Product Details

Uniprot No.

Target Names

Alternative Names

Programmed cell death 6-interacting protein (PDCD6-interacting protein) (ALG-2-interacting protein 1) (ALG-2-interacting protein X) (Hp95), PDCD6IP, AIP1 ALIX KIAA1375

Species Reactivity

Human, Rat

Immunogen

A synthesized peptide derived from human ALIX

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

7E9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
FC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

PDCD6IP, commonly known as ALIX, serves as a critical adaptor protein within the ESCRT pathway, orchestrating membrane remodeling events essential for exosome biogenesis, viral budding, and cytokinetic abscission. Its central role in these fundamental cellular processes makes ALIX a compelling target for researchers investigating extracellular vesicle biology, viral pathogenesis, and membrane trafficking mechanisms.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human ALIX, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and production occurs in a controlled recombinant system, researchers can expect uniform performance across experiments and over time, eliminating the lot-to-lot variability that can complicate long-term studies or multi-site collaborations.

Validation studies demonstrate reliable detection across diverse sample types in western blot applications, with the antibody successfully identifying ALIX at the expected 97 kDa molecular weight in human cell lines including L02, HepG2, PC-3, and U-87, as well as in rat liver tissue. This cross-species reactivity between human and rat samples provides flexibility for researchers working across model systems. Flow cytometry validation in Jurkat cells confirms utility for single-cell analysis, expanding the range of experimental approaches available with this reagent.

The antibody's performance across multiple validated applications, from population-level protein quantification via western blot to single-cell resolution through flow cytometry, supports comprehensive characterization of ALIX expression and function. Whether investigating exosome secretion pathways, examining viral exploitation of host machinery, or exploring ESCRT-mediated signaling events, this antibody provides a dependable tool for advancing understanding of this multifunctional scaffold protein.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Multifunctional protein involved in endocytosis, multivesicular body biogenesis, membrane repair, cytokinesis, apoptosis and maintenance of tight junction integrity. Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. May play a role in the regulation of both apoptosis and cell proliferation. Regulates exosome biogenesis in concert with SDC1/4 and SDCBP. By interacting with F-actin, PARD3 and TJP1 secures the proper assembly and positioning of actomyosin-tight junction complex at the apical sides of adjacent epithelial cells that defines a spatial membrane domain essential for the maintenance of epithelial cell polarity and barrier.; (Microbial infection) Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function requires the interaction with CHMP4B. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as enveloped virus budding (HIV-1 and other lentiviruses).

