CTCF Antibody

Code CSB-PA006138DSR2HU
Size US$167Purchase it in Cusabio online store
(only available for customers from the US)
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  • Western blot
    All lanes: Transcriptional repressor CTCF antibody at 6μg/ml
    Lane 1: PC-3 whole cell lysate
    Lane 2: MCF-7 whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 83, 46 kDa
    Observed band size: 83 kDa

  • Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA006138DSR2HU at dilution of 1:100

  • Chromatin Immunoprecipitation Hela (1.2*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4μg anti-CTCF or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers (CSB-PP006138HU) against the H19ICR promoter.

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) CTCF Polyclonal antibody
Uniprot No. P49711
Target Names CTCF
Alternative Names 11 zinc finger protein antibody; 11 zinc finger transcriptional repressor antibody; 11-zinc finger protein antibody; CCCTC binding factor (zinc finger protein) antibody; CCCTC binding factor antibody; CCCTC-binding factor antibody; Ctcf antibody; CTCF_HUMAN antibody; CTCFL paralog antibody; MRD21 antibody; Transcriptional repressor CTCF antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Transcriptional repressor CTCF protein (1-260AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Antigen Affinity Purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form Liquid
Tested Applications ELISA, WB, IHC, ChIP
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IHC 1:20-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. Seems to act as tumor suppressor. Plays a critical role in the epigenetic regulation. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Plays a critical role in gene silencing over considerable distances in the genome. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays a important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping. Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription. When bound to chromatin, it provides an anchor point for nucleosomes positioning. Seems to be essential for homologous X-chromosome pairing. May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation. May play a role in preventing the propagation of stable methylation at the escape genes from X- inactivation. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites. Regulates asynchronous replication of IGF2/H19. Plays a role in the recruitment of CENPE to the pericentromeric/centromeric regions of the chromosome during mitosis
Gene References into Functions
  1. The authors propose that cellular CCCTC-binding factor binding at the herpes simplex virus 1 CCCTC-binding factor binding sites (CTRL2) acts as a chromatin insulator to keep viral chromatin in a form that is poised for reactivation, a state which we call poised latency. PMID: 29437926
  2. neither the deletion of the CTCF locus nor the ectopic insertion of Firre cDNA or its ectopic expression are sufficient to alter topologically associated domains in a sex-specific or allele-specific manner PMID: 29654311
  3. CTCF maintains regulatory homeostasis of cancer pathways. PMID: 30086769
  4. these results suggest that CTCF participate in DNA damage response via poly(ADP-ribosylation). PMID: 28262757
  5. Studies suggest that the connection between DNA-binding protein CTCF (CTCF), cohesin, chromatin structure, and behavior is important in understanding of the development of behavior in general, and neurodevelopmental disorders in particular [Review]. PMID: 29110030
  6. ID1, CTCF and ELK1 may be associated with prostate cancer, and may be potential therapeutic targets for the treatment of this disease. PMID: 29956775
  7. CTCF promotes HSV-1 lytic transcription by facilitating the elongation of RNA Pol II and preventing silenced chromatin on the viral genome. PMID: 28045091
  8. These results, together with a prior exomesequencing based study, suggest that CTCF mutations may be involved in the development of ovarian endometriosis. PMID: 29845264
  9. findings establish for the first time that CTCF is an important regulator of the homologous recombination repair pathway. PMID: 28560323
  10. Findings indicate that CCCTC-binding factor (CTCF)-driven doublesex and mab-3 related transcription factor 2 protein (TERRA) transcription acts in cis to facilitate telomere repeat replication and chromosome stability. PMID: 29235471
  11. poly(ADP-ribosyl)ated CTCF changes its DNA binding and localisation in a breast cell line which is associated with nucleosome repositioning. PMID: 29981477
