Product Details

Purity

>97% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion in murine NIH/3T3 cells is less than 10 ng/ml, corresponding to a specific activity of >1.0x10⁵ IU/mg.

Target Names

Il36g

Uniprot No.

Q8R460

Research Area

Immunology

Alternative Names

Il36g; Il1f9Interleukin-36 gamma; Interleukin-1 family member 9; IL-1F9

Species

Mus musculus (Mouse)

Source

E.Coli

Expression Region

13-164aa

Complete Sequence

GRETPDFGEV FDLDQQVWIF RNQALVTVPR SHRVTPVSVT ILPCKYPESL EQDKGIAIYL GIQNPDKCLF CKEVNGHPTL LLKEEKILDL YHHPEPMKPF LFYHTRTGGT STFESVAFPG HYIASSKTGN PIFLTSKKGE YYNINFNLDI KS

Mol. Weight

17.3 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free

Form

Liquid or Lyophilized powder

Buffer

Lyophilized from a 0.2 ?m filtered solution in 1 M MOPS, 10 mM NaAC, pH7.6, with 2 mM EDTA, 5 % Trehalose, 0.02 % Tween-20.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Datasheet & COA

Please contact us to get it.

Description

IL-36γ drives pro-inflammatory signaling through NF-κB and MAPK pathways, making precise functional characterization dependent on a highly potent, endotoxin-controlled reagent. This tag-free recombinant mouse IL-36γ, encompassing the full mature sequence (aa 13–164), demonstrates an ED50 of less than 10 ng/mL in IL-6 secretion assays using murine NIH/3T3 cells, corresponding to a specific activity exceeding 1.0 × 10⁵ IU/mg—sufficient potency for cell-based activation assays, NF-κB/MAPK signaling studies, and use as a reference standard in cytokine detection platforms. Endotoxin levels below 1.0 EU/μg ensure that downstream inflammatory readouts reflect genuine IL-36γ activity rather than LPS-driven artifacts, which is critical for in vivo murine inflammation models and primary cell experiments. Purity exceeding 97% by SDS-PAGE, combined with this stringent endotoxin profile, supports use in antibody development workflows including immunization, ELISA coating, and functional blocking assays.

Target Background

Function

Functions as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2/IL1RL2. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. May play a role in proinflammatory responses during particular neutrophilic airway inflammation. May be involved in the innate immune response to fungal pathogens. Induces the production of proinflammatory cytokines in bone marrow-derived dendritic cells (BMDCs), including IL-12, Il-1 beta, IL-6, TNF-alpha and IL-23. Involved in dendritic cell maturation by stimulating the surface expression of CD80, CD86 and MHC class II. Induces the production of IFN-gamma, IL-4 and IL-17 by cultured CD4(+) T-cells and splenocytes.

Subcellular Location

Cytoplasm. Secreted.

Protein Families

IL-1 family

Database Links

UniGene: Mm.249379

Code	CSB-AP003131MO
Abbreviation	Recombinant Mouse Il36g protein (Active)
MSDS	MSDS Datasheet
Size	$354
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Mouse Interleukin-36 gamma protein (Il36g) (Active)

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