Pig Interferon α,IFN-α ELISA Kit

Code CSB-E07328p
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Interferon α,IFN-α
Abbreviation IFNA1
Uniprot No. Q6VAB8
Species Sus scrofa (Pig)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of pig IFN-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %89
Range %80-99
1:2Average %98
Range %91-105
1:4Average %96
Range %92-105
1:8Average %94
Range %86-98
The recovery of pig IFN-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-102
EDTA plasma (n=4)9790-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
5002.895 2.852 2.874 2.739
2502.114 2.117 2.116 1.981
1251.254 1.260 1.257 1.122
62.50.846 0.839 0.843 0.708
31.20.489 0.454 0.472 0.337
15.60.301 0.319 0.310 0.175
7.80.257 0.249 0.253 0.118
00.133 0.137 0.135  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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