  12. Here, we show that PARP1 and host insulator protein CTCF colocalize at specific sites throughout the EBV genome and provide evidence to suggest that PARP1 acts to stabilize CTCF binding and maintain the open chromatin landscape at the active Cp promoter during type III latency. Further, PARP1 activity is important in maintaining latency type-specific viral gene expression. PMID: 29976663
  13. HOTTIP cooperates with CTCF to coordinate HOXA gene expression. PMID: 29698677
  14. CD4(+) T cells showed the greatest increase (threefold) in ORMDL3 expression in individuals carrying the asthma-risk alleles, where ORMDL3 negatively regulated interleukin-2 production. The asthma-risk variants rs4065275 and rs12936231 switched CTCF-binding sites in the 17q21 locus. PMID: 27848966
  15. Our data reveal that vigilin is essential for maintenance of imprinting of IGF2 gene via functional interaction between KH1-7 domains of vigilin and zinc-finger domains of CTCF. PMID: 29157910
  16. This study confirms that haploinsufficiency of CTCF causes distinct clinical features, and that a microdeletion encompassing CTCF could cause a recognisable CTCF deletion syndrome. Perturbed DNA methylation at CTCF binding sites, not at imprinted loci, may underlie the pathomechanism of the syndrome. PMID: 28848059
  17. structural studies show that the sequence-specific interactions between zinc fingers and CTCF-binding sites determine the directionality and conservation of CTCF recognition. PMID: 29076501
  18. CTCF may be a key factor that contributes to gene co-mutations in cancer. PMID: 27762310
  19. results support a model in which YY1 acts as an architectural protein to connect developmentally regulated looping interactions; the location of YY1-mediated interactions may be demarcated in development by a preexisting topological framework created by constitutive CTCF-mediated interactions. PMID: 28536180
  20. The MeCP2, a protein whose mutated forms are involved in Rett syndrome; and CTCF, a constitutive transcriptional insulator. PMID: 28796949
  21. The results show that cohesin has an essential genome-wide function in mediating long-range chromatin interactions and support the hypothesis that cohesin creates these by loop extrusion, until it is delayed by CTCF in a manner dependent on PDS5 proteins, or until it is released from DNA by WAPL. PMID: 29217591
  22. CTCF-FOXM1 axis regulates tumour growth and metastasis in hepatocellular carcinoma cells. PMID: 28862757
  23. we show CTCF binding site mutations to be functional by demonstrating allele-specific reduction of CTCF binding to mutant alleles. While topologically associating domains with mutated CTCF anchors in melanoma contain differentially expressed cancer-associated genes, CTCF motif mutations appear generally under neutral selection PMID: 27974201
  24. CTCF-mediated long-range interactions are integral for a multitude of topological features of interphase chromatin, such as the formation of topologically associated domains, domain insulation, enhancer blocking and even enhancer function. PMID: 26802288
  25. Authors have identified two novel pro-tumorigenic roles (promoting cell survival and altering cell polarity) for genetic alterations of CTCF in endometrial cancer. PMID: 28319062
  26. Describe several protein-DNA complex structures of a human CTCF tandem zinc-finger array, explaining the adaptability of CTCF to sequence variations and the positiondependent effect of differential DNA methylation at two cytosine residues, and revealing a potential function of C-terminal ZF8 and ZF9 spanning across the DNA phosphate backbone. PMID: 28529057
  27. CCCTC-binding factor (CTCF) targets the binding sites within MYCN promoter to facilitate its expression in neuroblastoma (NB) cells. PMID: 26549029
  28. we review recent high-resolution chromosome conformation capture and functional studies that have informed models of the spatial and regulatory compartmentalization of mammalian genomes, and discuss mechanistic models for how CTCF and cohesin control the functional architecture of mammalian chromosomes. PMID: 27089971
  29. GAD1 is reactivated by DNA methylation, which provided a model for DNA methylation and the active orchestration of oncogenic gene expression by CTCF in cancer cells. PMID: 26549033
  30. epigenetic factor CTCF-mediated chromatin remodeling regulates interactions between eye-specific PAX6 and those genes that are induced/associated with cell differentiation to modulate corneal epithelial cell-specific differentiation PMID: 27583466
