HDAC3 Antibody

Datasheet
Code CSB-PA967736
Product Type Polyclonal Antibody
Size US$297
Uniprot No. O15379
Image
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC3 antibody.
  • Immunofluorescence analysis of COS7 cells, using HDAC3 antibody.
  • Western blot analysis of extracts from HepG2 cells, using HDAC3 antibody.
  • Western blot analysis of extracts from HeLa cells (Lane 2), A549 cells (Lane 3) and HepG2 cells (Lane 4), using HDAC3 antiobdy. The lane on the left is treated with systhesized peptide.
Immunogen Synthesized peptide derived from C-terminal of Human HDAC3.
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Specificity The antibody detects endogenous levels of total HDAC3 protein.
Tested Applications ELISA,WB,IHC,IF;WB:1:500-1:3000,IHC:1:50-1:100,IF:1:100-1:500
Relevance Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.

Xiaohong Zhang, Genes & Dev., Apr 2005; 19: 827 - 839.
Zhanguo Gao, J. Biol. Chem., Feb 2006; 281: 4540 - 4547. Andrew J. Wilson, J. Biol. Chem., May 2006; 281: 13548 - 13558.
Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality Polyclonal
Alias Histone deacetylase 3; HD3; RPD3-2; SMAP45;
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Target Names HDAC3
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation
Subcellular Location Nucleus, Cytoplasm, Cytoplasm, cytosol
Protein Families Histone deacetylase family, HD type 1 subfamily
Tissue Specificity Widely expressed.
Database Links

HGNC: 4854

OMIM: 605166

KEGG: hsa:8841

STRING: 9606.ENSP00000302967

UniGene: Hs.519632

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