HIST1H1E (Ab-25) Antibody

Western Blot
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, MCF-7 whole cell lysate, LO2 whole cell lysate
All lanes: HIST1H1E antibody at 1:500
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa

Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA010380PA25nme1HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA010380PA25nme1HU at dilution of 1:100

Immunofluorescent analysis of Hela cells using CSB-PA010380PA25nme1HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Chromatin Immunoprecipitation Hela (4*10⁶) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8μg anti-HIST1H1E (CSB-PA010380PA25nme1HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.