Tel : 301-363-4651
All
  • All
  • Kit
  • Protein
  • Antibody
  • Molecular Biology Product
  • Small Molecule
  • Raw Material For IVD Kit

HIST1H1E (Ab-25) Antibody

Datasheet
Code CSB-PA010380PA25nme1HU
Size US$319
Uniprot No. P10412
Image
  • Western Blotting(WB) - HIST1H1E (Ab-25) Antibody

    Western Blot
    Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate, MCF-7 whole cell lysate, LO2 whole cell lysate
    All lanes: HIST1H1E antibody at 1:500
    Secondary
    Goat polyclonal to rabbit IgG at 1/40000 dilution
    Predicted band size: 22 kDa
    Observed band size: 22 kDa

  • Immunohistochemistry(IHC) 1- HIST1H1E (Ab-25) Antibody

    Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA010380PA25nme1HU at dilution of 1:100

  • Immunohistochemistry(IHC) 2- HIST1H1E (Ab-25) Antibody

    Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA010380PA25nme1HU at dilution of 1:100

  • Immunofluorescence(IF) - HIST1H1E (Ab-25) Antibody

    Immunofluorescent analysis of Hela cells using CSB-PA010380PA25nme1HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Chromatin Immunoprecipitation(ChIP) - HIST1H1E (Ab-25) Antibody

    Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H1E (CSB-PA010380PA25nme1HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

Immunogen Peptide sequence around site of Lys (25) derived from Human Histone H1.4
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IF, ChIP; Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200
Relevance Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method Antigen Affinity Purified
Isotype IgG
Clonality Polyclonal
Alias Histone H1.4 (Histone H1b) (Histone H1s-4), HIST1H1E, H1F4
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Research Area Epigenetics and Nuclear Signaling
Target Names HIST1H1E
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Still Have Questions? Leave a Message or Start an on-line Chat
Function Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Involvement in disease Rahman syndrome (RMNS)
Subcellular Location Nucleus, Chromosome
Protein Families Histone H1/H5 family
Database Links

HGNC: 4718

OMIM: 142220

KEGG: hsa:3008

STRING: 9606.ENSP00000307705

UniGene: Hs.248133

Related Products

HIST1H1E Antibody
HIST1H1E Protein

HIST1H1E Antibodies

HIST1H1E Antibodies for Homo sapiens (Human)

Code Product Name Species Reactivity Application
CSB-RA010380A17phHU Phospho-Histone H1.4 (T17) Antibody Human ELISA, IHC, IF

HIST1H1E Antibodies for Human

Code Product Name Species Reactivity Application
CSB-PA010380OA45nacHU HIST1H1E (Ab-45) Antibody Human ELISA, WB, IHC, IF
CSB-PA010380PA45nacHU HIST1H1E (Ab-45) Antibody Human ELISA, IF
CSB-PA010380PA51acHU Acetyl-HIST1H1E (K51) Antibody Human ELISA, ICC, IF, ChIP
CSB-PA010380PA51nacHU HIST1H1E (Ab-51) Antibody Human ELISA, IF
CSB-PA010380PA63acHU Acetyl-HIST1H1E (K63) Antibody Human ELISA, ICC, IF, ChIP
CSB-PA010380PA63nacHU HIST1H1E (Ab-63) Antibody Human ELISA, IHC
CSB-PA010380PA63nme1HU HIST1H1E (Ab-63) Antibody Human ELISA, IHC
CSB-PA010380PA25me1HU Mono-methyl-HIST1H1E (K25) Antibody Human ELISA, ICC, IF
CSB-PA010380PA16me2HU Di-methyl-HIST1H1E (K16) Antibody Human ELISA, ICC, IF, ChIP
CSB-PA010380PA16nme2HU HIST1H1E (Ab-16) Antibody Human ELISA, IF
CSB-PA010380PA03phHU Phospho-HIST1H1E (T3) Antibody Human ELISA, IF
CSB-PA010380PA16acHU Acetyl-HIST1H1E (K16) Antibody Human ELISA, ICC, IF, ChIP
CSB-PA010380PA03nphHU HIST1H1E (Ab-3) Antibody Human ELISA, WB, IHC, IF
CSB-PA010380PA16nacHU HIST1H1E (Ab-16) Antibody Human ELISA, WB, IHC, IF
CSB-PA010380PA26nphHU HIST1H1E (Ab-26) Antibody Human ELISA, WB, IHC, IF
CSB-PA010380PA33acHU Acetyl-HIST1H1E (K33) Antibody Human ELISA, ICC, IF, ChIP
CSB-PA010380PA33nacHU HIST1H1E (Ab-33) Antibody Human ELISA, WB, IHC, IF
CSB-PA010380PA35phHU Phospho-HIST1H1E (S35) Antibody Human ELISA, ICC, IF
CSB-PA010380PA35nphHU HIST1H1E (Ab-35) Antibody Human ELISA, IF
CSB-PA010380PA62nforHU HIST1H1E (Ab-62) Antibody Human ELISA, WB, IHC
CSB-PA010380PA63ncrHU HIST1H1E (Ab-63) Antibody Human ELISA, IHC
CSB-PA010380PA63nforHU HIST1H1E (Ab-63) Antibody Human ELISA, IHC
CSB-PA010380PA147me1HU Mono-methyl-HIST1H1E (K147) Antibody Human ELISA, ICC, IF
CSB-PA010380PA147nme1HU HIST1H1E (Ab-147) Antibody Human ELISA, IF
CSB-PA010380OA145phHU Phospho-HIST1H1E (T145) Antibody Human ELISA, IF
CSB-PA010380OA17phHU Phospho-HIST1H1E (T17) Antibody Human ELISA, WB, IF
CSB-PA010380OA17nphHU HIST1H1E (Ab-17) Antibody Human, Rat ELISA, WB, IHC, IF
CSB-PA010380OA145nphHU HIST1H1E (Ab-145) Antibody Human ELISA, ChIP

HIST1H1E Proteins

HIST1H1E Proteins for Homo sapiens (Human)

Code Product Name Source
CSB-YP010380HU
CSB-EP010380HU
CSB-BP010380HU
CSB-MP010380HU
 Recombinant Human Histone H1.4(HIST1H1E) Yeast
E.coli
Baculovirus
Mammalian cell

Most popular with customers

Product Center

Kit

Protein

Antibody

Molecular Biology Product

Small Molecule

Related Articles
Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?
Promotions

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.

Wait!

Join the 25,000 subscribers to get research hotpots, technical tips, latest information on events, sales and offers.

Sign up now!

We don't deal in spam.