Recombinant Human Neural cell adhesion molecule L1(L1CAM),partial (Active)

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Code CSB-MP012704HU1
Size $428
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized L1CAM at 2 μg/ml can bind Anti-L1CAM Rabbit Monoclonal Antibody (CSB-RA595071A0HU), the EC50 is 5.384-9.380 ng/ml. Biological Activity Assay
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Product Details

Description

Human neural cell adhesion molecule L1 (L1CAM) amino acids 20-1120 carrying an N-terminal 10xHis-tag and a C-terminal Myc-tag were expressed in mammalian cells. The product is the recombinant human L1CAM protein. This L1CAM protein is characterized by high purity (>95%, SDS-PAGE) and low endotoxin (1.0 EU/ug protein, LAL method). It is an active protein. Its activity was determined via a functional ELISA by binding to the anti-L1CAM rabbit monoclonal antibody, with EC50 values of 5.384-9.380 ng/ mL. It is available now.

L1CAM mainly functions during neural development, including the regulation of neurite outgrowth, fasciculation, cell adhesion, cell migration, myelination, and cell survival. It is also involved in cancer development and is related to metastases and poor prognosis.

Purity Greater than 95% as determined by SDS-PAGE.
Endotoxin Less than 1.0 EU/ug as determined by LAL method.
Activity Measured by its binding ability in a functional ELISA. Immobilized L1CAM at 2 μg/ml can bind Anti-L1CAM Rabbit Monoclonal Antibody (CSB-RA595071A0HU), the EC50 is 5.384-9.380 ng/ml.
Target Names L1CAM
Uniprot No. P32004
Alternative Names (CD171)(CAML1)(MIC5)
Molecular Characterization
Species Homo sapiens (Human)
Source Mammalian cell
Expression Region 20-1120aa
Target Protein Sequence IQIPEEYEGHHVMEPPVITEQSPRRLVVFPTDDISLKCEASGKPEVQFRWTRDGVHFKPKEELGVTVYQSPHSGSFTITGNNSNFAQRFQGIYRCFASNKLGTAMSHEIRLMAEGAPKWPKETVKPVEVEEGESVVLPCNPPPSAEPLRIYWMNSKILHIKQDERVTMGQNGNLYFANVLTSDNHSDYICHAHFPGTRTIIQKEPIDLRVKATNSMIDRKPRLLFPTNSSSHLVALQGQPLVLECIAEGFPTPTIKWLRPSGPMPADRVTYQNHNKTLQLLKVGEEDDGEYRCLAENSLGSARHAYYVTVEAAPYWLHKPQSHLYGPGETARLDCQVQGRPQPEVTWRINGIPVEELAKDQKYRIQRGALILSNVQPSDTMVTQCEARNRHGLLLANAYIYVVQLPAKILTADNQTYMAVQGSTAYLLCKAFGAPVPSVQWLDEDGTTVLQDERFFPYANGTLGIRDLQANDTGRYFCLAANDQNNVTIMANLKVKDATQITQGPRSTIEKKGSRVTFTCQASFDPSLQPSITWRGDGRDLQELGDSDKYFIEDGRLVIHSLDYSDQGNYSCVASTELDVVESRAQLLVVGSPGPVPRLVLSDLHLLTQSQVRVSWSPAEDHNAPIEKYDIEFEDKEMAPEKWYSLGKVPGNQTSTTLKLSPYVHYTFRVTAINKYGPGEPSPVSETVVTPEAAPEKNPVDVKGEGNETTNMVITWKPLRWMDWNAPQVQYRVQWRPQGTRGPWQEQIVSDPFLVVSNTSTFVPYEIKVQAVNSQGKGPEPQVTIGYSGEDYPQAIPELEGIEILNSSAVLVKWRPVDLAQVKGHLRGYNVTYWREGSQRKHSKRHIHKDHVVVPANTTSVILSGLRPYSSYHLEVQAFNGRGSGPASEFTFSTPEGVPGHPEALHLECQSNTSLLLRWQPPLSHNGVLTGYVLSYHPLDEGGKGQLSFNLRDPELRTHNLTDLSPHLRYRFQLQATTKEGPGEAIVREGGTMALSGISDFGNISATAGENYSVVSWVPKEGQCNFRFHILFKALGEEKGGASLSPQYVSYNQSSYTQWDLQPDTDYEIHLFKERMFRHQMAVKTNGTGRVRLPPAGFATE
Mol. Weight 128.2 kDa
Protein Length Partial
Tag Info N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time 3-7 business days
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Function
Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity.
Gene References into Functions
  1. In uterine carcinosarcoma, membranous L1CAM expression was positive in the epithelial component in 65.4% of cases. Remarkably, expression was negative in the mesenchymal component. In cases where both components were intermingled, expression limited to the epithelial component was confirmed by a double stain for L1CAM and keratin. Expression of L1CAM did not relate to overall or disease-free survival. PMID: 30140948
  2. Our findings suggest that L1CAM is possibly involved in the pathogenesis of at least a subset of endometrial clear cell carcinomas PMID: 28941294
  3. The directional force for laminin-induced growth cone haptotaxis is generated by the grip and slip of L1-CAM on the substrates, which occur asymmetrically under the growth cone. PMID: 29483251
  4. L1CAM promotes esophageal squamous cell carcinoma tumorigenicity by upregulating ezrin expression. PMID: 28939985
  5. TWIST1, in part via GAS6 and L1CAM, led to higher expression and activation of Akt upon cisplatin treatment, and inhibition of Akt activation sensitized cells to cisplatin. PMID: 27876874
