Recombinant Enterococcus faecium Vancomycin/teicoplanin A-type resistance protein VanA (vanA)

Code CSB-YP333043ELX
MSDS
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Source Yeast
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Code CSB-EP333043ELX
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Source E.coli
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Code CSB-EP333043ELX-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP333043ELX
MSDS
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Source Baculovirus
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Code CSB-MP333043ELX
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
vanA
Uniprot No.
Alternative Names
vanA; Vancomycin/teicoplanin A-type resistance protein VanA; EC 6.1.2.1; D-alanine--D-lactate ligase; VanA ligase
Species
Enterococcus faecium (Streptococcus faecium)
Expression Region
1-343
Target Protein Sequence
MNRIKVAILF GGCSEEHDVS VKSAIEIAAN INKEKYEPLY IGITKSGVWK MCEKPCAEWE NDNCYSAVLS PDKKMHGLLV KKNHEYEINH VDVAFSALHG KSGEDGSIQG LFELSGIPFV GCDIQSSAIC MDKSLTYIVA KNAGIATPAF WVINKDDRPV AATFTYPVFV KPARSGSSFG VKKVNSADEL DYAIESARQY DSKILIEQAV SGCEVGCAVL GNSAALVVGE VDQIRLQYGI FRIHQEVEPE KGSENAVITV PADLSAEERG RIQETAKKIY KALGCRGLAR VDMFLQDNGR IVLNEVNTLP GFTSYSRYPR MMAAAGIALP ELIDRLIVLA LKG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for high-level resistance to glycopeptide antibiotics. D-Ala--D-Ala ligase of altered specificity which catalyzes ester bond formation between D-Ala and various D-hydroxy acids; produces a peptidoglycan which does not terminate in D-alanine but in D-lactate, thus preventing vancomycin or teicoplanin binding.
Gene References into Functions
  1. Vancomycin-resistant Enterococcus faecium and Enterococcus faecalis in the West Balkans carry plasmids expressing vanA and vanB genes. PMID: 27351410
  2. Enterococcus faecium strains carrying both vanB and vanA are identified in hospitalized glycopeptide-resistant patients. PMID: 24092659
Subcellular Location
Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families
D-alanine--D-alanine ligase family
Database Links

KEGG: ag:CAA40215

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