Recombinant Human Calpain-1 catalytic subunit (CAPN1)

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Code CSB-EP004490HUc7
MSDS
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 95% as determined by SDS-PAGE.
Activity
Not Test
Target Names
Uniprot No.
Research Area
Cell Biology
Alternative Names
(Calcium-activated neutral proteinase 1)(CANP 1)(Calpain mu-type)(Calpain-1 large subunit)(Cell proliferation-inducing gene 30 protein)(Micromolar-calpain)(muCANP)
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
1-714aa
Target Protein Sequence
MSEEIITPVYCTGVSAQVQKQRARELGLGRHENAIKYLGQDYEQLRVRCLQSGTLFRDEAFPPVPQSLGYKDLGPNSSKTYGIKWKRPTELLSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNDTLLHRVVPHGQSFQNGYAGIFHFQLWQFGEWVDVVVDDLLPIKDGKLVFVHSAEGNEFWSALLEKAYAKVNGSYEALSGGSTSEGFEDFTGGVTEWYELRKAPSDLYQIILKALERGSLLGCSIDISSVLDMEAITFKKLVKGHAYSVTGAKQVNYRGQVVSLIRMRNPWGEVEWTGAWSDSSSEWNNVDPYERDQLRVKMEDGEFWMSFRDFMREFTRLEICNLTPDALKSRTIRKWNTTLYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLDETDDPDDYGDRESGCSFVLALMQKHRRRERRFGRDMETIGFAVYEVPPELVGQPAVHLKRDFFLANASRARSEQFINLREVSTRFRLPPGEYVVVPSTFEPNKEGDFVLRFFSEKSAGTVELDDQIQANLPDEQVLSEEEIDENFKALFRQLAGEDMEISVKELRTILNRIISKHKDLRTKGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLSIFRKFDLDKSGSMSAYEMRMAIESAGFKLNKKLYELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKTLDTDLDGVVTFDLFKWLQLTMFA
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
88.8
Protein Length
Full Length
Tag Info
C-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409'.
Gene References into Functions
  1. Unexpected role of the L-domain of calpastatin during the autoproteolytic activation of human erythrocyte calpain. PMID: 29572388
  2. Studies indicate that reactive oxygen and nitrogen species (RONS) and calpain play important roles in the development of airway and pulmonary vascular remodeling in COPD. PMID: 29047085
  3. Calpain-1 was significantly expressed in Triple-negative breast cancer tissues varying from low to high with a significant correlation to lymph node status but not with the other clinicopathological variables, suggesting its role as a prognostic factor PMID: 28536704
  4. These findings suggest that calpain and AR-V7 may serve as important biomarkers in the treatment of castration-resistant prostate cancer , and targeting calpain and AR-V7 may provide a new approach in overcoming docetaxel-resistance. PMID: 28498452
  5. Rare homozygous and compound-heterozygous nonsense, missense, frameshift, and splice-site mutations in CAPN1 were identified in all hereditary spastic paraplegia affected individuals, and sequencing in additional family members confirmed the segregation of these mutations with the disease. PMID: 27153400
  6. the cleavage of myoferlin, yielding a membrane-associated dual C2 domain 'mini-myoferlin', is reported. PMID: 28192161
  7. mutations in CAPN1 are an additional cause of ataxia in mammals, including humans. PMID: 27320912
  8. Calpain-1 expression is associated with poor relapse-free survival in breast cancer patients treated with trastuzumab. PMID: 26496999
  9. Regulation of platelet-activating factor-mediated PTP1B activation by a Janus kinase 2/ calpain pathway has been reported. PMID: 28686728
  10. HRNR deimination improves its cross-linking by TGases and its proteolytic processing by calpain-1. PMID: 27742573
  11. calpains and calpastatin in patients with idiopathic pulmonary arterial hypertension (PAH) and mice with hypoxic or spontaneous (SM22-5HTT(+) strain) PH, were investigated. PMID: 26974350
  12. calpain and protein kinase Calpha abnormal release promotes a constitutive release of matrix metalloproteinase 9 in peripheral blood mononuclear cells from cystic fibrosis patients PMID: 27349634
  13. Endoplasmic reticulum stress may be associated with apoptosis of LECs, resulting in cataract formation in diabetic patients. PMID: 27130368
  14. our findings suggest that the gene/protein expression of both CAPN1 and CAPN2, as well as the ERK1 and ERK2 genes and related proteins, could be molecular factors associated with more invasive tumor behavior in squamous cell laryngeal cancer. PMID: 27456359
  15. This study demonstrated that Upregulation of calpain activity precedes tau phosphorylation and loss of synaptic proteins in Alzheimer's disease brain. PMID: 27036949
  16. the calpain-dependent cleavage of Nav1.6 channels expressed in human embryonic kidney (HEK) 293 cells caused the upregulation of I(NaP) PMID: 26974309
  17. Increased mu-Calpain Activity in Blasts of Common B-Precursor Childhood Acute Lymphoblastic Leukemia Correlates with Their Lower Susceptibility to Apoptosis. PMID: 26317226
  18. Data indicate that calpain is responsible for processing translation termination factor eRF3 into its IAP-binding isoform p-eRF3. PMID: 26172506
  19. NOS stimulation via PI3K, calpain proteases, and SIRT1-dependent deacetylation downstream from VEGFR2 activation contributes to these vasodilator responses. PMID: 26284543
  20. Junctophilin-2 is cleaved by calpain at multiple sites, resulting in dysfunctional junctophilin-2 truncations. PMID: 26063807
  21. The results of this study indicated that reductions in kallikrein-6 and calpain-1 may contribute to the accumulation of alpha-synuclein in DLB. PMID: 25476568
