Recombinant Pig Nitric oxide synthase, endothelial (NOS3), partial

Code CSB-YP637927PI
MSDS
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Source Yeast
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Code CSB-EP637927PI
MSDS
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Source E.coli
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Code CSB-EP637927PI-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP637927PI
MSDS
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Source Baculovirus
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Code CSB-MP637927PI
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NOS3
Uniprot No.
Alternative Names
NOS3; NOS; Nitric oxide synthase; endothelial; EC 1.14.13.39; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII
Species
Sus scrofa (Pig)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Gene References into Functions
  1. NOS3 was lowest in kidneys removed from live pigs, greater in kidneys from pigs with brain death, and greatest in kidneys from pigs with cardiac arrest. PMID: 25380860
  2. Rapid atrial pacing increases ADMA and down-regulates eNOS expression in an ADMA-independent manner. PMID: 20926145
  3. Icariin and icariside II may increase the eNOS expression through activating EGF-EGFR pathway in porcine aortic endothelial cells. PMID: 21844953
  4. Data suggest that pig sperm contain bNOS, iNOS, and eNOS; up-regulation of NOS by leptin during acrosome reaction and inhibition of acrosome reaction by inhibitors of nitric oxide synthases suggests these enzymes are involved in acrosome reaction. PMID: 21970701
  5. Periodic acceleration (pGz) acutely increases endothelial and neuronal nitric oxide synthase expression in endomyocardium of normal swine. PMID: 19022311
  6. Data suggest that angiotensin II regulates nNOS and eNOS expression and NOS activity in afferent arterioles of the developing kidney via angiotensin 1 and 2 receptors. PMID: 20386492
  7. Endothelial nitric oxide synthase mRNA expression was elevated in gestational day 50 intrauterine growth retardation placenta and areola compared to gd50 control. PMID: 20381143
  8. Oligonol prevented the impairment of eNOS activity induced by high glucose through reversing altered eNOS phosphorylation status. PMID: 20128797
  9. Exercise training significantly increased total eNOS and phosphorylated levels of eNOS (pSer(1179)) in collateral-dependent arteries of experimental minipigs. PMID: 20363881
  10. Data show that wall shear stress increases with a decrease in artery diameter; eNOS protein contents decrease with an increase in diameter. PMID: 19432590
  11. The inhibitory domain of eNOs stimulates vasodilation of the pulmonary artery. PMID: 14729513
  12. endothelium-derived NO stimulates guanylyl cyclase in vascular smooth muscle cells and, thereby, permits CO to cause dilation by activating K(Ca) channels PMID: 15191891
  13. the p38 MAPK is an upstream component of Akt-mediated eNOS activation PMID: 15333638
  14. Ritonavir significantly impairs vasomotor function and reduces eNOS expression in porcine coronary artery endothelial cells. PMID: 15905730
  15. AMPK activity is essential for estradiol-induced eNOS activation in vascular endothelial cells via the promotion of eNOS interaction with HSP90 PMID: 15967841
  16. Hsp90 is a modulator of eNOS activity and vascular reactivity in the newborn piglet pulmonary circulation PMID: 17337508
  17. Glomerular eNOS gene expression was studied during postnatal maturation and AT1 receptor inhibition. PMID: 17437130
  18. in the aortic arch, nitric oxide synthase 3 endothelial (eNOS) was downregulated, compared with the thoracic aorta PMID: 17588585
  19. eNOS phosphorylation at Ser-116, therefore, appears to contribute to negative modulation of eNOS activity and hence to regulation of vascular tone. PMID: 17822962
  20. Analysis of the 2.1 kb 5' flanking region reveals that the swine eNOS promoter is, like its counterparts in human and other species, a TATA-less promoter; the proximal region from -227 to -82 is necessary for eNOS basal promoter activity. PMID: 17852356
  21. regenerated coronary endothelial cells exhibit downregulation of eNOS PMID: 17942849
  22. Immunoreactivity of eNOS was similar to NADPH-d staining. Clear iNOS immunoreactivity was detected in the luminal epithelium, endometrial stroma and individual endometrial glands. PMID: 18165177
  23. Low-fat diet and chronic exercise preserve endothelial function in the pig penis by sustaining active eNOS in its dimeric form and by limiting eNOS interaction with its negative regulator caveolin-1. PMID: 18194184
  24. Effects of chronic NOS3 inhibition on endothelium-dependent and -independent relaxation in arteries that perfuse skeletal muscle of swine are reported. PMID: 18568942
  25. The increase in NOS protein seen in both the endothelium and vascular smooth muscle in response to cerebral vasospasm is enhanced by dopamine in a D(2)R-dependent mechanism PMID: 18807216
  26. results suggest that endothelial (eNOS) and inducible (iNOS) nitric oxide synthase are responsible for regulation of blood circulation in umbilical cord PMID: 18992077
  27. Report effect of uncoupling endothelial nitric oxide synthase on calcium homeostasis in aged porcine endothelial cells. PMID: 19176602
  28. Low expressions of eNOS3 and Ve-cadherin in the salvaged sub-healthy microvascular endothelium of infarcted and marginal areas suggest that endothelial system is impaired at 7-day of reperfused acute myocardial infarction. PMID: 19519369
  29. ENOS activation during cardioplegic arrest occurs through phosphorylation at Ser1177 mediated by Akt/PKB. PMID: 19795322

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Subcellular Location
Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Cell membrane.
Protein Families
NOS family
Database Links
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