GRB10 Antibody

Code CSB-PA002806
Size US$100
  • Western Blot analysis of 3T3 cells using GRB10 Polyclonal Antibody
  • Western Blot analysis of NIH-3T3 cells using GRB10 Polyclonal Antibody
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Product Details

Uniprot No.
Target Names
Alternative Names
grb 10 antibody; GRB IR antibody; grb-10 antibody; GRB10 adapter protein antibody; GRB10 adaptor protein antibody; GRB10 antibody; GRB10_HUMAN antibody; GRBIR antibody; Growth factor receptor bound protein 10 antibody; Growth factor receptor-bound protein 10 antibody; Insulin receptor binding protein antibody; Insulin receptor binding protein GRB IR antibody; Insulin receptor-binding protein Grb-IR antibody; IRBP antibody; KIAA0207 antibody; Maternally expressed gene 1 antibody; MEG1 antibody; RSS antibody
Raised in
Species Reactivity
Synthesized peptide derived from Human GRB10 around the non-phosphorylation site of Y67.
Immunogen Species
Homo sapiens (Human)
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
It differs from different batches. Please contact us to confirm it.
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Tested Applications
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Troubleshooting and FAQs
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2.
Gene References into Functions
  1. Through syngeneic comparison, our study identifies for the first time that Grb10 is associated with the pluripotency state in nuclear transfer embryonic stem cells. PMID: 28476045
  2. Data suggest that GRB10 and GRB14 are both Ca2+-dependent CaM-binding proteins; more than one CaM-binding site and/or accessory CaM-binding sites appear to exist in GRB10 and GRB14, as compared to a single one present in GRB7. (GRB10 = growth factor receptor-bound protein 10; GRB14 = growth factor receptor-bound protein 14; CaM = calmodulin; GRB7 = growth factor receptor-bound protein 7) PMID: 28295264
  3. GRB10 may regulate degradation of the IL-4 receptor-signaling complex through interactions with NEDD4.2. PMID: 27742835
  4. placental expression associated positively with placental weight, birth weight, and neonatal head circumference PMID: 26390806
  5. Risk alleles for 6 loci increased glucose levels from birth to 5 years of age (ADCY5, ADRA2A, CDKAL1, CDKN2A/B, GRB10, and TCF7L2 PMID: 27049325
  6. This study demonstrated that the most significant SNP (rs11770199; p = 0.0003) in single-site analysis was located on chromosome 7 in the GRB10 gene. PMID: 25391383
  7. Association of the intronic polymorphism rs12540874 A>G of the GRB10 gene with high birth weight. PMID: 25103788
  8. Tissue-specific methylation and possibly imprinting of GRB10 can influence glucose metabolism. PMID: 24699409
  9. Studies indicate that insulin receptor (IR) and IGF Type 1 Receptor (IGFR) have been identified as important partners of Grb10/14 and SH2B1/B2 adaptors. PMID: 23190452
  10. H19 and GRB10 methylation was normal in Albright's hereditary osteodystrophy patients. PMID: 22679513
  11. discovery of Grb10 as an mTORC1 substrate PMID: 21659604
  12. Grb10 interacts with Bim L and inhibits its proapoptotic activity in a phosphorylation-dependant manner. PMID: 20174874
  13. GRB10 gene is expressed from the paternal allele in the brain, but from the maternal allele in the placental trophoblasts. PMID: 19487367
  14. role in inhibiting insulin-stimulated insulin receptor substrate (IRS)-phosphatidylinositol 3-kinase/Akt signaling pathway by disrupting the association of IRS-1/IRS-2 with the insulin receptor PMID: 12493740
  15. The structure of this protein's SH domains affect ligand specificity. PMID: 12551896
  16. Grb10 acts as a positive regulator in VEGF-R2 signaling and protects VEGF-R2 from degradation by interacting with Nedd4, a component of the endocytic machinery PMID: 15060076
  17. GRB10 protein products are correlated with parameters of birth size. PMID: 15506681
  18. there is a phosphorylation-regulated complex formation of Grb10 with 14-3-3 and Akt PMID: 15722337
  19. Up-regulation of growth factor receptor-bound protein 10 is associated with cervical squamous cell carcinoma PMID: 15870923
  20. Ser(150) and Ser(476) of human Grb10zeta are phosphorylated in intact cells. PMID: 15952796
  21. Together, our results suggest that, in addition to inhibiting IR kinase activity by directly binding to the IR, Grb10 also negatively regulates insulin signaling by mediating insulin-stimulated degradation of the receptor. PMID: 16434550
  22. Single nucleotide polymorphism is associated with type 2 diabetes among Whites. PMID: 16644667
  23. GRB10 is a novel negative regulator of the Wnt signaling pathway. The finding that GRB10 interferes with the binding of Axin to LRP6 indicated a possible molecular mechanism by which the overexpression of GRB10 suppresses Wnt signaling. PMID: 17376403
  24. Binds to the Raf-1 and MEK1 kinases. Potential role in the regulation of apoptosis. PMID: 9553107
  25. Role in the regulation of mitogenesis by receptor tyrosine kinases. PMID: 10454568
  26. Mostly localized to the mitochondria. Can relocalize to the plasma membrane and lamellipodia under certain conditions. PMID: 10585452

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Subcellular Location
Protein Families
GRB7/10/14 family
Tissue Specificity
Widely expressed in fetal and adult tissues, including fetal and postnatal liver, lung, kidney, skeletal muscle, heart, spleen, skin and brain.
Database Links

HGNC: 4564

OMIM: 601523

KEGG: hsa:2887

STRING: 9606.ENSP00000381793

UniGene: Hs.164060

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