Code | CSB-MA848820A0m |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:1000-1:5000 |
The production of the Alix monoclonal antibody begins with immunizing mice with recombinant human PDCD6IP protein. B cells from the spleen of the immunized mice are then fused with myeloma cells to generate hybridoma cells. The hybridoma cells are screened to identify the cell line that produces the Alix antibody. The Alix monoclonal antibody is purified from the mouse ascites using protein A affinity chromatography, resulting in a purity of over 95%. The antibody is an unconjugated IgG2b type and can be used in ELISA and WB applications to specifically recognize the human Alix protein.
Alix, also known as PDCD6IP, mainly functions in endosomal sorting and multivesicular body (MVB) biogenesis. Alix interacts with the endosomal sorting complex required for transport (ESCRT) machinery to mediate the sorting of ubiquitinated proteins into MVBs for degradation. Alix also plays a role in cytokinesis, virus budding, and apoptosis. It is involved in the regulation of intracellular calcium signaling and has been implicated in a variety of diseases, including cancer, HIV infection, and neurodegenerative disorders.
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