Human follicle-stimulating hormone,FSH ELISA Kit

Code CSB-E06867h
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details


The human FSH ELISA kit is a solid-phase immunoassay specially designed to quantitatively measure human FSH in the samples. It is based on the Sandwich-ELISA mechanism. FSH in the sample is bound to the capture antibody immobilized on the 96-well strip plate and then sandwiched with the biotinylated FSH antibody. After the addition of HRP-avidin and TMB substrate, the solution in the wells turns blue. The color reaction is stopped by adding the stop solution into the wells, and the color changes from blue to yellow. The color intensity is positively proportional to the FSH bound in the initial step. The FSH concentration can be calculated according to the standard curve.

FSH is a hormone produced by the anterior pituitary in response to the gonadotropin-releasing hormone (GnRH) from the hypothalamus. It plays a crucial role in the maintenance of normal functioning of the reproductive system in men and women. FSH aids in the regulation of the menstrual cycle in women and increases the production of eggs in the ovaries. It causes granulosa cells in ovarian follicles to create aromatase, an enzyme that converts androgens produced by the thecal cells to estrogen. FSH is required for men to maintain normal sperm count and function. Unresponsive gonads or hyperfunctioning pituitary adenomas are linked to elevated FSH levels. Low FSH levels have been linked to hypothalamic or anterior pituitary dysfunction.

Alternative Names FSHBFollitropin subunit beta ELISA kit; Follicle-stimulating hormone beta subunit ELISA kit; FSH-B ELISA kit; FSH-beta ELISA kit; Follitropin beta chain ELISA kit
Abbreviation FSH
Uniprot No. P01225
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 2 mIU/ml-50 mIU/ml.
Sensitivity 1 mIU/ml.
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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I am curious about the specificity of this kit. Are the antibodies only specific to FSH Beta?

Thanks for your inquiry. Yes the antibodies are only specific to FSH Beta. Beta is the specific subunit of FSH.