  31. Rta-mediated decreased binding of CTCF in the viral genome is concurrent with virus reactivation. Via interfering with CTCF binding, in the host genome Rta can function as a transcriptional repressor for gene silencing. PMID: 28490592
  32. although we were unable to detect HD-associated DNA methylation alterations at queried sites, we found that DNA methylation may be correlated to the age of disease onset in cortex tissues. Moreover, our data suggest that DNA methylation may, in part, contribute to tissue-specific HTT transcription through differential CTCF occupancy. PMID: 26953320
  33. vitamin D-sensitive CTCF sites provide further mechanistic details to the epigenome-wide understanding of 1,25(OH)2D3-mediated gene regulation PMID: 27569350
  34. These findings indicate that erythroid specific activator GATA-1 acts at CTCF sites around the beta-globin locus to establish tissue-specific chromatin organization. PMID: 28161276
  35. TOP2B is positioned to solve topological problems at diverse cis-regulatory elements and its occupancy is a highly ordered and prevalent feature of CTCF/cohesin binding sites that flank TADs. PMID: 27582050
  36. CTCF binding to eRNAs and promoters is facilitated by estrogen when chromatin establishes contacts with nuclear lamina. PMID: 27638884
  37. we investigated the cell-type specificities of CTCF sites related to these functions across five cell types. Our study provides new insights into the multivalent functions of CTCF in the human genome PMID: 27067545
  38. The results indicate that the initial chromatin conformation affects subsequent RA-induced HOXA gene activation. Our study uncovers that a removable insulator spatiotemporally switches higher-order chromatin and multiple gene activities via cooperation of CTCF and key transcription factors. PMID: 27798106
  39. we find no evidence for selection driving these distinctive patterns of mutation. The mutational load at CTCF-binding sites is substantially determined by replication timing and the mutational signature of the tumor in question, suggesting that selectively neutral processes underlie the unusual mutation patterns. PMID: 27490693
  40. data demonstrate a reciprocal relationship between Hmgb2 and Ctcf in controlling aspects of chromatin structure and gene expression. PMID: 27226577
  41. Study described the formation of mutually exclusive complexes of ENY2 with insulator proteins and Sgfl1-a component of the SAGA complex, direct binding partner for ENY2 PMID: 27417714
  42. This is supported by the depletion of CTCF in glioblastoma cells affecting the expression levels of NOTCH2 as a target of miR-181c. CONCLUSION: Together, our results point to the epigenetic role of CTCF in the regulation of microRNAs implicated in tumorigenesis PMID: 26983574
  43. Our data show that aberrant epigenetic inactivation of DUSP2 occurs in carcinogenesis and that CTCF is involved in the epigenetic regulation of DUSP2 expression. PMID: 26833217
  44. Mutational analysis highlighted a potential role for CTCF, a crucial regulator of long-range chromatin interactions, in head and neck cancer progression. PMID: 26747525
  45. CTCF and cohesins shape the genome during evolution. (Review) PMID: 26439501
  46. The action of SNF2H at CTCF sites is functionally important as depletion of CTCF or SNF2H affects transcription of a common group of genes. PMID: 27019336
  47. CSB and CTCF can regulate each other's chromatin association, thereby modulating chromatin structure and coordinating gene expression in response to oxidative stress. PMID: 26578602
  48. CTCF binds to the provirus at a sharp border in epigenetic modifications in the pX region of the HTLV-1 provirus in T cells naturally infected with HTLV-1. This may cause widespread abnormalities in host cell chromatin structure and gene expression. PMID: 26929370
  49. CTCF/cohesin coordinates HOXA cluster higher-order chromatin structure and expression during development PMID: 26376810
  50. CTCF has a role in regulating SLC45A3-ELK4 Chimeric RNA PMID: 26938874
  51. TGF-beta/beta2-spectrin/CTCF-regulated tumor suppression in human stem cell disorder Beckwith-Wiedemann syndrome. PMID: 26784546
  52. CTCF helps recruit CENP-E to the centromere during mitosis and that it may do so through a structure stabilized by the CTCF/CENP-E complex. PMID: 26321640
  53. Here, the authors reveal the methylcytosine dioxygenases TET1 and TET2 as active regulators of CTCF-mediated alternative splicing through conversion of 5-methylcytosine to its oxidation derivatives. PMID: 26711177
