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| Code | CSB-PA010380PA25nme1HU |
| Size | US$319 |
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| Have Questions? | Leave a Message or Start an on-line Chat |
| Full Product Name | Rabbit anti-Homo sapiens (Human) HIST1H1E Polyclonal antibody | ||||||||
| Uniprot No. | P10412 | ||||||||
| Target Names | HIST1H1E | ||||||||
| Alternative Names |
H1 histone family member 4 antibody; H1.4 antibody; H14_HUMAN antibody; H1E antibody; H1F4 antibody; Hist1h1e antibody; Histone 1 H1e antibody; Histone cluster 1 H1e antibody; Histone H1 antibody; Histone H1.4 antibody; Histone H1B antibody; MGC116819 antibody
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| Raised in | Rabbit | ||||||||
| Species Reactivity | Human | ||||||||
| Immunogen | Peptide sequence around site of Lys (25) derived from Human Histone H1.4 | ||||||||
| Immunogen Species | Homo sapiens (Human) | ||||||||
| Conjugate | Non-conjugated | ||||||||
| Clonality | Polyclonal | ||||||||
| Isotype | IgG | ||||||||
| Purification Method | Antigen Affinity Purified | ||||||||
| Concentration | It differs from different batches. Please contact us to confirm it. | ||||||||
| Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
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| Form | Liquid | ||||||||
| Tested Applications | ELISA, WB, IHC, IF, ChIP | ||||||||
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| Protocols | ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol Native Chromatin Immunoprecipitation(ChIP) Protocol |
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| Troubleshooting and FAQs | Antibody FAQs | ||||||||
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | ||||||||
| Lead Time | Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
Applications : Western Blot (WB), Immunohistochemistry (IHC)
Sample type: Human embryonic stem cell lines and HEK293T cells
Sample dilution: IHC: 1:200, WB: 1:1000
Review: IHC Method Human embryonic stem cell line, H7 cells were plated on matrigel pre-coated plate. 3 days after plating cells were fixed with 4% formaldehyde/PBS solution with 4% formaldehyde/PBS for 15 minutes, washed three times with PBS, and then permeabilized with 0.1 % Triton X-100/PBS. Fixed cells were subjected to blocking with 3% BSA/PBS solution and then incubated with primary antibody at 4°C overnight. Primary signals were detected using Alexa Fluor 488 and subsequently DAPI stained. IHC Result IHC result shows strong and clear H1.4 signals in nucleolus. W.B. Method HEKF 293T cells were plated on 6 well plate. 1day later cells were transfected with 1ug of control plasmid (pFUW-vector, addgene #14882) or pFUW-Flag ad Myc tagged-H1.4 plasmid. 2days additional incubation proteins were extracted using GST IP buffer (25 mM Tris-HCl, pH8.0, 150 mM NaCl, 100 mM KCl, 2 mM EDTA, 1% NP-40, and proteinase inhibitor cocktail) for 15 minutes on ice. Total protein was separated on a 12% PAGE gel and 5% skim milk were used for blocking. Primary antibodies were used in blocking solution at 4°C overnight. HRP-conjugated secondary antibodies were used for the detection. W.B. Results CSB-PA010380PA25 recognizes endogenous H1.4 proteins and ectopic overexpressed H1.4 proteins without any non-specific signals.
By Anonymous
| Function |
Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation.
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| Gene References into Functions |
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| Involvement in disease | Rahman syndrome (RMNS) |
| Subcellular Location | Nucleus. Chromosome. Note=Mainly localizes in heterochromatin. Dysplays a punctuate staining pattern in the nucleus. |
| Protein Families | Histone H1/H5 family |
| Database Links |
HGNC: 4718 OMIM: 142220 KEGG: hsa:3008 STRING: 9606.ENSP00000307705 UniGene: Hs.248133 |
