HIST1H1E (Ab-25) Antibody

^Datasheet

Specification
Target

Code	CSB-PA010380PA25nme1HU
Size	US$319
Uniprot No.	P10412
Image	Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate, MCF-7 whole cell lysate, LO2 whole cell lysate All lanes: HIST1H1E antibody at 1:500 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA010380PA25nme1HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA010380PA25nme1HU at dilution of 1:100 Immunofluorescent analysis of Hela cells using CSB-PA010380PA25nme1HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) Chromatin Immunoprecipitation Hela (4*10⁶) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H1E (CSB-PA010380PA25nme1HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
Protocols	ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol Native Chromatin Immunoprecipitation(ChIP) Protocol
Immunogen	Peptide sequence around site of Lys (25) derived from Human Histone H1.4
Raised in	Rabbit
Species Reactivity	Human
Tested Applications	ELISA, WB, IHC, IF, ChIP; Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200
Relevance	Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Form	Liquid
Conjugate	Non-conjugated
Storage Buffer	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method	Antigen Affinity Purified
Isotype	IgG
Clonality	Polyclonal
Alias	Histone H1.4 (Histone H1b) (Histone H1s-4), HIST1H1E, H1F4
Immunogen Species	Human
Research Area	Epigenetics and Nuclear Signaling
Target Names	HIST1H1E
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Still Have Questions?	Leave a Message or Start an on-line Chat

Function	Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Involvement in disease	Rahman syndrome (RMNS)
Subcellular Location	Nucleus, Chromosome
Protein Families	Histone H1/H5 family
Database Links	HGNC: 4718 OMIM: 142220 KEGG: hsa:3008 STRING: 9606.ENSP00000307705 UniGene: Hs.248133

Related Products

HIST1H1E Antibody

HIST1H1E Protein

HIST1H1E Antibodies

HIST1H1E Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-RA010380A17phHU	Phospho-Histone H1.4 (T17) Antibody	Human	ELISA, IHC, IF

HIST1H1E Antibodies for Human

Code	Product Name	Species Reactivity	Application
CSB-PA010380OA45nacHU	HIST1H1E (Ab-45) Antibody	Human	ELISA, WB, IHC, IF
CSB-PA010380PA45nacHU	HIST1H1E (Ab-45) Antibody	Human	ELISA, IF
CSB-PA010380PA51acHU	Acetyl-HIST1H1E (K51) Antibody	Human	ELISA, ICC, IF, ChIP
CSB-PA010380PA51nacHU	HIST1H1E (Ab-51) Antibody	Human	ELISA, IF
CSB-PA010380PA63acHU	Acetyl-HIST1H1E (K63) Antibody	Human	ELISA, ICC, IF, ChIP
CSB-PA010380PA63nacHU	HIST1H1E (Ab-63) Antibody	Human	ELISA, IHC
CSB-PA010380PA63nme1HU	HIST1H1E (Ab-63) Antibody	Human	ELISA, IHC
CSB-PA010380PA25me1HU	Mono-methyl-HIST1H1E (K25) Antibody	Human	ELISA, ICC, IF
CSB-PA010380PA16me2HU	Di-methyl-HIST1H1E (K16) Antibody	Human	ELISA, ICC, IF, ChIP
CSB-PA010380PA16nme2HU	HIST1H1E (Ab-16) Antibody	Human	ELISA, IF
CSB-PA010380PA03phHU	Phospho-HIST1H1E (T3) Antibody	Human	ELISA, IF
CSB-PA010380PA16acHU	Acetyl-HIST1H1E (K16) Antibody	Human	ELISA, ICC, IF, ChIP
CSB-PA010380PA03nphHU	HIST1H1E (Ab-3) Antibody	Human	ELISA, WB, IHC, IF
CSB-PA010380PA16nacHU	HIST1H1E (Ab-16) Antibody	Human	ELISA, WB, IHC, IF
CSB-PA010380PA26nphHU	HIST1H1E (Ab-26) Antibody	Human	ELISA, WB, IHC, IF
CSB-PA010380PA33acHU	Acetyl-HIST1H1E (K33) Antibody	Human	ELISA, ICC, IF, ChIP
CSB-PA010380PA33nacHU	HIST1H1E (Ab-33) Antibody	Human	ELISA, WB, IHC, IF
CSB-PA010380PA35phHU	Phospho-HIST1H1E (S35) Antibody	Human	ELISA, ICC, IF
CSB-PA010380PA35nphHU	HIST1H1E (Ab-35) Antibody	Human	ELISA, IF
CSB-PA010380PA62nforHU	HIST1H1E (Ab-62) Antibody	Human	ELISA, WB, IHC
CSB-PA010380PA63ncrHU	HIST1H1E (Ab-63) Antibody	Human	ELISA, IHC
CSB-PA010380PA63nforHU	HIST1H1E (Ab-63) Antibody	Human	ELISA, IHC
CSB-PA010380PA147me1HU	Mono-methyl-HIST1H1E (K147) Antibody	Human	ELISA, ICC, IF
CSB-PA010380PA147nme1HU	HIST1H1E (Ab-147) Antibody	Human	ELISA, IF
CSB-PA010380OA145phHU	Phospho-HIST1H1E (T145) Antibody	Human	ELISA, IF
CSB-PA010380OA17phHU	Phospho-HIST1H1E (T17) Antibody	Human	ELISA, WB, IF
CSB-PA010380OA17nphHU	HIST1H1E (Ab-17) Antibody	Human, Rat	ELISA, WB, IHC, IF
CSB-PA010380OA145nphHU	HIST1H1E (Ab-145) Antibody	Human	ELISA, ChIP