  22. findings suggest that truncation/activation of Dyrk1A by Ca(2+)/calpain I might contribute to Tau pathology via promotion of exon 10 exclusion and hyperphosphorylation of Tau in AD brain PMID: 25918155
  23. 5-LOX inhibition reduced apoptotic death, restored the initial IL-2/INF-gamma ratio, and more importantly reverted micro-calpain activation induced by simulated microgravity. PMID: 25309925
  24. the p12/Pol delta is a target as a nuclear substrate of mu-calpain in a calcium-triggered apoptosis and appears to be a potential marker in the study of the chemotherapy of cancer therapies. PMID: 24691096
  25. Data show the X-ray structures of calpain with (Z)-3-(4-chlorophenyl)-2-mercaptoacrylic acid and (Z)-3-(5-bromoindol-3-yl)-2-mercaptoacrylic acid. PMID: 25086406
  26. CAPN1 anchors to two NCX1 regions and cleaves at methionine-369, leading to NCX1 inactivation in heart failure. PMID: 25336645
  27. Besides, calpain-1, a calcium-dependent cysteine protease, was involved inherpes simplex virus type 1 intracellular trafficking. PMID: 24670325
  28. Ayloid beta mediates calpain cleavage of NCX3 in Alzheimer's disease brain. PMID: 23919677
  29. Increased trophoblast apoptosis and altered expression levels of syncytin-1, calpain 1, and AIF is observed in preeclamptic placentas. PMID: 24413738
  30. The PC1 function on JAK2 and ERK signaling pathways might be regulated by calpains in response to the changes in intracellular calcium concentration. PMID: 24416790
  31. Small interfering RNA (siRNA)-mediated silencing of micro-calpain expression significantly suppressed the adhesive, migrative and invasive potentials of human hepatoma cells. PMID: 23733271
  32. HER2 inhibited calpain-1 activity through upregulating calpastatin, an endogenous calpain inhibitor. PMID: 23707532
  33. Calpain activation via Ca(2) flux plays an essential role in eliciting an AIF-mediated, caspase-independent apoptotic pathway in EV71-infected cells. PMID: 23515028
  34. a truncated fragment of Src protein kinase generated by calpain-mediated cleavage is a mediator of neuronal death in excitotoxicity PMID: 23400779
  35. Extracellular signal-regulated kinase and glycogen synthase kinase 3beta regulate gephyrin postsynaptic aggregation and GABAergic synaptic function in a calpain-dependent mechanism PMID: 23408424
  36. Proteolysis of calcineurin A by endogenous calpain I leads to the formation of constitutively active calcineurin in the human heart, which may contribute to the pathogenesis of myocardial disease PMID: 23289183
  37. Data indicate that calpain-1 plays a pivotal role in matrix metalloproteinase 2 (MMP2) activation and extracellular matrix remodeling, including fibrosis and vascular calcification. PMID: 23006733
  38. Report synthesis of emodin derivatives, emodin carbaldehyde, citreorosein, and their 10-deoxygenated derivatives and their inhibitory activities on mu-calpain. PMID: 22477191
  39. Results show that estrogen may contribute to both up-regulation and proteolysis of cyclin E through calpain in MCF-7 cells. PMID: 22449977
  40. APP secretion from the calpain overexpressing cells was robustly increased under both resting and stimulated conditions over wild-type cells. PMID: 22480599
  41. Overexpression of mu-calpain in the anterior temporal neocortex is associated with intractable epilepsy as well as the clinicopathological characteristics in these patients. PMID: 21315622
  42. Data show neutrophil calpain I (mu-calpain) was inactiveted by alpha1-antitrypsin. PMID: 21494752
  43. With higher grading of the ovarian carcinoma, staining intensity and immunoreactive score of calpain 1 decreased. PMID: 22335024
  44. Results show high levels of the 17-kDa tau fragment and enhanced calpain activity in the temporal cortex of AD patients and in brain samples obtained from patients with other tauopathies. PMID: 21442128
  45. The expression of mu-calpain-induced degradation products of extracellular matrix was correlated with the degree of disc degeneration in human intervertebral disc tissue PMID: 21839844
  46. siRNA knockdown of calpain prevented deltamethrin-induced DNA fragmentation. PMID: 21555338
  47. it is possible to protect cells from paclitaxel-induced degradation of NCS-1 by inhibiting calpain activity PMID: 21808066
  48. Data show that transfection of cells with GRP78 or calpain I and calpain II siRNA reduced MPTB-mediated cell apoptosis in chondrosarcoma JJ012 cells PMID: 21374734
  49. Findings show that p73alpha and its regulation by the Ca(2+)-mediated calpain pathway are involved in CDDP-induced apoptosis in OVCA cells and that dysregulation of Ca(2+)/calpain/p73 signaling may in part be the pathophysiology of CDDP resistance. PMID: 21516125
  50. Rad21 cleavage by calpain-1 promotes separation of chromosome arms, which coincides with a calcium-induced partial loss of cohesin at several chromosomal loci. PMID: 21876002

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Involvement in disease
Spastic paraplegia 76, autosomal recessive (SPG76)
Subcellular Location
Cytoplasm. Cell membrane.
Protein Families
Peptidase C2 family
Tissue Specificity
Ubiquitous.
Database Links

HGNC: 1476

OMIM: 114220

KEGG: hsa:823

STRING: 9606.ENSP00000279247

UniGene: Hs.502842

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