L1CAM

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized L1CAM at 2 μg/ml can bind Anti-L1CAM Rabbit Monoclonal Antibody (CSB-RA595071A0HU), the EC₅₀ is 5.384-9.380 ng/ml.

IHC image of CSB-RA588962A0HU diluted at 1:50 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.76% DAB.

Overlay Peak curve showing MCF-7 cells stained with CSB-RA588962A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Western Blot
Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate
All lanes: L1CAM antibody at 2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 141, 140, 139 kDa
Observed band size: 90 kDa

Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA07439A0Rb at dilution of 1:100

Immunofluorescent analysis of A549 cells using CSB-PA07439A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)