HIST1H1E Proteins

HIST1H1E Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP010380HU CSB-EP010380HU CSB-BP010380HU CSB-MP010380HU	Recombinant Human Histone H1.4(HIST1H1E)	Yeast E.coli Baculovirus Mammalian cell

Most popular with customers

2-hydroxyisobutyryl-HIST1H1C (K210) Antibody

Applications: ELISA, ICC

Species Reactivity: Human
2-hydroxyisobutyryl-HIST1H1C (K80) Antibody

Applications: ELISA, ChIP

Species Reactivity: Human
2-hydroxyisobutyryl-HIST1H2BC (K34) Antibody

Applications: ELISA, WB, ICC, IF

Species Reactivity: Human
2-hydroxyisobutyryl-HIST1H4A (K91) Antibody

Applications: ELISA, IF

Species Reactivity: Human
AANAT Antibody

Applications: ELISA, WB, IF

Species Reactivity: Human
ABCA3 Antibody

Applications: ELISA, IHC

Species Reactivity: Human
ABCB7 Antibody

Applications: ELISA, IHC

Species Reactivity: Human
ABCD1 Antibody

Applications: ELISA, IHC

Species Reactivity: Human

Product Center

Kit

Protein

Antibody

Molecular Biology Product

Small Molecule

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Promotions

Products
Service
Technical Resources
Research Tools
About Us
Contact Us
- CUSABIO TECHNOLOGY LLC
- Tel: 301-363-4651
- Email: support@cusabio.com
Join Us with

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

CloseMessage

Product >> Product Name：
Product >> Code：
Product >> Size：
Name： *
Email： *
Phone：
We recommend you to leave your phone number because occasionally our emails get flagged by spam filters. We would not disclose any of your private information to any third party.
Question/Comment： *
Your Region： *
Country State：
I'd like to receive email newsletters from Cusabio

Yes

No
How did you know this promotion?

Email

Distributor

Search engine

Advertisement

Others
Check Code：

HIST1H1E (Ab-25) Antibody

Specification

Target

Related Products

HIST1H1E Antibodies

HIST1H1E Proteins

Most popular with customers

CloseMessage

CloseApplication Form

×CloseMessage