Rat heme oxygenase 1,HO-1 ELISA Kit

Instructions
Code CSB-E08267r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name heme oxygenase (decycling) 1
Alternative Names Hmox1Heme oxygenase 1 ELISA Kit; HO-1 ELISA Kit; EC 1.14.14.18 ELISA Kit; HSP32 ELISA Kit
Abbreviation HMOX1
Uniprot No. P06762
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat HO-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %98
Range %94-102
1:2Average %100
Range %96-107
1:4Average %86
Range %82-90
1:8Average %94
Range %90-100
Recovery
The recovery of rat HO-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9187-99
EDTA plasma (n=4)9691-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1OD2AverageCorrected
202.323 2.252 2.288 2.185
101.776 1.692 1.734 1.631
51.182 1.105 1.144 1.041
2.50.725 0.743 0.734 0.631
1.250.465 0.443 0.454 0.351
0.6250.275 0.259 0.267 0.164
0.3120.192 0.207 0.200 0.097
00.104 0.102 0.103
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Target Data

Function Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.
Gene References into Functions
  1. HO-1 activation could inhibit cecal ligation and puncture (CLP)-induced lung endoplasmic reticulum stress and attenuate CLP-induced acute lung injury PMID: 30013453
  2. the present study revealed that Bone marrow mesenchymal stem cells protected against liver Ischemia/reperfusion (I/R) injury injury via the promotion of HO1mediated autophagy. PMID: 29956785
  3. The present study indicates that overexpression of HO-1 in bone marrow-derived mesenchymal stem cells enhances the survival and the renal-epithelial differentiation of bone marrow-derived mesenchymal stem cells in the acute kidney injury microenvironment. PMID: 29749549
  4. These finding highlights NOS-dependent modulatory roles for HO. PMID: 29635898
  5. Results imply that octreotide-induced autophagy is dependent on heme oxygenase 1 (HO-1) during hepatic ischemia-reperfusion (I/R). PMID: 29385400
  6. voluntary physical exercise mitigates impaired spatial memory and neuropathological changes exhibited by the aging sedentary group, via elevated HO-1 activity, contributing to the antioxidant capacity in the aging brain. PMID: 29662895
  7. HO-1/BVD/CO pathway activation provides anti-nociceptive and anti-inflammatory effects on the zymosan-induced temporomandibular joint disorders hypernociception in rats PMID: 29362850
  8. n-propargyl caffeamide (PACA) attenuated LPS-induced NF-kB activation while activated Nrf2/HO-1 pathway. HO-1 inhibitor SnPP attenuated the effects of PACA on iNOS expression in LPS-challenged macrophages, possibly by regulating the cross-talk between HO-1 and NF-kB pathways. PMID: 29996121
  9. Effective inhibition of HO-1 expression may contribute to enhancement in the therapeutic effect of Photodynamic therapy (PDT) using talaporfin sodium against malignant meningioma. PMID: 29743446
  10. myricetin treatment significantly increased the activity of Nrf2/HO-1 pathway, strengthening antioxidative stress capacity evidenced by reversed activities of GPx and SOD, and decreased MDA production. PMID: 29147465
  11. findings suggested that L-arginine/NO reduces renal dysfunction associated with I/R of the kidney and may act as a trigger to regulate the NF-kappaB, HSP70 and Nrf2/HO-1 signaling cascades. PMID: 28854440
  12. autophagy is involved in the protective effects of HO-1/BM-MSCs on the transplanted liver after RSLT. Regulation of autophagy is primarily mediated through the ERK/mTOR signaling pathway. PMID: 28901391
  13. This study shows that systemic CO treatment can promote an anxiolytic-like effect and the expression of HO-1 enzymes in locus coeruleus cells. PMID: 28887196
  14. Abeta(1-42) is toxic to astrocytes, but induction of HO-1 affords protection against this toxicity due to formation of CO PMID: 28617444
  15. heme oxygenase-1-in bone marrow mesenchymal stem cells has an effect on microcirculation and energy metabolism following liver transplantation PMID: 28596681
  16. results implied that HO-1 upregulation suppressed oxidative stress induced by LPS, and the possible mechanism could be associated with Mfn1 and the PI3K/Akt pathway. PMID: 27830717
  17. Cardioprotective Effect of Selective Estrogen Receptor Modulator Raloxifene Are Mediated by Heme Oxygenase in Estrogen-Deficient Rat PMID: 28770019
