HDAC3 Antibody

Code CSB-PA010239LA01HU
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
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  • Western blot
    All lanes: HDAC3 antibody at 2µg/ml + Hela whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 49, 50 kDa
    Observed band size: 49 kDa

  • Western Blot
    Positive WB detected in: Mouse heart tissue
    All lanes: HDAC3 antibody at 2.5µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 49, 50 kDa
    Observed band size: 49 kDa

  • Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA010239LA01HU at dilution of 1:100

  • Chromatin Immunoprecipitation Hela(1.2*106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4μg anti-HDAC3 or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers(CSB-PP010239HU) against the P21 promoter.

  • Immunofluorescent analysis of Hela cells using CSB-PA010239LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) HDAC3 Polyclonal antibody
Uniprot No. O15379
Target Names HDAC3
Alternative Names HD3 antibody; HDAC 3 antibody; HDAC3 antibody; HDAC3_HUMAN antibody; Histone deacetylase 3 antibody; RPD3 2 antibody; RPD3 antibody; RPD3-2 antibody; SMAP45 antibody
Raised in Rabbit
Species Reactivity Human, Mouse
Immunogen Recombinant Human Histone deacetylase 3 protein (1-428AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF, ChIP
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation
Gene References into Functions
  1. The HDAC3 mRNA was expressed more in glioma than in the normal glial cell line. Low HDAC3 mRNA expression levels predicted better overall survival. PMID: 30053564
  2. in rheumatoid arthritis peripheral blood mononuclear cells, the activity and expression of HDAC3 is decreased, which is accompanied with enhanced histone acetyltransferase activity PMID: 30402512
  3. High HDAC3 expression is closely correlated with ER-negative expression, PR-negative expression, HER2 overexpression, PT stage, and clinical stage of breast tumors. PMID: 29680858
  4. these findings indicate that AKAP12 may be a potential prognostic predictor and therapeutic target for the treatment of colorectal cancer in combination with HDAC3 PMID: 29484387
  5. CHD5 was identified as a direct target of miR-454. CHD5 was downregulated in GC tissues/cell lines and the expresssion of CHD5 inversely correlated with the level of miR-454 in GC tissues. Taken together, these observations indicate that HDAC3 is associated with GC cell growth via the miR-454-mediated targeting of CHD5. PMID: 29115379
  6. DANCR associated with EZH2 and HDAC3 to epigenetically silence lncRNA-LET and then regulated gastric cancer cells migration and invasion. PMID: 28951520
  7. Data show there is allosteric communication between the inositol-binding site and the active sites in histone deacetylases HDAC1 and HDAC3. PMID: 27109927
  8. Results show that proteasomal degradation of HDAC1 and HDAC3 by Vpr counteracts HIV-1 latency to reactivate the viral promoter. PMID: 27550312
  9. the NCOR/HDAC3 complex is a major suppressor of differentiation in rhabdomyosarcoma PMID: 27956629
  10. These findings indicate the importance of developing HDAC3-selective inhibitors, and their combined use with osimertinib, for treating EGFR-mutated lung cancers carrying the BIM deletion polymorphism PMID: 27986747
  11. HDAC3-mediated p53 acetylation and oligomerization is induced by apoptosis caused by delphinidin in prostate cancer cells PMID: 27462923
  12. Knockdown of either Xist or SPEN expression in breast cancer cells suppressed the expression of PHLPP1, a phosphatase in AKT dephosphorylation, and was correlated with increased HDAC3 recruitment to the PHLPP1 promoter. PMID: 27248326
  13. Data show that BRCA2 was required for HDAC2/3 association with acetylated BubR1 in nocodazole (Noc)-arrested cells. PMID: 28985013
  14. Data show that the nuclear HDAC3 and cytoplasmic CDH1 have independent prognostic value in pancreatic cancer and provide targets for prognostic therapeutics. PMID: 26918727
  15. HDAC3 uniquely primes Ucp1 and the thermogenic transcriptional program to maintain a critical capacity for thermogenesis in brown adipose tissue that can be rapidly engaged upon exposure to dangerously cold temperature PMID: 28614293
  16. The low expression of HDAC3 and overexpession of inflammatory cytokines (IL-18, IL-12 and TNF-alpha) in intrahepatic cholestasis of pregnancy may be involved in liver cell apoptosis and in the pathophysiology of the disease. PMID: 28697498
  17. SNP rs14251 was found to be significant (and rs2530223 to be nominally significantly associated with the increasing risk of SCZ susceptibility in Han Chinese individuals, suggesting this gene as a potential genetic modifier for SCZ development. PMID: 27573569