  54. These results demonstrate the existence of definitive CTCF binding motifs corresponding to CTCF's diverse functions. PMID: 26315019
  55. the CTCF-associated boundary element, CBS5, employs both Cohesin and noncoding RNA to establish and maintain topologically associated domains at the HOXA locus. PMID: 26302455
  56. CTCF negatively regulates HOXA10 expression in breast cancer cells.CTCF flanks important promoter element of HOXA10. PMID: 26478432
  57. Study suggests that genome-wide topological chromatin looping can be predicted based on CTCF/cohesin directional binding; findings reveal how nonlinear 3D genome topology could be encoded by linear 1D genomic sequences. PMID: 26276636
  58. CTCF can both enhance and repress virus gene expression. [review] PMID: 26154016
  59. 3D genome simulation suggests a model of chromatin folding around chromosomal axes, where CTCF is involved in defining the interface between condensed and open compartments for structural regulation. PMID: 26686651
  60. Data found that BORIS and CTCF expression in low-grade squamous intraepithelial lesions and invasive cervical carcinoma is higher than in normal samples. The possible utility of BORIS and CTCF as biomarkers in cervical neoplasm requires further analysis. PMID: 26125810
  61. Our findings identify both SMC1 and CTCF as critical regulators of the differentiation-dependent life cycle of high-risk human papillomaviruses PMID: 25875106
  62. results provide evidence that CTCF/cohesin-mediated chromatin looping of the PTGS2 locus is dynamically influenced by the DNA methylation status. PMID: 25703332
  63. Results provide insights into the distribution of histone modifications at promoters and suggest a novel role of CTCF and cohesin as regulators of transcriptional direction. PMID: 25881024
  64. A novel single nucleotide polymorphism rs60507107 was significantly associated with increased risk of lung cancer in two stages PMID: 25592173
  65. These elements are highly occupied by the insulator protein CTCF, are DNase I hypersensitive and represent only a small minority of the CTCF recognition sequences in the human genome PMID: 25580597
  66. PARP1- and CTCF-regulate contacts between circadian loci and the repressive chromatin environment at the lamina therefore mediate circadian transcriptional plasticity. PMID: 26321255
  67. Studied the role of looping in large-scale (supra Mb) folding of human chromosomes by knocking down the gene that codes for CTCF and the one coding for Rad21, an essential subunit of cohesin. PMID: 25299688
  68. CTCF controls the homeostatic maintenance and migration of Langerhans cells. PMID: 25936568
  69. our data reveal extensive genetic effects on CTCF binding, both direct and indirect, and identify a diversity of patterns of CTCF binding on the X chromosome. PMID: 25411781
  70. data demonstrate there is strong selection for inactivation of both CTCF and ZFHX3 in Endometroid Endometrial cancer(EEC). PMID: 26330387
  71. a considerable number of alternative splicing events could have a chromatin-dependent regulation involving the association of HP1alpha and CTCF near regulated exons. PMID: 25934638
  72. In the first detailed analysis in cancer, a marked loss of CHD8 expression and increased BORIS/CTCF ratio indicate frequent disruption of CTCF and its effector genes in PCa. PMID: 25499215
  73. To validate this finding and to better understand the phenomenon, we used LNCaP, a prostate cell line as a model, and identified 16 additional cis-SAGe events by silencing transcription factor CTCF and paired-end RNA sequencing. PMID: 25658338
  74. In the gene-based analysis, CACNB2 and CTCF showed the strongest evidence for association with schizophrenia in both the present samples and in those of the Psychiatric Genetics Consortium datasets. PMID: 24901509
  75. Authors conclude that high-risk human papillomavirus types have evolved to recruit CTCF to the early gene region to control the balance and complexity of splicing events that regulate viral oncoprotein expression. PMID: 25694598
  76. This work demonstrates that, in contexts in which BCL6 is expressed, CTCF binding to BCL6 exon1A associates with epigenetic modifications indicative of transcriptionally open chromatin. PMID: 24362533
  77. Findings establish CTCF as a prominent tumor-suppressor gene and point to CTCF-mediated epigenetic stability as a major barrier to neoplastic progression. PMID: 24794443
  78. Results found that CTCF may be bound immediately upstream of RNA polymerase II in most active CpG island promoters. PMID: 23186133