  6. data make L1CAM a highly interesting therapeutic target to prevent further metastatic spread in melanoma patients PMID: 29432466
  7. High circulating levels of autoantibodies against L1-cell adhesion molecule is associated with esophageal squamous cell carcinoma. PMID: 28181176
  8. A functional role for L1CAM in extrahepatic cholangiocarcinoma carrying the activating KRAS mutation.L1CAM prmotes cell migration and invasion via JNK activation in extrahepatic cholangiocarcinoma. PMID: 28535665
  9. this review and meta-analysis concludes that L1CAM might be an effective poor prognostic factor for patients with various tumor types PMID: 27833079
  10. High L1CAM expression is associated with vulvar squamous cell carcinomas. PMID: 27028855
  11. Our preclinical assessment of the CE7 epitope on CD171 supports its utility and safety as a CAR T-cell target for neuroblastoma immunotherapy PMID: 27390347
  12. L1CAM may have a role in human endometrial cancer and miR-34a has an inverse role to L1CAMEXP PMID: 27233077
  13. L1CAM mRNA expression appears to play a substantial role in the pathophysiology of ovarian cancer that is translated into poor clinical outcome. PMID: 27174921
  14. These results suggest that a deficiency in L1 may partially account for RTT phenotypes. PMID: 29050935
  15. L1CAM failed to be a clinically relevant marker of poor prognosis in stage I endometrioid endometrial carcinoma PMID: 27488577
  16. This study revealed an unexpected role of L1CAM in the pathological crosstalk between the immune and nervous systems. PMID: 27544757
  17. Mutations involving L1 cell adhesion molecule is associated with chemotherapy-resistant urothelial carcinoma. PMID: 27749842
  18. Data suggest that targeted therapy to neural cell adhesion molecule L1 (L1) might be effective in the treatment of retinoblastoma tumors. PMID: 28061460
  19. CD10 is a necessary component conferring the L1 effects in CRC cells. The identification of gene expression patterns of L1-domain-specific point mutations may provide novel markers and targets for interfering with L1-mediated CRC progression. PMID: 27641335
  20. High L1-CAM expression is associated with low radiosensitivity in neuroblastoma. PMID: 27432152
  21. Neural cell adhesion molecule L1 (L1CAM)-mediated cell-cell aggregation was severely impaired by L1CAM variants p.I37N, p.M172I and p.D202Y but was preserved by the variant p.T38M. PMID: 26891472
  22. The differential expression timing of CD184 and CD171 permits identification and enrichment of RGCs from retinal organoids at differing maturation states from committed progenitors to differentiating neurons. PMID: 27867005
  23. This study examined the spatiotemporal distribution of L1CAM in the early human fetal period by means of immunohistochemistry and in situ hybridization. In advanced differentiated epithelia such as those of the gastrointestinal system, L1CAM localization vanishes. In epithelia, however, which undergo further development such as those of the urogenital system, L1CAM is further needed for their fully establishment. PMID: 28026654
  24. The expression of L1CAM was associated with advanced stage, nodal involvement, high tumour grade, non-endometrioid histology, lymphovascular space invasion, and distant recurrences in all cases, and with reduced survival in the endometrial carcinomas, but not in the non-endometrioid carcinomas. L1CAM may induce EMT-like changes, but seems to only play a role in metastasis, not in invasion. PMID: 27505134
  25. L1CAM expression is an independent predictor of poor survival in endometrial cancer, and is associated with advanced stage, high-risk endometrial cancer. PMID: 26861585
  26. L1CAM is a neuronal cell adhesion molecule involved in the development of the nervous system and progression of malignancies. (Review) PMID: 27267927
  27. Splicing variant c.1267+5delG was identified in fetal hydrocephalus. The same mutation and severe L1 syndrome was confirmed in the second pregnancy. PMID: 27207492
  28. Involvement of L1CAM in the regulation of activity of the canonical Wnt pathway and expression of genes of class I melanoma-associated antigens in melanoma. PMID: 27165065