  18. Inhibition of HDAC3 with targeted therapy could benefit treatment of the diseases associated with sGCbeta1 down-regulation and/or deficiency such as cancer and several vascular-related diseases. PMID: 27279362
  19. that histone deacetylase 3 interaction with MeCP2 positively regulates a subset of neuronal genes through FOXO deacetylation, and disruption of HDAC3 contributes to cognitive and social impairment PMID: 27428650
  20. miRNA1236 regulates hypoxia-induced epithelial-mesenchymal transformation and metastasis by repressing HDAC3 and SENP1 expression. PMID: 27177472
  21. class I HDACs (HDAC1, 2, 3 and 8) play a major role in regulating extracellular matrix and Epithelial-mesenchymal transition, whereas class IIa HDACs (HDAC4 and 5) are less effective. PMID: 27420561
  22. Histone deacetylase 3 regulates the inflammatory gene expression programme of rheumatoid arthritis fibroblast-like synoviocytes. PMID: 27457515
  23. Study demonstrated an association of elevated HDAC3 activity and HDAC3 mRNA expression in patients with type 2 diabetes (T2DM) which was positively correlated with proinflammation and insulin resistance. PMID: 27904654
  24. HDAC3 upregulation is associated with hepatocellular carcinoma. PMID: 27342975
  25. High HDAC3 expression is associated with pancreatic cancer. PMID: 26745602
  26. These findings pinpoint that TGF-beta represses miR-30d through a Smad2/3-HDAC3-NCoR repression complex and provide novel insights into a potential target for the treatment of podocyte injury-associated glomerulopathies. PMID: 26432290
  27. Results show the direct regulation of CAGE expression by HDAC3 and that HDAC3-CAGE axis regulates the activation of EGFR. HDAC3 targets CAGE to regulate the tumorigenic potential and angiogenic potential of cancer cells. PMID: 26883907
  28. HDAC3 knockdown or HDAC3 inhibition was associated with simultaneous upregulation of the expression of miR130a and downregulation of the expression of TNF1alpha in peripheral blood mononuclear cells. PMID: 26531724
  29. Data suggest that complexes of HDAC3-H1.3 with NCOR1 and NCOR2/SMRT accumulate on chromatin in synchronized HeLa cells in late G2 phase and mitosis; deacetylation activity of HDAC3 is activated via phosphorylation of Ser-424 by CK2 only in mitosis. PMID: 26663086
  30. this is the first report on the regulation mechanism of SIRT7 gene, in which, HDAC3 collaborated with C/EBPalpha to occupy its responding element in the upstream region of SIRT7 gene and repressed its expression in human cells. PMID: 26704017
  31. Among the class II HDACs, HDAC4 interacted with both MR and HDAC3 after aldosterone stimulation. The nuclear translocation of HDAC4 was mediated by protein kinase A (PKA) and protein phosphatases (PP) PMID: 26305553
  32. miR-335 exerted apoptotic effects and inhibited ubiquitination of HDAC3 in anti-cancer drug-resistant cancer cell lines. miR-335 negatively regulated the invasion, migration, and growth rate of cancer cells. PMID: 25997740
  33. Data suggest, in chronic hepatitis C virus infection, HDAC9 (histone deacetylase 9) induction in liver regulates hepatic gluconeogenesis and systemic insulin resistance via deacetylation of FoxO1 (Forkhead box O 1) and HDAC3 (histone deacetylase 3). PMID: 26420860
  34. These data suggest that HDAC3 indirectly modulates tubulin acetylation PMID: 26450925
  35. The inhibition of the transcriptional activity of BCL9-2 by WWOX and HDAC3 constitutes a new molecular mechanism and provides new insight for a broad range of cancers. PMID: 25678599
  36. These observations suggested that HDAC3 plays an important role in the pathological courses of spinal cord injury by regulating miR-130a expression PMID: 25973054
  37. Time-course analysis revealed that HDAC6, HDAC3, and acetylated histone H3 protein levels are significantly inhibited. PMID: 25307283
  38. Aberrant overexpression of HDACs in basal cells of IPF lungs may contribute to the bronchiolisation process in this disease. Similarly, generation and apoptosis resistance of IPF fibroblasts are mediated by enhanced activity of HDAC enzymes. PMID: 26359372
  39. Sequencing of HDAC3 revealed six single-nucleotide polymorphisms. The G allele of rs2530223 was significantly associated with the number of acute medications/month used and with the number of days/month in which medications were used PMID: 26542778
  40. HDAC3 is an essential target to disrupt HIV-1 latency, and inhibition of HDAC2 may also contribute to the effort to purge and eradicate latent HIV-1 infection. PMID: 25136952