Gene References into Functions

our results identify the CD63-syntenin-1-ALIX complex as a key regulatory component in post-endocytic HPV trafficking. PMID: 27578500
Alix acts in concert with endophilin A to promote clathrin-independent endocytosis of cholera toxin and to regulate cell migration. PMID: 27244115
Revealed the transition of diffuse ALIX protein signals into a multivesicular body-like pattern during adenoma-carcinoma sequence in colorectal neoplasms. PMID: 27150162
Alix plays an important role in the proliferation of glioma cells and overexpression in gliomas predicts poor survival. PMID: 26980041
ALIX regulates P2Y1 degradation. PMID: 27301021
farnesylation of K-Ras was required for its packaging within extracellular nanovesicles, yet expressing a K-Ras farnesylation mutant did not decrease the number of nanovesicles or the amount of Alix protein released per cell. PMID: 27909058
These findings indicate that Alix binds to Ago2 and miRNAs, suggesting that it plays a key role in miRNA enrichment during extracellular vesicles biogenesis. PMID: 26935291
The authors find that HIV-1 nucleocapsid mimics the PDZ domains of syntenin, a membrane-binding adaptor involved in cell-to-cell contact/communication, to capture the Bro1 domain of ALIX, which is an ESCRTs recruiting cellular adaptor. PMID: 26962944
We found that ARRDC3 is required for ALIX ubiquitination induced by activation of PAR1 PMID: 26490116
phosphorylation of the intramolecular interaction site in the PRD is one of the major mechanisms that activates the ESCRT function of ALIX PMID: 26859355
homologous domain of human Bro1 domain-containing proteins, Alix and Brox, binds CHMP4B but not STAM2, despite their high structural similarity PMID: 26866605
Accordingly, ALIX depletion leads to furrow regression in cells with chromosome bridges, a phenotype associated with abscission checkpoint signaling failure. PMID: 26929449
Findings indicate that the PDCD6IP 15bp insertion/deletion polymorphism decreases the risk of breast neoplasm in an Iranian population. PMID: 26063962
The serum lever of Alzheimer's disease were decrease and the expression of ALIX strongly correlated with the Mini-Mental State Examination scores of the AD patients PMID: 25502766
our findings identify heparanase as a modulator of the syndecan-syntenin-ALIX pathway, fostering endosomal membrane budding and the biogenesis of exosomes by trimming the heparan sulfate chains on syndecans PMID: 25732677
Our data reveal that AIP1, by inhibiting VEGFR2-dependent signaling in tumor niche, suppresses tumor EMT switch, tumor angiogenesis, and tumor premetastatic niche formation to limit tumor growth and metastasis. PMID: 26139244
Lack of ALG-2, ALIX or Vps4B each prevents shedding, and repair of the injured cell membrane PMID: 25534348
Alix is critically involved in multivesicular body sorting of membrane receptors in mammalian cells. PMID: 25510652
Aip1 has a role in actin filament severing by cofilin and regulates constriction of the cytokinetic contractile ring PMID: 25451933
ALIX is recruited to the neck of the assembling HIV-1 virion and is mostly recycled after virion release. PMID: 24834918
Results suggest that programmed cell death 6 interacting protein (PDCD6IP) insertion/deletion polymorphism was potentially related to non-small cell lung cancer (NSCLC) susceptibility in Chinese Han population. PMID: 24870593
HIV-1 Nef interacts with Alix in late endosomes, and this is required for efficient lysosomal targeting of CD4. PMID: 25118280
Syntenin-ALIX exosome biogenesis and budding into multivesicular bodies are controlled by ARF6 and PLD2. PMID: 24637612
Alix protein plays a critical role in the maintenance of the barrier function of T84 monolayers PMID: 24712823
ALIX regulates these mammalian cell-specific cytokinesis, exosome release, and virus budding. [Review] PMID: 24287454
The results of in vitro binding assays using purified recombinant proteins indicated that ALG-2 functions as a Ca(2)-dependent adaptor protein that bridges ALIX and ESCRT-I to form a ternary complex PMID: 23924735
Common genetic variations in PDCD6IP may influence hepatocellular carcinoma risk, possibly through promoter activity-mediated regulation. PMID: 23777424
Alix serves as an adaptor that allows human parainfluenza virus type 1 to interact with the host cell ESCRT system. PMID: 23527201
Lysobisphosphatidic acid recruits ALIX onto late endosomes via the calcium-bound Bro1 domain, triggering a conformational change in ALIX to mediate the delivery of viral nucleocapsids to the cytosol during infection. PMID: 23664863
Data indicate that AP-3 facilitates PAR1 interaction with ALIX. PMID: 22833563
study reports that the V domain of ALIX binds directly and selectively to K63-linked polyubiquitin chains, exhibiting a strong preference for chains composed of more than three ubiquitins PMID: 23201121
BFRF1 recruits the ESCRT components to modulate nuclear envelope for the nuclear egress of Epstein Barr virus. PMID: 22969426
At the midbody, BRCA2 influences the recruitment of endosomal sorting complex required for transport (ESCRT)-associated proteins, Alix and Tsg101, and formation of CEP55-Alix and CEP55-Tsg101 complexes during abscission. PMID: 22771033
Structural recognition mechanisms between human Src homology domain 3 (SH3) and ALG-2-interacting protein X (Alix PMID: 22641034
Identify key role for syndecan-syntenin-ALIX in membrane transport and signalling processes. PMID: 22660413
structural analysis of the Bro1 domain protein BROX and functional analyses of the ALIX Bro1 domain in HIV-1 budding PMID: 22162750
Mutation of residues within the Phe105 loop of the Bro1 domain compromise Alix function in HIV-1 release. PMID: 21889351
Mutations designed to destabilize the closed conformation of the V domain opened the V domain, increased ALIX membrane association, and enhanced HIV-1 budding. PMID: 21715492
Data suggest that the boomerang-shaped Bro1 domain of Alix appears to escort hepatitis B virus naked capsids without ESCRT. PMID: 21129143
HIV-1 infection affects the expression of host factors TSG101 and Alix PMID: 21528537
The authors demonstrate that ALIX/AIP1, an ESCRT-associated host protein, is required for the incorporation of the nucleoprotein of Mopeia virus, a close relative of Lassa virus, into Z-induced virus-like particles (VLPs). PMID: 21248028
Crystal structures revealed that anchoring tyrosines and nearby hydrophobic residues contact the ALIX V domain, revealing how SIV gag proteins employ a diverse family of late-domain sequences to bind ALIX and promote virus budding. PMID: 20962096
studies on intramolecular interactions:the relieving of specific, autoinhibitory interactions within ALIX regulates binding with ESCRT proteins or viral proteins and is critical for ALIX to participate in retroviral budding PMID: 20929444
Examined changes in subcellular proteomes of different cellular compartments of human endothelial cells upon DENV2 infection. Double immunofluorescence staining revealed colocalization of Alix with late endosomal lysobisphosphatidic acid (LBPA). PMID: 20669987
Identification and biophysical assessment of the molecular recognition mechanisms between the human haemopoietic cell kinase Src homology domain 3 and ALG-2-interacting protein X PMID: 20670214
inability of the two-residue shorter ALG-2 isoform to bind Alix PMID: 20691033
The results indicate YLDL motif in M protein is essential for efficient budding in the context of virus infection and suggest involvement of Alix/AIP1 in Sendai virus budding. PMID: 20605035
Together these data support a model in which Alix recruits Nedd4-1 to facilitate HIV-1 release mediated through the LYPX(n)L/Alix budding pathway via a mechanism that involves Alix ubiquitination. PMID: 20519395
p95 has roles in regulating cell adhesion and morphology. PMID: 12360406
AIP1/Alix interacts with the apoptosis-linked protein ALG-2 and recognizes recognize the protein-protein binding motif YPXL/I, where Tyr, Pro, and Leu/Ile are crucial for its interactive properties PMID: 12588984

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Subcellular Location

Cytoplasm, cytosol. Melanosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Secreted, extracellular exosome. Cell junction, tight junction. Midbody, Midbody ring.

Database Links

HGNC: 8766

OMIM: 608074

KEGG: hsa:10015

STRING: 9606.ENSP00000411825

UniGene: Hs.475896

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Code	CSB-RA568053A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: L02 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, U-87 whole cell lysate, Rat Liver whole cell lysate All lanes: ALIX antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 97, 97, 31 kDa Observed band size: 97 kDa Overlay histogram showing Jurkat cells stained with CSB-RA568053A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/110⁶ cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1µg/110⁶ cells) used under the same conditions. Acquisition of >10,000 events was performed.
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PDCD6IP Recombinant Monoclonal Antibody

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