Target Background

(From Uniprot)
Together with the alpha chain CGA constitutes follitropin, the follicle-stimulating hormone, and provides its biological specificity to the hormone heterodimer. Binds FSHR, a G protein-coupled receptor, on target cells to activate downstream signaling pathways. Follitropin is involved in follicle development and spermatogenesis in reproductive organs.
Gene References into Functions
  1. High follicle stimulating hormone expression is associated with Turner syndrome. PMID: 29415703
  2. We found a genetic association between rs11031006 (FSHB) SNP and endometriosis. WNT4 and VEZT genes constitute the most consistently associated genes with endometriosis. In the present study, an association of rs7521902 (WNT4) and rs10859871 (VEZT) was confirmed in women with endometriosis at the genotypic but not the allelic level. PMID: 28901453
  3. the in vivo bioactivities of FSH glycoforms in Fshb null mice using a pharmacological rescue approach, was investigated. PMID: 27561202
  4. Genetic variation affecting FSH production (FSHB c.-211G>T) was associated with age at pubertal onset, as assessed by testicular enlargement. The effect appeared further modified by coexistence of genetic variation affecting FSH sensitivity (FSHR c.-29G>A). PMID: 28323923
  5. A novel homozygous mutation in the FSHbeta gene (c.343C > T) identified in a male patient with isolated FSH deficiency and infertility. PMID: 28392474
  6. Association of the FSHB 211G > T polymorphism with male infertility in Han-Chinese PMID: 28764642
  7. Genetic variations of FSH signaling appear to determine the individual set point of the hypothalamic-pituitary-gonadal axis already early in life. PMID: 27270476
  8. Modeling and high-throughput experimental data uncover the mechanisms underlying Fshb gene sensitivity to gonadotropin-releasing hormone pulse frequency PMID: 28385888
  9. Variants in FSHB gene are associated with polycystic ovary syndrome and LH levels in Han Chinese women. PMID: 26938199
  10. Data indicate that compared with normal eggs, oocytes with a brown zona pellucida (ZP) were found to have a higher ZP thickness (ZPT), lower embryo quality and lower pregnancy rate, which might be due to a high gonadotropin (Gn) dosage injection and high serum follicle stimulating hormone (FSH) levels. PMID: 27442586
  11. genetic association studies in peripubertal girls in Denmark: Data suggest that an SNP in FSHR (follicle stimulating hormone receptor, c.2039A>G) is associated with blood levels of FSH, LH, and estradiol; minor alleles in FSHB (follicle stimulating hormone beta subunit, c.-211G>T) and FSHR (c.-29G>A) are associated with delayed pubertal onset. PMID: 26905078
  12. The FSHB -211G>T variant attenuates serum FSH levels in the supraphysiological gonadotropin setting of Klinefelter syndrome PMID: 25052309
  13. 3 known FSH-action modulating SNPs (FSHB -211G/T; FSHR -29G/A, c.2039 A/G)explained together 2.3%, 1.4%, 1.0 and 1.1% of the measured variance in serum FSH, Inhibin B, testosterone and total testes volume, respectively. PMID: 24718625
  14. The combined effect of FSHB GG+FSHR AA may potentiate the FSH pathway, which increases serum levels of FSH and reduces antimullerian hormone in health girls. PMID: 23850305
  15. Serum FSH levels are affected by the combination of genetic polymorphisms in FSHR and FSHB PMID: 23504007
  16. Data suggest that low serum FSH levels in men with an SNP in promoter region of FSHB (-211G/T) result from reduced binding of LHX3 (LIM homeobox 3) to FSHB promoter/response element and down-regulation of FSHB transcription in gonadotrophs. PMID: 23766128
  17. activin-regulated transcription mediated by forkhead box L2 [review] PMID: 23426431
  18. Data indicate that neither serum inhibin B nor follicle-stimulating hormone (FSH) is a suitable surrogate for determination of sperm concentration in a semen sample. PMID: 23423746
  19. Ser680 genotype for FSHR is a factor of relative resistance to FSH stimulation resulting in slightly higher FSH serum levels, thus leading to a prolonged duration of the menstrual cycle. PMID: 23380184
  20. Phenotypic consequences of FSHB -211G-->T on the hypothalamic-pituitary-ovarian axis in women. PMID: 23118424
  21. Both estradiol and progesterone uniquely modulate basal and GnRH-stimulated gonadotropin promoters without affecting cell growth. PMID: 23160221
  22. Negative fetal FSH/LH regulation in late pregnancy is associated with declined kisspeptin/KISS1R expression in the tuberal hypothalamus. PMID: 23015653
  23. In the proposed model of the combined effects, FSHB -211G>T acts strongly on male reproductive parameters, whereas the FSHR 2039A>G effects were approximately 2-3 times smaller. PMID: 22791757
  24. [review] A middle-aged male with feminizing adrenocortical (ACC) tumor shows delayed response of luteinizing hormone and poor response of FSH to gonadotropin-releasing hormone administration. PMID: 21720063
  25. FSHB -211 TT genotype might represent a novel treatable form of male infertility characterized by severe spermatogenic impairment and low or inappropriately normal FSH plasma levels. PMID: 22000911
  26. We showed for the first time that genetically determined low FSH may have wider downstream effects on the male reproductive system, including impaired testes development, altered testicular hormone levels and affected male reproductive potential. PMID: 21733993
  27. Polymorphisms in the 5'-flanking regions of FSHbeta and luteinizing hormone (LH)beta genes are probably related to the puberty onset time of Chinese Han girls. PMID: 20869425
  28. FSH dysfunction due to a less acidic isoform pattern through hypoglycosylation is not a key mechanism of primary ovarian insufficiency in classic galactosemia PMID: 20814826
  29. Smad2 expression in ovarian cells requires a functional interplay between activin A (INHBA) and FSH/PKA signaling. PMID: 12665510
  30. The level of serum FSH may be related to bone loss and the occurrence of osteoporosis in Chinese women. PMID: 19734592
  31. Findings demonstrate that FSH has dose-dependent anabolic effects on bone via an ovary-dependent mechanism. PMID: 21149714
  32. the genomic and genetic context of the FSHB and the LHB/CGB genes largely affects the profile of the hormone production PMID: 20488225
  33. FoxL2 plays a key role in activin induction of the FSHbeta gene, by binding to sites conserved across multiple species. PMID: 20233786
  34. This genetic marker may have clinical significance in molecular diagnostics of male reproductive success. PMID: 19897680
  35. Review. The Asn7 and Asn24 glycosylation variants of human pituitary FSHB exhibit differential and divergent effects at the target cell level and that less sialylated, short-lived variants may exert significant effects in in vivo conditions. PMID: 11900895
  36. FSH beta gene mutations in a female with partial breast development and a male sibling with normal puberty and azoospermia PMID: 12161499
  37. Follicle-stimulating hormone interacts with exoloop 3 of the receptor. PMID: 12374801
  38. A Cys82Arg mutation in this protein causes FSH deficiency in FSH levels in vitro. PMID: 12568849
  39. No mutation in this gene can still cause FSH deficiency in a person with normal virilization. PMID: 12568861
  40. x-ray crystallographic analysis shows that the FSH beta subunit is close to the N-terminal region and the alpha subunit is projected toward exoloop 3 in the endodomain PMID: 12963710
  41. pituitary exit of LH and FSH occur via different secretion pathways, and are released spatially from the pituitary via different circulatory routes. PMID: 14585810
  42. LHX3 LIM homeodomain transcription factor is involved in activation of the FSH beta-subunit gene in the pituitary gonadotrope cell. PMID: 15271874
  43. Sequence analysis shows that the human FSH beta gene is highly conserved and amino acid changing mutations are apparently extremely rare; only three silent polymorphisms, two of which are novel, are revealed. PMID: 16100240
  44. The strategy to produce a soluble FSH-FSHR complex that involves the co-secretion of a truncated FSHR ectodomain (FSHR(HB)) and a covalently linked FSHalphabeta heterodimer, is described. PMID: 17045735
  45. A major regulatory mechanism for FSH glycosylation involves control of beta-subunit N-glycosylation, possibly by inhibition of oligosaccharyl transferase. Two primate species exhibited the same all-or-none pattern of pituitary FSHbeta glycosylation. PMID: 17079072
  46. There is a preliminary observation suggesting that the second most frequent FSHB haplotype may be associated with rapid conception success in females. PMID: 17227474
  47. Follitropin beta and, more precisely, follitropin alfa suppress the spectral components and power of the myoelectrical signals, which provides uterine quiescence. PMID: 18068163
  48. Case Report: Isolated FSH deficiency without mutation of the FSHbeta gene and successful treatment with human menopausal gonadotropin. PMID: 18402948
  49. A polymorphism within the FSHB gene is associated with serum FSH levels in men. PMID: 18567894
  50. Ghrelin suppresses the secretion of FSH in humans. PMID: 19054012

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Involvement in disease Hypogonadotropic hypogonadism 24 without anosmia (HH24)
Subcellular Location Secreted.
Protein Families Glycoprotein hormones subunit beta family
Database Links

HGNC: 3964

OMIM: 136530

KEGG: hsa:2488

STRING: 9606.ENSP00000254122

UniGene: Hs.36975

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