  41. HDAC3 contributes to vasculogenic mimicry in gliomas, possibly through the PI3K/ERK-MMPs-laminin5gamma2 signaling pathway. PMID: 25940092
  42. our results uncovered a mechanism by which PINK1-HDAC3 network mediates p53 inhibitory loop in response to oxidative stress-induced damage. PMID: 25305081
  43. SOX4 interacts with EZH2 and HDAC3 to suppress microRNA-31 in invasive esophageal cancer cells. PMID: 25644061
  44. PML-mediated suppression of IL-6-induced STAT3 activation by disrupting interactions between STAT3 and HDAC3. PMID: 25892518
  45. c-Myc contributes to the epigenetic regulation of HPP1 via the dominant recruitment of HDAC3. PMID: 24919179
  46. Data point to HDAC3 as a potential drug target for preserving beta cells against lipotoxicity in diabetes. PMID: 25610877
  47. Htt aggregates impair nuclear proteasome activity through the inhibition of HDAC3 PMID: 25380050
  48. These results demonstrate that mutant H3K27M can be specifically identified with high specificity and sensitivity using an H3K27M antibody and immunohistochemistry to detect high-grade astrocytomas. PMID: 25200321
  49. Findings reveal strong expression of HDAC3 in patients with pancreatic cancer and suggest that HDAC3 participates in the pathogenesis and progression of pancreatic cancer. PMID: 25070540
  50. dysbindin-1 formed a protein complex with HDAC3 in human neuroblastoma cells PMID: 25196196
  51. HDAC3, a new binding regulator of RCAN1, affects the protein stability and intracellular localization of RCAN1. PMID: 25144594
  52. It show the novel role of HDAC3 as a negative regulator of angiogenesis. PMID: 24286308
  53. HDAC3 promotes TIP60 ubiquitination and cytoplasmic localization and protects cells from apoptosis after DNA damage. PMID: 25301942
  54. Data show that Hdac3 physically interacts with Tbx5 and modulates its acetylation to repress Tbx5-dependent activation of cardiomyocyte lineage-specific genes. PMID: 24565863
  55. The nuclear localization signal domain of HDAC3 was necessary, and sufficient for the binding of HDAC3 to the MDR1 promoter sequences PMID: 24286324
  56. selective inhibition of HDAC3 may be an attractive strategy for targeting Type 1 and Type 2 diabetes PMID: 24811789
  57. PP1alpha and class I histone deacetylase (HDAC1/2/3) signaling pathways are essential for the stress-induced BRD4 release from chromatin. PMID: 24939842
  58. MiR-326 as a negative regulator of HDAC3. PMID: 25138213
  59. we further revealed that Stat3 could directly bind to the promoter region of Beclin 1 and repressed its transcription through recruiting histone deacetylase 3 (HDAC3). PMID: 24760274
  60. A significant role for HDAC3 in the MYCN-mediated repression of GRHL1. PMID: 24419085
  61. our study has found no association between HDAC2/HDAC3 gene polymorphisms and schizophrenia in the Chinese Han population PMID: 23857786
  62. model where p53 binding to the SMRT deacetylase activation domain (DAD) limits HDAC3 interaction with this coregulator, thereby facilitating SMRT coactivation of p53-dependent gene expression PMID: 24449765
  63. KDM2A represses histone deacetylase 3 and has a role in tumorigenicity of lung cancer cells PMID: 24482232
  64. study reports a single mutation of HDAC3 that is capable of mimicking the stabilizing effects of deacetylase-activating domain (DAD)and inositol tetraphosphate, without the presence of either. This mutation, however, results in a total loss of deacetylase activity PMID: 23904210
  65. HDAC3 participated in the pathogenesis of children gliomas by promoting glioma cell proliferation and migration PMID: 23685192
  66. HDAC and DNMT protein expression were reduced when methylation-sensitive HCT 116 human colon cancer cells was treated with EGCG, but was relatively stable in the HT-29 cell line PMID: 24324066
  67. results suggest that variants of HDAC3 contribute to an increased prevalence of type 2 diabetes mellitus in the Chinese Han population PMID: 24120624
  68. Data indicate there was a link between baseline viral load, age (40 years), IL-28B (rs12979860), HDAC2 (rs3778216), HDAC3 (rs976552) and HDAC5 (rs368328) with sustained virological response (SVR). PMID: 23615070
  69. HD3alpha directly interacts with HDAC3 and Akt1 and selectively activates transforming growth factor beta2 (TGFbeta2) secretion and cleavage. PMID: 24045946
  70. HDAC3 regulates cyclin A stability. PMID: 23760262
  71. HDAC3 plays a critical role in regulating self-renewal of liver CSCs. PMID: 23879963
  72. HDAC3 is necessary for ERalpha mRNA stability, and is involved in the estrogen-dependent proliferation of ERalpha-positive tumors. PMID: 23402757
