Pig tissue inhibitors of metalloproteinase 1,TIMP-1 ELISA Kit

Code CSB-E06799p
Size 96T,5×96T,10×96T
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Product Details

Target Name
TIMP metallopeptidase inhibitor 1
Alternative Names
TIMP1 ELISA Kit; Metalloproteinase inhibitor 1 ELISA Kit; Tissue inhibitor of metalloproteinases 1 ELISA Kit; TIMP-1 ELISA Kit
Abbreviation
TIMP1
Uniprot No.
Species
Sus scrofa (Pig)
Sample Types
serum, plasma, tissue homogenates
Detection Range
7.8 ng/mL-500 ng/mL
Sensitivity
1.95 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig TIMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %93
Range %89-101
1:2Average %89
Range %83-98
1:4Average %92
Range %86-105
1:8Average %94
Range %89-107
Recovery
The recovery of pig TIMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9485-99
EDTA plasma (n=4)9792-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
5002.422 2.395 2.409 2.236
2501.466 1.506 1.486 1.313
1250.986 1.007 0.997 0.824
62.50.611 0.589 0.600 0.427
31.20.425 0.454 0.440 0.267
15.60.342 0.362 0.352 0.179
7.80.242 0.250 0.246 0.073
00.175 0.170 0.173
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Pig TIMP1 ELISA Kit was designed for the quantitative measurement of Pig TIMP1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 ng/mL-500 ng/mL and the sensitivity is 1.95 ng/mL.

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 Q&A
Q:

Regarding your ELISA kit CSB-E06799p, could you please advise on the sample types that you lab has tested with this kit, and what the observed concentration values were?
I was hoping to use their porcine cartilage homogenate samples with the kit. I have already homogenized the samples in RIPA buffer, and I was hoping you might be able to advise whether or not you thought RIPA might be a suitable lysis buffer for my samples?

A:
Thanks for your inquiry.
CSB-E06799p
We tested undiluted serum and plasma samples before and the test value is ND--30ng/ml. Pls find attached QC.
For cartilage samples, sorry we didn't use RIPA buffer before. Usually we use pretreatment combined with repeated freezing and thawing method.
Generally, RIPA will contain components such as SDS, Triton X-100, and NP-40. Pls note:
1. It must not contain SDS (which will destroy the hydrogen bond of protein macromolecules causing protein denaturation);
2. For Triton X-100, NP 40 and other mild detergents, you should control the low concentration range (generally no more than 1%, but it not verified, so we are not clear about the specific impact);
3.EGTA and EDTA can be used as chelating agents for metal ions to remove metal ions, which has a great influence on BCA method, but we are not clear about the impact on elisa;
4 The sample should not contain NaN3 components, which will inhibit HRP activity and cause false negative.
Therefore, if your sample has been prepared, you can do a pretest first but we are not clear about the final test result; if not prepared, it is recommended to follow the recommended method in our manual.
Pls let me know if you have any further questions. Thank you.

Target Background

Function
(From Uniprot)
Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling.
Gene References into Functions
  1. Results provide evidence for the utility of MMP9 and TIMP1 as markers of age- and lactocrine-sensitive porcine female reproductive tract development. PMID: 27866058
  2. we demonstrated the presence of high molecular weight (HMW) complexes (130, 170, and 220 kDa) containing MMP9, TIMP1, and NGAL (also MMP2 in 220 kDa complex) without proteolytic activity. PMID: 23710440
  3. Hemodialysis graft placement leads to early increases in wall shear stress, VEGF-A, pro-MMP-9, MMP-2, VEGFR-1, VEGFR-2, and TIMP-1, which may contribute to the development of venous stenosis. PMID: 18326810
  4. Results indicate that leukemia inhibitory factor (LIF) and Oncostatin M increase the expression of MMP-1, MMP-3, and TIMP-1 several fold, and that their expression is reduced to basal levels in the presence of the LIF antagonist MH35-BD. PMID: 19342253
Subcellular Location
Secreted.
Protein Families
Protease inhibitor I35 (TIMP) family
Database Links
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