  18. This study points to the modulatory effect of C. molmol on glutamate-NO-cGMP and Nrf2/ARE/HO-1 pathways in hyperammonemia. Therefore, C. molmol might be a promising protective agent against hyperammonemia. PMID: 28744340
  19. SERPINB1 decreased inflammation, ameliorated oxidative stress in the lung, and attenuated acute lung injury in rats with orthotopic autologous liver transplantation by activating HO-1 and it does so through STAT3 PMID: 28427999
  20. NIC pretreatment effectively inhibit CME-induced myocardial apoptosis and improve cardiac function. The protective effects are mediated through the activation of the Nrf2/HO-1 signaling in cardiomyocytes. PMID: 29412163
  21. H2O2 induced expression of HO-1. Exogenous HO-1 reduced phosphorylation of MLK3, MKK7 and JNK3. Consistent with its inhibitory effect on MLK3/MKK7/JNK3 signaling, exogenous HO-1 decreased H2O2-induced neuronal apoptosis and necrosis. It inhibited expression of Cdc42. Down-regulation of MLK3/MKK7/JNK3 signaling might be related to up-regulation of microRNA-137. PMID: 27864650
  22. Ischemic preconditioning attenuates ischemia/reperfusion injury in rat steatotic liver via heme oxygenase-1-mediated autophagy. PMID: 27852058
  23. Together with results showing involvement of TTF-1 in the TNF-alpha-induced increase in interleukin 1 beta and monocyte chemotactic protein 1 production, this study suggests that TTF-1 plays an important role in the mouse hypothalamus TNF-alpha-induced inflammatory response for regulating HO-1 gene expression. PMID: 29305861
  24. The renoprotection by hemin was accompanied by the reduction of inflammatory/oxidative mediators including, macrophage-inflammatory-protein-1alpha, macrophage-chemoattractant-protein-1 and 8-isoprostane, whereas HO-1, HO-activity and the total-anti-oxidant-capacity increased. PMID: 25460740
  25. Alpha-melanocyte-stimulating hormone induces vasodilation and exerts cardioprotection through the heme-oxygenase pathway in rat hearts. PMID: 28195947
  26. Since the rat is the preferred laboratory animal in experimental physiology and pathophysiology, the rat model of HO-1 deficiency may provide a novel tool for investigation of the role of this enzyme in renal function and disease. PMID: 27778153
  27. Downregulation of histone acetylation can inhibit manganese chloride-induced Nrf2 nuclear translocation and further inhibits the Nrf2/HO-1 pathway in manganese neurotoxicity syndromes. PMID: 29054681
  28. Fructose decreases HO-1 expression in maternal liver and placenta. PMID: 27545118
  29. Hemoglobin-activated astrocytes acquired resistance to hemin-induced toxicity via Nrf2/HO-1 pathway. This phenomenon can be considered as the adaptive self-defense in the pathological process of intracerebral hemorrhage. PMID: 29074371
  30. Biliary tract external drainage BTED protected multiple organs against severe acute pancreatitis related injuries via HO-1 upregulation. PMID: 28209258
  31. The expression of Nrf2/HO-1 increased in mesenteric arteries of portal hypertensive rats, which was related to oxidative stress. HO-1was involved in increased portal pressure and anomaly splanchnic hemodynamics in portal hypertensive rats. PMID: 28025064
  32. In order to elucidate the possible role of HO-1 in mediating the protective effects of ghrelin, tin protoporphyrin (SnPP) HO-1 blocker was administrated; it significantly attenuated the gastroprotective effect of ghrelin. In conclusion HO-1 activity significantly contributes toward ghrelin-mediated gastroprotection. PMID: 28635609
  33. HO-1 is a physiologic regulator of glomerular decay accelerating factor (DAF) and identify hemin analogues as inducers of functional glomerular DAF able to minimize complement component C3 fragment b (C3b) deposition. PMID: 27662796
  34. HO-1 Protects against Hypoxia/Reoxygenation-Induced Mitochondrial Dysfunction in H9c2 Cardiomyocytes PMID: 27138700
  35. the results of this study suggest that extratumoral HO-1+ macrophages may have an important role in prostate cancer. PMID: 27280718
  36. this study shows that overexpression of HO-1 in bone marrow mesenchymal stem cells increased their immunomodulatory effects protected liver allografts PMID: 28069109
