HIST1H1E (Ab-25) Antibody

Code CSB-PA010380PA25nme1HU
Size US$319
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate, MCF-7 whole cell lysate, LO2 whole cell lysate
    All lanes: HIST1H1E antibody at 1:500
    Goat polyclonal to rabbit IgG at 1/40000 dilution
    Predicted band size: 22 kDa
    Observed band size: 22 kDa

  • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA010380PA25nme1HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA010380PA25nme1HU at dilution of 1:100

  • Immunofluorescent analysis of Hela cells using CSB-PA010380PA25nme1HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H1E (CSB-PA010380PA25nme1HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

Have Questions? Leave a Message or Start an on-line Chat

Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) HIST1H1E Polyclonal antibody
Uniprot No. P10412
Target Names HIST1H1E
Alternative Names
H1 histone family member 4 antibody; H1.4 antibody; H14_HUMAN antibody; H1E antibody; H1F4 antibody; Hist1h1e antibody; Histone 1 H1e antibody; Histone cluster 1 H1e antibody; Histone H1 antibody; Histone H1.4 antibody; Histone H1B antibody; MGC116819 antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Peptide sequence around site of Lys (25) derived from Human Histone H1.4
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Antigen Affinity Purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF, ChIP
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:20-1:200
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 1 reviews

Submit a Review here

Applications : Western Blot (WB), Immunohistochemistry (IHC)

Sample type: Human embryonic stem cell lines and HEK293T cells

Sample dilution: IHC: 1:200, WB: 1:1000

Review: IHC Method Human embryonic stem cell line, H7 cells were plated on matrigel pre-coated plate. 3 days after plating cells were fixed with 4% formaldehyde/PBS solution with 4% formaldehyde/PBS for 15 minutes, washed three times with PBS, and then permeabilized with 0.1 % Triton X-100/PBS. Fixed cells were subjected to blocking with 3% BSA/PBS solution and then incubated with primary antibody at 4°C overnight. Primary signals were detected using Alexa Fluor 488 and subsequently DAPI stained. IHC Result IHC result shows strong and clear H1.4 signals in nucleolus. W.B. Method HEKF 293T cells were plated on 6 well plate. 1day later cells were transfected with 1ug of control plasmid (pFUW-vector, addgene #14882) or pFUW-Flag ad Myc tagged-H1.4 plasmid. 2days additional incubation proteins were extracted using GST IP buffer (25 mM Tris-HCl, pH8.0, 150 mM NaCl, 100 mM KCl, 2 mM EDTA, 1% NP-40, and proteinase inhibitor cocktail) for 15 minutes on ice. Total protein was separated on a 12% PAGE gel and 5% skim milk were used for blocking. Primary antibodies were used in blocking solution at 4°C overnight. HRP-conjugated secondary antibodies were used for the detection. W.B. Results CSB-PA010380PA25 recognizes endogenous H1.4 proteins and ectopic overexpressed H1.4 proteins without any non-specific signals.

By Anonymous

Target Background

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation.
Gene References into Functions
  1. Results show that histones H1.2 and H1.4 were observed in MDA-MB-231 metastatic breast cancer cells. The phosphorylation at S173 of histone H1.2 and S172, S187, T18, T146, and T154 of H1.4 significantly increases during M phase suggesting that these events are cell cycle-dependent. PMID: 26209608
  2. This study identified and confirmed HIST1H1E protein changes within the postsynaptic density in schizophrenia. PMID: 25048004
  3. the N-terminal domain of H1 is an important determinant of affinity and specificity of H1-chromatin interactions. PMID: 22425985
  4. PKA-mediated H1.4S35 phosphorylation dissociates H1.4 from mitotic chromatin but also suggest that this phosphorylation is necessary for specific mitotic functions. PMID: 21852232
  5. Allele-specific underacetylation of histone H4 downstream from promoter is associated with X-inactivation in human cells. PMID: 12498347
  6. the lysine residue adjacent to the phosphorylation site found on the serine residue on the H1.4 peptide KARKSAGAAKR was also shown to be methylated, raising the question of whether the hypothesized "methyl/phos" switch could be extended to linker histones PMID: 15595731
  7. Dynamic Histone H1 Isotype 4 Methylation and Demethylation by Histone Lysine Methyltransferase G9a/KMT1C and the Jumonji Domain-containing JMJD2/KDM4 Proteins PMID: 19144645

Show More

Hide All

Involvement in disease Rahman syndrome (RMNS)
Subcellular Location Nucleus. Chromosome. Note=Mainly localizes in heterochromatin. Dysplays a punctuate staining pattern in the nucleus.
Protein Families Histone H1/H5 family
Database Links

HGNC: 4718

OMIM: 142220

KEGG: hsa:3008

STRING: 9606.ENSP00000307705

UniGene: Hs.248133

Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
7505 Fannin St Ste 610-322 Houston, TX 77054, USA
Join Us with

Subscribe newsletter

Leave a message

© 2007-2023 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1