  79. CTCF and Rad21 act as host cell restriction factors for Kaposi's sarcoma-associated herpesvirus (KSHV) lytic replication by modulating viral gene transcription. PMID: 24415941
  80. Data show that CCCTC-binding factor (CTCF) together with ISWI ATPase SMARCA5 and members of the Cohesin complex associate with the SPI1 protein is disrupted in acute myeloid leukemia (AML) blasts. PMID: 24498324
  81. CTCF facilitates the stabilization of Polycomb repressive complex 2 and trimethylated lysine 27 of histone H3 at the human HOXA locus. PMID: 25135475
  82. Our results demonstrated an aberrant methylation pattern at a CTCF binding site downstream the WT1 gene, which is associated with an elevated WT1 transcriptional activity PMID: 24534946
  83. The methylation status of CTCF and SIRT6 promoter regions was not statistically different in cancer lesions compared with matched normal tissues. PMID: 24842653
  84. CTCF may play a significant role in the anti-apoptotic pathway mediated by NF-kappaB in pediatric Acute lymphoblastic leukemia PMID: 24393203
  85. results reveal the functional relevance of vigilin and CTCF, and show that the CTCF-vigilin complex contributes to regulation of Igf2/H19 PMID: 24725430
  86. Data shows that T204fs, a frameshift mutation in CTCF gene is frequent in endometrial neoplasms with microsatellite istability. Also, The spectrum and pattern of mutations observed is consistent with CTCF acting as a haploinsufficient tumor suppressor. PMID: 24130125
  87. Data show that the transcription of angiogenin (ANG) and ribonuclease 4 (RNASE4) promoter is influenced by RNA polymerase III (Pol III) elements and could be differentially regulated by an intragenic CCCTC binding factor (CTCF)-dependent chromatin loop. PMID: 24659782
  88. CTCF may regulate vigilin behavior and thus indirectly influence the binding of HP1alpha to the satellite 2 locus PMID: 24561205
  89. CTCF links the architecture of the genome to its function. [Review] PMID: 24614316
  90. Results show that CCCTC-binding factor (CTCF) plays an important role in maintaining telomerase reverse transcriptase catalytic subunit (TERT) expression in a subset of lung cancer cell lines may be due to CTCF-dependent enhancer-promoter interactions. PMID: 24174344
  91. A single CTCF binding site controls LMP2A and LMP1 promoter selection, chromatin boundary function and episome copy number control during Epstein-Barr virus latency. PMID: 24257606
  92. Increased DNA methylation at the FXN upstream GAA region, primarily 5 hmC rather than 5 mC, and decreased CTCF occupancy at the FXN 5' UTR are associated with FRDA disease-relevant human tissues. PMID: 24023969
  93. CTCF and cohesin contribute differentially to chromatin organization and gene regulation PMID: 24335803
  94. The intronic binding sites for cohesin and CTCF coincide with histone modifications that confer enhancer-like properties, and two of the cohesin/CTCF sites behaved as insulators in an in vivo assay. PMID: 24321385
  95. Data show that CCCTC binding factor (CTCF) binding was enriched at the Bax CTCF binding sites (CTSs) in breast cancer cells and tumors. PMID: 23908591
  96. condensin competes with CTCF in binding to a specific rDNA locus and negatively regulates CTCF-mediated rRNA gene transcription. PMID: 23884423
  97. CTCF regulates the expression of IPO13, which, in turn, mediates the nuclear import of CTCF. PMID: 23620300
  98. FXS cells and its splicing is correlated to that of the FMR1-mRNA. We conclude that CTCF has a complex role in regulating FMR1 expression, probably through the organization of chromatin loops between sense/antisense transcriptional regulatory regions PMID: 23874213
  99. a direct regulatory relationship between EGF-induced NFkappaB p50 and CTCF activation affecting corneal epithelial wound healing has been established PMID: 23843455
  100. CTCF and BORIS suppressed breast cancer growth; findings provide further evidence that CTCF behaves as a tumor suppressor, and show BORIS has a similar growth inhibitory effect in vitro and in vivo PMID: 23553099

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Involvement in disease Mental retardation, autosomal dominant 21 (MRD21)
Subcellular Location Nucleus, nucleoplasm, Chromosome, Chromosome, centromere
Protein Families CTCF zinc-finger protein family
Tissue Specificity Ubiquitous. Absent in primary spermatocytes.
Database Links

HGNC: 13723

OMIM: 604167

KEGG: hsa:10664

STRING: 9606.ENSP00000264010

UniGene: Hs.368367

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