  37. this experiments shown here indicate that HO-2 and HO-1 are differentially regulated across sexual experience and that the two CO-producing enzymes are expressed in distinct but overlapping populations of cells in medial preoptic area. PMID: 28088559
  38. Data show that nonylphenol (NP) increased serum alkaline phosphatase, lead to liver damage and can increase the expression of heme oxygenase-1 (HO1) and growth arrest and DNA-damage-inducible 45 beta protein (Gadd45b) genes and may modify the toxic effects on liver through induction of oxidative stress. PMID: 27590577
  39. Heme oxygenase 1 induction protects against diabetic nephropathy in streptozotocin-induced diabetic rats. PMID: 28229631
  40. remote ischemic preconditioning protects hepatic microcirculation by induction of HO and modulation of cytokine induced neutrophil chemoattractant in hepatic ischemia reperfusion injury PMID: 27672274
  41. Heme oxgenase-1 expression in concanavalin A-stimulated T cells that were co-cultured with adipose-derived stem cells revealed a significant increase compared with concanavalin A-stimulated T cells alone. TGF-beta and interleukin-10 increased and interferon-gamma decreased in the adipose-derived stem-cell-co-cultured T-cell group compared with other groups. A heme oxgenase inhibitor protected against these changes. PMID: 27391839
  42. HO-1 expression in response to Cd maintains cellular homeostasis during oxidative stress in NRK52E kidney cells PMID: 27658547
  43. alpha-Boswellic acid decreases oxidative stress. Nrf2/HO-1 pathway might play a role in the gastroprotective action of alpha-BA in ethanol-induced gastric injury in rats. PMID: 26992040
  44. The induced expression of HO-1 by fenofibrate appeared to be essential for mediating the protective effects of fenofibrate, as the inhibition of HO-1 activity significantly diminished the protective effects of fenofibrate against the GM-mediated death of sensory hair cells in cochlea PMID: 28290603
  45. Ethylbenzene induced oxidative stress, apoptosis, and up-regulation of HO-1 and Nrf2 in NRK-52E cells. PMID: 26381691
  46. HO-1 activity and protein are increased significantly in rats with acute liver injury induced by LPS, suggesting that HO-1 plays an important role in the pathogenesis of acute hepatic damage. PMID: 27432233
  47. 18beta-GA has a protective effect on MTX-induced nephrotoxicity with possible mechanisms of attenuating oxidative stress and inflammation through up-regulating the Nrf2/ARE signaling PMID: 27499091
  48. Intermittent hypoxia exposure also increased hepatic expression of HO-1 and iron-binding protein ferritin-1 at the late phase, in association with increase in serum iron, bilirubin, and hepatic levels of lipid peroxides, such as 4-hydroxy-2-nonenal (HNE). PMID: 27021659
  49. Expression of tumor necrosis factor (TNF)alphainduced protein 8-like 2 (TIPE2), matrix metalloproteinase-9 (MMP-9) and heme oxygenase-1 (HO-1) and mitogen-activated protein kinase (MAPK) signaling was modulated by Xuebijing injection (XBJ). PMID: 27666960
  50. rats treated with PAN. CONCLUSION: Depending on the type of injury, HO-1 overexpression in GEC may or may not reduce proteinuria. Reduced macrophage infiltration and preservation of nephrin expression are putative mechanisms underlying the protective effect of HO-1 overexpression following immune injury PMID: 27442135
  51. These results suggest that hawthorn extract could have protective effects in ovariectomized rats by improving lipid profiles, decreasing oxidative stress, and improving the antioxidant defense system. PMID: 27187458
  52. Heme oxygenase-1 alleviates vascular complications associated in metabolic syndrome through maintaining endothelial-dependent relaxation and NO generation in addition to improving insulin sensitivity. PMID: 25268984
  53. Triptolide reduces I/R-induced myocardial infarction, inflammation, oxidative stress and improves the cardiac function in rats. Cardioprotective effects of triptolide might be associated with its effect on Nrf2/HO-1 defense pathway. PMID: 26391895
  54. HO-1 inhibited expression of activating transcription factor 4 (ATF4), which is a transcription factor regulating NLRP1 expression PMID: 26925775
  55. Simvastatin may protect the kidney from oleic acid induced oxidative stress by upregulating the antioxidant HO-1 via CREB. PMID: 26492285
