Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
①Measured by its binding ability in a functional ELISA. Immobilized Mouse CADM1 at 2 μg/mL can bind Mouse CRTAM (CSB-MP614820MO), the EC50 is 52.61-92.92 ng/mL.
Alternative Names
(Immunoglobulin superfamily member 4)(Nectin-like protein 2 )(Spermatogenic immunoglobulin superfamily)(Synaptic cell adhesion molecule)(Tumor suppressor in lung cancer 1)
Species
Mus musculus (Mouse)
Expression Region
48-388aa
Target Protein Sequence
QNLFTKDVTVIEGEVATISCQVNKSDDSVIQLLNPNRQTIYFRDFRPLKDSRFQLLNFSSSELKVSLTNVSISDEGRYFCQLYTDPPQESYTTITVLVPPRNLMIDIQKDTAVEGEEIEVNCTAMASKPATTIRWFKGNKELKGKSEVEEWSDMYTVTSQLMLKVHKEDDGVPVICQVEHPAVTGNLQTQRYLEVQYKPQVHIQMTYPLQGLTREGDAFELTCEAIGKPQPVMVTWVRVDDEMPQHAVLSGPNLFINNLNKTDNGTYRCEASNIVGKAHSDYMLYVYDPPTTIPPPTTTTTTTTTTTTTILTIITDTTATTEPAVHDSRAGEEGTIGAVDH
Tag Info
C-terminal 10xHis-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA
Please contact us to get it.
Description
This Human Cadm1 recombinant protein was produced in Mammalian cell, where the gene sequence encoding Human Cadm1 (48-388aa) was expressed with the C-terminal 10xHis-tag. The purity of this Cadm1 protein was greater than 95% by SDS-PAGE. Its endotoxin content is less than 1.0 EU/ug as determined by LAL method. The activity was validated. In a functional ELISA, immobilized mouse CADM1 at 2 μg/mL can bind Mouse CRTAM (CSB-MP614820MO) with the EC50 of 52.61-92.92 ng/mL.
CADM1 (Cell adhesion molecule 1) was originally discovered and characterized by multiple research groups. As a result, the molecule has many names in the scientific literature, including cell adhesion molecule 1 (CADM1), synaptic cell adhesion molecule (synCAM), biogenic Sperm immunoglobulin superfamily molecules (sgIGSF), IGSF4, BL2, ST17, NECL2, RA175, and CADM1A. CADM1 is a glycoprotein located on the cell surface. It was initially identified as a tumor suppressor gene through functional complementation experiments in human non-small cell lung cancer. At present, a large amount of evidence shows that CADM1 is involved in the formation and development of many human tumors. CADM1 was initially identified as a tumor suppressor, and accumulating evidence suggests that downregulation of CADM1 contributes to tumorigenesis in various cancers, but its mechanism of action requires further investigation. The development of active and stable CADM1 protein can facilitate the further exploration of related mechanisms and provide new clues for tumor monitoring, diagnosis and treatment.
