HIST1H1E (Ab-25) Antibody

Datasheet
Code CSB-PA010380PA25nme1HU
Size US$319
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate, MCF-7 whole cell lysate, LO2 whole cell lysate
    All lanes: HIST1H1E antibody at 1:500
    Secondary
    Goat polyclonal to rabbit IgG at 1/40000 dilution
    Predicted band size: 22 kDa
    Observed band size: 22 kDa

  • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA010380PA25nme1HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA010380PA25nme1HU at dilution of 1:100

  • Immunofluorescent analysis of Hela cells using CSB-PA010380PA25nme1HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H1E (CSB-PA010380PA25nme1HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

Product Details

Full Product Name Rabbit anti-Human HIST1H1E Polyclonal antibody
Uniprot No. P10412
Target Names HIST1H1E
Alternative Names Histone H1.4 (Histone H1b) (Histone H1s-4), HIST1H1E, H1F4
Raised in Rabbit
Species Reactivity Human
Immunogen Peptide sequence around site of Lys (25) derived from Human Histone H1.4
Immunogen Species Human
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Antigen Affinity Purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF, ChIP
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:20-1:200
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Target Data

Function Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Involvement in disease Rahman syndrome (RMNS)
Subcellular Location Nucleus, Chromosome
Protein Families Histone H1/H5 family
Database Links

HGNC: 4718

OMIM: 142220

KEGG: hsa:3008

STRING: 9606.ENSP00000307705

UniGene: Hs.248133

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