Rat heme oxygenase 1,HO-1 ELISA Kit

Product Details

Citations

Target Data

Most popular with customers

Your Good Partner in Biology Research

Tel : 301-363-4651

Home
Kit
ELISA Kit
Rat heme oxygenase 1,HO-1 ELISA Kit

Name: Rat heme oxygenase 1,HO-1 ELISA Kit
Brand: CUSABIO
SKU: CSB-E08267r

^Instructions

Code	CSB-E08267r
Size	96T,5×96T,10×96T
See More Details

Product Details
Citations
Related Products
Target Data

Target Name

heme oxygenase (decycling) 1

Alternative Names

HO-1, HSP32, bK286B10, heme oxygenase (decyclizing) 1

Abbreviation

HMOX1

Uniprot No.

P06762

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat HO-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-102
1:2	Average %	100
	Range %	96-107
1:4	Average %	86
	Range %	82-90
1:8	Average %	94
	Range %	90-100

Recovery

The recovery of rat HO-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-99
EDTA plasma (n=4)	96	91-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.323	2.252	2.288	2.185
10	1.776	1.692	1.734	1.631
5	1.182	1.105	1.144	1.041
2.5	0.725	0.743	0.734	0.631
1.25	0.465	0.443	0.454	0.351
0.625	0.275	0.259	0.267	0.164
0.312	0.192	0.207	0.200	0.097
0	0.104	0.102	0.103

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Still Have Questions?

Leave a Message or Start an on-line Chat

Mangiferin exerts neuroprotective activity against lead-induced toxicity and oxidative stress via Nrf2 pathway Li H, et al,Chinese Herbal Medicines,2019
PI3K/Akt pathway-mediated HO-1 induction regulates mitochondrial quality control and attenuates endotoxin-induced acute lung injury Shi J, et al,Laboratory Investigation,2019
Protective effects of betanin against paracetamol and diclofenac induced neurotoxicity and endocrine disruption in rats Motawi TK, et al,Biomarkers,2019
Zinc oxide nanoparticle-induced atherosclerotic alterations in vitro and in vivo Zhen Yan.et al,Int J Nanomedicine.,2017
Enhanced hypoxic tolerance by Seabuckthorn is due to upregulation of HIF-1伪 and attenuation of ER stress Kanika Jain.et al,Applied Biomedicine,2016
Pharmacological induction of hemeoxygenase-1 activity attenuates intracerebroventricular streptozotocin induced neurocognitive deficit and oxidative stress in rats Bhardwaj M. et al,Eur J Pharmacol,2016
Neuroprotective effect of hemeoxygenase-1/glycogen synthase kinase-3B modulators in 3-nitropropionic acid-induced neurotoxicity in rats Khan A.et al,Neuroscience.,2015
Pharmacological activation of heme oxygenase (HO)-1/carbon monoxide pathway prevents the development of peripheral neuropathic pain in Wistar rats Bijjem KR et al,Naunyn Schmiedebergs Arch Pharmacol,2013

Function	Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.
Subcellular Location	Microsome, Endoplasmic reticulum membrane, Peripheral membrane protein, Cytoplasmic side
Protein Families	Heme oxygenase family
Database Links	KEGG: rno:24451 STRING: 10116.ENSRNOP00000019192 UniGene: Rn.3160

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mouse 1,25-dihydroxyvitamin D3 (DVD/DHVD3) ELISA kit

Detect Range: 1000 fmol/L-5000 fmol/L

Sensitivity: 250 fmol/L
Human 25-hydroxy vitamin D3,25HVD3 ELISA Kit

Detect Range: 20 μg/L-100 μg/L

Sensitivity: 5 μg/L
Human Arachidonic Acid,AA ELISA Kit

Detect Range: 78.125 ng/mL-5000 ng/mL

Sensitivity: 69.748 ng/mL
Mouse Arachidonic Acid(AA) ELISA Kit

Detect Range: 15.625 ng/mL-1000 ng/mL

Sensitivity: 13.797 ng/mL
Rat Arachidonic Acid(AA) ELISA Kit

Detect Range: 3.125 ng/mL-200 ng/mL

Sensitivity: 2.758 ng/mL
Human apolipoprotein E (Apo-E) ELISA Kit

Detect Range: 15.62 ng/mL-1000 ng/mL

Sensitivity: 3.9 ng/mL
Human adiponectin,ADP ELISA Kit

Detect Range: 1.562 ng/mL-100 ng/mL

Sensitivity: 1.102 ng/mL
Mouse adiponectin,ADP ELISA Kit

Detect Range: 0.156 ng/mL-10 ng/mL

Sensitivity: 0.039 ng/mL