  29. L1CAM was a significant independent prognosticator for disease-specific survival in endometrial carcinoma. PMID: 27695947
  30. Report high frequency of L1CAM expression in high-risk endometrial cancers associated with mutant p53 expression. PMID: 26743472
  31. L1CAM is frequently expressed in testicular germ cell tumors but not in normal testis. PMID: 26933044
  32. L1 syndrome should be considered in the differential diagnosis of intellectual disability or mental retardation in children, especially when other signs such as hydrocephalus or adducted thumbs are present. PMID: 25948108
  33. Genes induced during L1-mediated colorectal cancer cell metastasis PMID: 26399194
  34. Our results suggest that the overexpression of L1CAM may be related to several established markers of poor prognosis in breast cancer patients. PMID: 26464672
  35. L1-CAM and N-CAM: From Adhesion Proteins to Pharmacological Targets PMID: 26478212
  36. the CE7-epitope of L1-CAM is a cell adhesion molecule aberrantly expressed in several cancers and may have a role in immunotherapy PMID: 26761817
  37. novel missense variant in L1CAM was identified in two Caucasian families with mild-moderate intellectual disability without obvious L1 syndrome features PMID: 25934484
  38. This study identified predicted pathogenic, hemizygous variants on chromosome X in disease genes L1CAM. PMID: 25666757
  39. Our findings establish Slug-induced L1CAM expression as a mediator of a chemoresistant and migratory phenotype in pancreatic adenocarcinoma cells. PMID: 25860483
  40. the expression level of L1CAM were negatively correlated with miR-503 levels in osteosarcoma tissues. PMID: 25536034
  41. L1CAM is expressed in triple-negative breast cancers and is inversely correlated with androgen receptor PMID: 25510351
  42. a positive relationship between L1 and pPKD1 in both cultured cerebellar neurons and human cerebellar tissue, suggesting that L1 functions in the modulation of PKD1 phosphorylation. PMID: 25445362
  43. This indicates that similar biofunctionalization approaches based on N-cadherin and L1 can be translated to 3-D "transplantable" scaffolds with enhanced neurotrophic behaviors. PMID: 24914828
  44. Findings shed new light on the complex regulation of L1CAM in cancers and advocate the use of L1CAM/miR-21-3p for diagnostic application. PMID: 25149066
  45. The data show that L1CAM promotes the enrichment of immunosuppressive T cells in particular of a CD4(+)CD25(-)CD69(+)-phenotype in pancreatic ductal adenocarcinoma providing a novel mechanism of tumor immune escape which contributes to tumor progression. PMID: 24746181
  46. Data suggest that miR-34a can regulate L1CAM expression by targeting L1CAM mRNA for degradation. PMID: 24497324
  47. Data indicate that positive L1 cell adhesion molecule (L1CAM) expression was significantly correlated with risk of distant recurrence. PMID: 25126672
  48. Overexpression of L1CAM is associated with tumor progression via ERK signaling in gastric cancer. PMID: 24046108
  49. Expression of L1CAM and EPCAM in gastric cancer was significantly associated with lymph node and distant metastasis, and poor prognosis. PMID: 24422715
  50. Human pathological H210Q, R184Q and Y1070C, but not the E309K and L120V L1CAM mutations affect outside-in signaling via the FIGQY Ankyrin binding domain which is required for synapse formation. PMID: 24155914

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Involvement in disease Hydrocephalus due to stenosis of the aqueduct of Sylvius (HSAS); Mental retardation, aphasia, shuffling gait, and adducted thumbs syndrome (MASA); Agenesis of the corpus callosum, X-linked, partial (ACCPX)
Subcellular Location Cell membrane; Single-pass type I membrane protein. Cell projection, growth cone. Cell projection, axon. Cell projection, dendrite.
Protein Families Immunoglobulin superfamily, L1/neurofascin/NgCAM family
Database Links

HGNC: 6470

OMIM: 303350

KEGG: hsa:3897

STRING: 9606.ENSP00000359074

UniGene: Hs.522818

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