  56. This study suggests that decursin extracted from A. gigas is a supplemental agent that can reduce oxidative stress by Nrf2-mediated upregulation of HO-1 in rat experimentally induced unilateral cryptorchidism and may improve cryptorchidism-induced infertility. PMID: 27034737
  57. Sulforaphane (SFN) increased the expression of Nrf2 and HO-1 in CIN rats and in Ioversol-induced injury HK2 cells. SFN increased cell viability and attenuated ROS level in vitro. Conclusions. SFN attenuates experimental CIN in vitro and in vivo. This effect is suggested to activate the Nrf2 antioxidant defenses pathway. PMID: 27006750
  58. this study provides some evidence that impairment of NO signaling pathway in intrapulmonary arteries involves HO-1 PMID: 26780163
  59. HO-1 promotes wound closure by augmenting anti-inflammation, antioxidant, and angiogenesis pathways in diabetic rats. PMID: 26798657
  60. Hemin pretreatment, by enhancing HO-1 induction, increased lung antioxidant capacity and reduced inflammatory stress. PMID: 26838179
  61. increased resistance to hypoxia and improved therapeutic potential of EPCs as a result of hypoxia preconditioning is mediated via the PI3K/Akt-Nrf2-HO-1 signaling pathway, and the secretion of HIF-1 alpha followed by Nrf2 activation PMID: 26709780
  62. Inflammation and edema of the lung and kidney of hemorrhagic shock rats are alleviated by biliary tract drainage via the HO-1 pathway. PMID: 26253294
  63. In MCA occlusion, KBA increased the protein HO-1 expression in ischemic brain tissues and primary cultured astrocytes, providing protection against oxygen/glucose deprivation induced oxidative insult. HO-1 knockdown attenuated the KBA protective effect. PMID: 25452227
  64. EGCG down-regulated NLRP3 expression, which was blocked by ZnPP, indicating that HO-1 links EGCG with NLRP3 PMID: 26866373
  65. Heme oxygenase-1 expression in the brain after heatstroke PMID: 26392811
  66. induction of oxygenase-1 with cobalt protoporphyrin reduces the degree of microalbuminuria and has renal protective effects and regulating the glomerular VEGF-NO axis in diet-induced obese rats by increasing adiponectin levels PMID: 25959017
  67. N-acetylcysteine upregulated HO-1 through the activation of Nrf2 pathway and protected rat against CCl4-induced liver injury. PMID: 26339453
  68. the results provide evidence that EPS exerts new beneficial effects on endothelial cells by increasing GSH, thioredoxin, and heme oxygenase-1 levels through the activation of Nrf2. PMID: 25529839
  69. Carbon monoxide-releasing molecule-2-enhanced HO-1 promoter activity was inhibited by co-transfection with small interfering RNA (siRNA) of c-Jun, specificity protein 1 (Sp1), or nuclear factor-erythroid 2-related factor 2 (Nrf2). PMID: 25148934
  70. The proximal tubules demonstrate a corresponding up-regulation of the cytoprotective protein heme oxygenase-1 (HO-1) which is capable of mitigating acute kidney injury. PMID: 25904107
  71. Data show that induction of heme oxygenase-1 (HO-1) promoted a protective M2-like milieu in the pancreas and recruited mesenchymal cells, M2 macrophages, and fibrocytes that imparted immunoregulatory and pro-repair effects, preventing type 1 diabetes. PMID: 26252059
  72. Heme oxygenase-1/signal transducer and activator of transcription 3 signaling plays a critical role in propofol-mediated amelioration of hyperglycemia-induced cardiomyocyte hypertrophy and apoptosis. PMID: 24810525
  73. Data show that heme oxygenase-1 (HMOX1)nduction by heme increases hepatocyte transporter expression, subsequently stimulating bile flow in cholestasis. PMID: 25683492
  74. Eevation of HO-1 expression reduced the OALT-induced intestinal mucosa injury and TJ dysfunction. PMID: 26064429
  75. Pitavastatin can enhance endogenous HO-1 expression in mesenchymal stem cells, which may protect the cells into the environment of oxidative stress with partial activation of endothelial nitric oxide synthase and Akt phosphorylation. PMID: 25714596
  76. Heme oxygenase improves renal function by potentiating podocyte-associated proteins in l-NAME-induced hypertension. PMID: 25498996
  77. Suggest that totarol may be a novel activator of the Akt/HO-1 pathway protecting against ischemic stroke through reduction of oxidative stress. PMID: 26440581
  78. It was concluded that induction of HO-1 and HSP70 by celastrol prevents multiorgan injury and circulatory failure in endotoxemia. PMID: 25571843
