Rat Arginase 1(ARG1) ELISA Kit

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Target Background

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Product Details

Description

This Rat ARG1 ELISA Kit was designed for the quantitative measurement of Rat ARG1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

Target Name

Arginase 1(ARG1)

Alternative Names

Arg1Arginase-1 ELISA kit; EC 3.5.3.1 ELISA kit; Liver-type arginase ELISA kit; Type I arginase ELISA kit

Abbreviation

ARG1

Uniprot No.

P07824

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat ARG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	89-101
1:2	Average %	98
	Range %	91-102
1:4	Average %	94
	Range %	85-97
1:8	Average %	92
	Range %	84-95

Recovery

The recovery of rat ARG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	95-109
EDTA plasma (n=4)	101	91-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.446	2.345	2.396	2.225
10	1.992	1.914	1.953	1.782
5	1.255	1.326	1.291	1.120
2.5	0.716	0.732	0.724	0.553
1.25	0.420	0.433	0.427	0.256
0.625	0.321	0.338	0.330	0.159
0.312	0.239	0.247	0.243	0.072
0	0.169	0.173	0.171

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Target Background

Function (From Uniprot)	Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.; Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion. In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival. Plays a role in the immune response of alternatively activated or M2 macrophages in processes such as wound healing and tissue regeneration, immune defense against multicellular pathogens and parasites, and immune suppression and allergic inflammation; the regulatory outcome seems to be organ specific. In tumor-infiltrating dendritic cells (DCs) and myeloid-derived suppressor cells (MDSCs) plays a role in suppression of T cell-mediated antitumor immunity.
Gene References into Functions	Arg1 expression, which is abundant in polarized M2 cells, is associated with strain/genotype differences from different pathways. PMID: 25872571 Arginase promotes endothelial dysfunction and hypertension in obesity by reducing arginine bioavailability. PMID: 25557182 Lower activity and expression levels of iNOS and higher activity and expression levels of arginase-1 in rat alveolar macrophages were found to be linked to the susceptibility of T. gondii infection in these cells PMID: 23691079 Diabetes-induced increases in arginase I were involved in the diabetes-induced impairment of retinal blood flow by a mechanism involving vascular endothelial cell dysfunction. PMID: 23232640 Report utility of arginase-1 for monitoring drug-induced liver injury in rat model. PMID: 22872058 Arginase and methylated arginine derivatives appear to be essential modulators of the inflammatory response in the acute phase of a multiple sclerosis model. PMID: 22507752 This study suggested that the increased level of arginase-1 in SCI is associated with an increase in macrophages and reactive astrocytes, possibly contributing to the modulation of inflammation during the course of SCI. PMID: 22325098 we postulate that the increased level of arginase-1, which is partly from M2 macrophages, contributes to the modulation of neuroinflammation in experimental autoimmune encephalomyelitis lesions PMID: 22483960 Arginase contributes significantly to L-proline supply for collagen synthesis in rat fibroblasts, in which arginase I is the predominant isoenzyme PMID: 20107769 These findings reveal the basis for thrombin induction of endothelial arginase I and indicate that arginase inhibition may be an attractive therapeutic alternative in the setting of arterial thrombosis and its associated endothelial dysfunction. PMID: 20032511 crystal structure: arginase-boronic acid complex highlights a physiological role in erectile function PMID: 10542097 Regulates low-level NO production by neuronal NOS (nNOS), most likely by competing for L-arginine. PMID: 11829529 Mechanisms involved in substrate binding and catalysis are elucidated via synthesis and evaluation of alternative substrate. PMID: 12020133 The upregulation of the arginase expression in wound derived fibroblasts underlines the distinct regulation of l-arginine metabolism in WFBs. PMID: 12069499 Total arginase activity and arginase I and II protein expression did not differ between the young and aged groups in the prefrontal cortex. PMID: 15013576 The highest affinity inhibitorsof Arg1 displace the metal-bridging hydroxide ion (and sometimes occupy a Mn(2+)(A) site found vacant in the native enzyme) and maintain a conserved array of hydrogen bonds with their alpha-amino and -carboxylate groups. PMID: 15248756 Arg I plays a critical role in the pathobiology of age-related endothelial dysfunction. PMID: 16380531 Arginase I protein was undetectable in the non-pregnant myometrium and up-regulated at term gestation, contributing to enhanced overall arginase activity at term gestation. PMID: 16735458 Arginase acts as central regulator of trophic factor-deprived motor neuron survival by suppressing nitric oxide production and consequent peroxynitrite toxicity. Resistance of motor neurons to trophic factor deprivation may result from increased arginase. PMID: 16914676 Thus, in addition to enhancing the expression of Arg I and spermine in repaired spinal cords, our treatment may recruit activated macrophages and create a more favorable environment for axonal regrowth. PMID: 17418108 diabetes-induced impairment of vasorelaxation to acetylcholine was correlated with increases in reactive oxygen species and arginase activity and arginase I expression in aorta and liver. PMID: 17967788 Arginase promotes neointima formation in rat injured carotid arteries. PMID: 19164802 upregulation of Arg I and increased synthesis of polyamines play an important role in the conditioning lesion effect, and spermidine is sufficient to promote optic nerve regeneration in vivo PMID: 19641117 Data indicate that iNOS-dependent S-nitrosylation of arginase 1 and the increase in arginase activity lead to eNOS uncoupling, contributing to the nitroso-redox imbalance, endothelial dysfunction, and vascular stiffness observed in vascular aging. PMID: 19661445 Limits iNOS-mediated NO synthese in macrophages, probably by limiting L-arginine availability for iNOS (substrate competition) PMID: 9179379 Show More Hide All
Subcellular Location	Cytoplasm. Cytoplasmic granule.
Protein Families	Arginase family
Tissue Specificity	Detected in liver (at protein level).
Database Links	KEGG: rno:29221 STRING: 10116.ENSRNOP00000017911 UniGene: Rn.9857

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Check Code：

Code	CSB-E17519r
Size	96T,5×96T,10×96T
Trial Size
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.