of vascular tone. PMID: 17822962
  20. Analysis of the 2.1 kb 5' flanking region reveals that the swine eNOS promoter is, like its counterparts in human and other species, a TATA-less promoter; the proximal region from -227 to -82 is necessary for eNOS basal promoter activity. PMID: 17852356
  21. regenerated coronary endothelial cells exhibit downregulation of eNOS PMID: 17942849
  22. Immunoreactivity of eNOS was similar to NADPH-d staining. Clear iNOS immunoreactivity was detected in the luminal epithelium, endometrial stroma and individual endometrial glands. PMID: 18165177
  23. Low-fat diet and chronic exercise preserve endothelial function in the pig penis by sustaining active eNOS in its dimeric form and by limiting eNOS interaction with its negative regulator caveolin-1. PMID: 18194184
  24. Effects of chronic NOS3 inhibition on endothelium-dependent and -independent relaxation in arteries that perfuse skeletal muscle of swine are reported. PMID: 18568942
  25. The increase in NOS protein seen in both the endothelium and vascular smooth muscle in response to cerebral vasospasm is enhanced by dopamine in a D(2)R-dependent mechanism PMID: 18807216
  26. results suggest that endothelial (eNOS) and inducible (iNOS) nitric oxide synthase are responsible for regulation of blood circulation in umbilical cord PMID: 18992077
  27. Report effect of uncoupling endothelial nitric oxide synthase on calcium homeostasis in aged porcine endothelial cells. PMID: 19176602
  28. Low expressions of eNOS3 and Ve-cadherin in the salvaged sub-healthy microvascular endothelium of infarcted and marginal areas suggest that endothelial system is impaired at 7-day of reperfused acute myocardial infarction. PMID: 19519369
  29. ENOS activation during cardioplegic arrest occurs through phosphorylation at Ser1177 mediated by Akt/PKB. PMID: 19795322

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Subcellular Location
Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Cell membrane.
Protein Families
NOS family
Database Links
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