  79. HO-1 is an anti-inflammatory factor in lipopolysaccharide-stimulated ovary, which regulates the inflammatory response through downregulation of p38 signaling pathways in rat ovary. PMID: 25448262
  80. It was concluded that experimentally induced features of neurodegenerative disorders in hippocampal cultures was offset by alpha7 nAChR activation via heme-oxygenase 1. PMID: 26212551
  81. decreases LPS-induced liver inflammation by down-regulation of p38 signaling pathway PMID: 26286380
  82. the present findings suggested that curcumin may be significant in cellular antioxidant defense, through the activation of Nrf2 and HO-1, thereby protecting the NRK-52E cells from HG-induced EMT. PMID: 25823828
  83. vitamin E supplementation-via induction of the cytoprotective HO-1-may help to reduce renal oxidative stress imposed by smoking or obesity PMID: 25471815
  84. HO-1 induction with CoPP improves FFA-induced endothelial dysfunction in the rat aorta. The protective mechanism appears to be related to the activation of the AMPK-PI3K-eNOS pathway PMID: 25766533
  85. Vitamin A deficiency reduced serum iron and transferrin saturation levels, increased spleen iron concentrations, reduced hepatic Hamp and kidney erythropoietin messenger RNA levels and up-regulated hepatic and spleen heme oxygenase-1 gene expression. PMID: 24998947
  86. HO-1 protects liver against I/R injury by inhibiting TLR2/TLR4-triggered MyD88- and TRIF-dependent signaling pathways and increasing expression of negative regulators of TLR signaling in rats. PMID: 25780291
  87. HMOX induction affects the GM1 ganglioside distribution in the cholestatic hepatocyte. PMID: 24564601
  88. Intestinal remote ischemic conditioning attenuates hepatic ischemia/reperfusion injury, presumably by HO-1 induction in hepatocytes. PMID: 25234134
  89. indicate presence of a HO-1 expression control mechanism in the glomerulus that may serve to protect it against potentially detrimental effects of exaggerated HO-1 expression PMID: 25824035
  90. lithium protects against ventricular arrhythmias by attenuating NGF-induced sympathetic innervation via antioxidant activation of the Nrf2/HO-1 axis. PMID: 25224036
  91. The present data clearly demonstrate that THC protects LPS-challenged septic kidney by decreasing macrophage infiltration and increasing HO-1 expression PMID: 25091807
  92. these results provide the mechanism whereby the benefits of HO-1 target in TLR-4 signaling pathway. HO-1 could be then an interesting target to protect islets before transplantation PMID: 25343247
  93. Celastrol induces Hsp32 expression in glial cells in cerebral cortical cultures. PMID: 24700193
  94. Delayed remote ischemic preconditioning may produce an additive cardioprotection to sevoflurane postconditioning through an enhanced HO-1 expression partly via Nrf2 translocation. PMID: 24651515
  95. compared to age-matched controls, we observed in HIV-1 transgenic rats a significant reduction in the protein levels of Nrf2 and HO-1, suggesting a weakening in the protection exerted by Nrf2/HO-1 system PMID: 25027760
  96. The upregulation of HO1 was found to suppress cilostazolinduced cellular senescence. PMID: 25175370
  97. results suggest that IL-10/HMOX1 signaling is involved in modulation of cochlear inflammation through inhibition of MCP-1/CCL2 regulation in SLFs PMID: 25780042
  98. Results show that HO-1 induces mitochondrial dysfunc-tion, and cellular pathology under certain growth conditions. PMID: 24494190
  99. NMR relaxation experiments showed that the heme-binding site in heme-free HO-1 fluctuates in concert with a surface-exposed loop and transiently forms a partially unfolded structure. PMID: 25496210
  100. Results imply that HO-1 can decrease cell apoptosis in the ischemia/reperfusion rat brain and that the mechanism may be related to the activation of the BDNF-TrkB-PI3K/Akt signaling pathway PMID: 24997248

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Subcellular Location Microsome, Endoplasmic reticulum membrane, Peripheral membrane protein, Cytoplasmic side
Protein Families Heme oxygenase family
Database Links

KEGG: rno:24451

STRING: 10116.ENSRNOP00000019192

UniGene: Rn.3160

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