Rat heme oxygenase 1,HO-1 ELISA Kit

Rat heme oxygenase 1,HO-1 ELISA Kit

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Hmox1 Proteins

Hmox1 Proteins

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ELISA Kit
Rat heme oxygenase 1,HO-1 ELISA Kit

Specification
Target

Code

CSB-E08267r

See More Details

Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

P06762

Lead Time

3-5 working days

Abbreviation

HMOX1

Target Name

heme oxygenase (decycling) 1

Alias

HO-1, HSP32, bK286B10, heme oxygenase (decyclizing) 1

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Sandwich

Target Details

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.

HGNC

5013

RGD

2806

MGI

96163

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat HO-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-102
1:2	Average %	100
	Range %	96-107
1:4	Average %	86
	Range %	82-90
1:8	Average %	94
	Range %	90-100

Recovery

The recovery of rat HO-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-99
EDTA plasma (n=4)	96	91-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.323	2.252	2.288	2.185
10	1.776	1.692	1.734	1.631
5	1.182	1.105	1.144	1.041
2.5	0.725	0.743	0.734	0.631
1.25	0.465	0.443	0.454	0.351
0.625	0.275	0.259	0.267	0.164
0.312	0.192	0.207	0.200	0.097
0	0.104	0.102	0.103

References

Pharmacological activation of heme oxygenase (HO)-1/carbon monoxide pathway prevents the development of peripheral neuropathic pain in Wistar rats Bijjem KR et al,Naunyn Schmiedebergs Arch Pharmacol,2013 Jan;386(1):79-90
Neuroprotective effect of hemeoxygenase-1/glycogen synthase kinase-3B modulators in 3-nitropropionic acid-induced neurotoxicity in rats Khan A.et al,Neuroscience.,287:66-77
Pharmacological induction of hemeoxygenase-1 activity attenuates intracerebroventricular streptozotocin induced neurocognitive deficit and oxidative stress in rats Bhardwaj M. et al,Eur J Pharmacol,2016 772: 43-50
Enhanced hypoxic tolerance by Seabuckthorn is due to upregulation of HIF-1伪 and attenuation of ER stress Kanika Jain.et al,Applied Biomedicine,2016
Zinc oxide nanoparticle-induced atherosclerotic alterations in vitro and in vivo Zhen Yan.et al,Int J Nanomedicine.,Jun 13

Still Have Questions?

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Function	Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.
Subcellular Location	Microsome, Endoplasmic reticulum membrane, Peripheral membrane protein, Cytoplasmic side
Protein Families	Heme oxygenase family
Database Links	KEGG: rno:24451 STRING: 10116.ENSRNOP00000019192 UniGene: Rn.3160
Pathway	HIF-1 signaling pathway

Hmox1 Protein

Hmox1 Proteins for Rattus norvegicus (Rat)

Code	Product Name	Source
CSB-YP010583RA	Recombinant Rat Heme oxygenase 1(Hmox1)	Yeast
CSB-EP010583RA CSB-BP010583RA CSB-MP010583RA	Recombinant Rat Heme oxygenase 1(Hmox1)	E.coli Baculovirus Mammalian cell

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Check Code：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.