Rat Glypican-3(GPC3) ELISA kit

Instructions
Code CSB-EL009705RA
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name glypican 3
Alternative Names Gpc3 ELISA Kit; Glypican-3 ELISA Kit; Intestinal protein OCI-5) [Cleaved into: Glypican-3 alpha subunit; Glypican-3 beta subunit] ELISA Kit
Abbreviation GPC3
Uniprot No. P13265
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat GPC3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %95
Range %85-99
1:2Average %107
Range %97-110
1:4Average %89
Range %83-93
1:8Average %99
Range %91-104
Recovery
The recovery of rat GPC3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9484-99
EDTA plasma (n=4)10094-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.506 2.415 2.461 2.280
5002.091 1.974 2.033 1.852
2501.353 1.332 1.343 1.162
1250.813 0.798 0.806 0.625
62.50.484 0.456 0.470 0.289
31.20.408 0.392 0.400 0.219
15.60.274 0.267 0.271 0.090
00.183 0.179 0.181
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Inhibits the dipeptidyl peptidase activity of DPP4 (By similarity). Cell surface proteoglycan that bears heparan sulfate. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition.
Gene References into Functions
  1. Abnormal oncofetal antigen GPC-3 and GPC-3 mRNA expression in hepatocarcinogenesis may be promising molecular markers for early diagnosis of hepatocellular carcinoma. PMID: 21669573
  2. Gpc3 is a marker of hepatic progenitor cells and of early liver lesions. PMID: 17117158
  3. GPC3 may be a negative regulator of liver regeneration and hepatocyte proliferation, and that this regulation may involve CD81. PMID: 19574424
Subcellular Location Cell membrane, Lipid-anchor, GPI-anchor, Extracellular side, Secreted
Protein Families Glypican family
Tissue Specificity Fetal tissues and adult lung.
Database Links

KEGG: rno:25236

STRING: 10116.ENSRNOP00000043344

UniGene: Rn.9717

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