  73. Histone deacetylase 3 inhibits expression of PUMA in gastric cancer cells; patients with low PUMA expression have significant decreases in overall survival. PMID: 22763818
  74. Hypoxia-induced histone deacetylase 3 repressed lncRNA-LET by reducing the histone acetylation-mediated modulation of the lncRNA-LET promoter region. PMID: 23395002
  75. Suggest that ethanol affects 5-HT3 and serotonin through mechanisms involving neuronal HDAC1/3. PMID: 22796363
  76. HDAC3 knockdown promotes expansion of CD34+ cells without affecting monocyte lineage commitment. PMID: 22455388
  77. demonstrate that miR-29 is repressed by MYC through a corepressor complex with HDAC3 and EZH2 PMID: 23079660
  78. Data indicate that reversal of histone H3K9/K14 acetylation by histone deacetylase 3 (HDAC3) is required for attenuation of interferon-A (IFN-A) gene transcription during viral infection. PMID: 22685561
  79. PCOS rat models also showed alterations of PPARG1, NCOR1, and HDAC3 mRNA expression and methylation changes of PPARG1 and NCOR1, consistent with the results from humans. PMID: 22349439
  80. In hepatocellular carcinoma cells, up-regulation of HDAC1-3 reduces expression of miR-449, activating hepatocyte growth factor and altering tumor growth. PMID: 22641068
  81. Prox1 recruits the corepressor HDAC3 to directly repress Nkx2.5 via a proximal upstream enhancer as a mechanism for regulating Nkx2.5 function in adult cardiac conduction. PMID: 22647876
  82. The findings highlighted the role of HDAC3 in Myc-induced miR-15a/16-1 changes and reveal novel mechanisms for c-Myc-driven microRNA suppression and malignant transformation in aggressive B-cell malignancies. PMID: 22002311
  83. Chemical inhibition or RNAi knockdown of HDAC3 in human SVG-A cells suppresses DNA trinucleotide repeat expansions. PMID: 22363205
  84. The formation of a stable and active HDAC3-corepressor complex is a stepwise process in which the C terminus of HDAC3 plays a critical role at late steps of the assembly process. PMID: 22337871
  85. the role of HDAC3 and expression of p53 in T cells PMID: 21346816
  86. the structure of a complex between an HDAC and a co-repressor, namely, human HDAC3 with the deacetylase activation domain (DAD) from the human SMRT co-repressor (also known as NCOR2) PMID: 22230954
  87. HDAC3 regulates galectin-9 expression in endothelial cells via interaction with PI3K-IRF3 signal pathway PMID: 22027828
  88. Report HDAC3 expression in histological subtypes of testicular germ cell tumours. PMID: 21972094
  89. HDAC3, not HDAC2, interacts with the major immediate early locus of human cytomegalovirus. PMID: 21453528
  90. HDAC3 and HDAC7 have opposite effects on osteoclast differentiation.( PMID: 21324898
  91. a pathway through which Chk1 inhibition can mediate HDACi-induced mitotic entry and cell death PMID: 21179472
  92. HDAC3 plays a significant role in regulating tumor cell proliferation and invasion, and it could be served as a candidate biomarker for predicting the recurrence of hepatitis B virus-associated hepatocellular carcinoma following liver transplantation PMID: 21206745
  93. combined analysis of HDAC1 SNP rs1741981 and HDAC3 SNP rs2547547 may be a potential genetic marker for hepatocellular carcinoma recurrence in liver transplantation patients. PMID: 20731616
  94. We immunohistochemically analyzed the expression of NCOR1 and 2 as well as HDAC1, 2, and 3 on a tissue microarray comprising tumor samples from 283 astrocytic gliomas PMID: 21143702
  95. RUNX1 interacts with endogenous HDAC1 or HDAC3 PMID: 21059642
  96. HDAC3 is negatively regulated by the nuclear protein DBC1 PMID: 21030595
  97. Strong tumoral expression of HDAC3 is an independent predictor of a poor prognosis in patients with adenocarcinoma of the lung PMID: 20563766
  98. These findings suggest that HDAC3 selectively represses CREB3-mediated transcriptional activation and chemotactic signalling in human metastatic breast cancer cells. PMID: 20473547
  99. results suggested that HDAC1 plays an important role in the proliferation of ovarian cancer cells, whereas HDAC3 functions in cell adhesion and migration PMID: 20049841
  100. The results suggest that HDAC3 genes might play a role in the pathophysiology of schizophrenia in a Korean population. PMID: 20471694

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Subcellular Location Nucleus, Cytoplasm, Cytoplasm, cytosol
Protein Families Histone deacetylase family, HD type 1 subfamily
Tissue Specificity Widely expressed.
Database Links

HGNC: 4854

OMIM: 605166

KEGG: hsa:8841

STRING: 9606.ENSP00000302967

UniGene: Hs.519632

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