Recombinant Human E3 SUMO-protein ligase EGR2(EGR2)

Code CSB-YP007485HU
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Source Yeast
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Code CSB-EP007485HU
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Source E.coli
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Code CSB-EP007485HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007485HU
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Source Baculovirus
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Code CSB-MP007485HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names EGR2
Uniprot No. P11161
Alternative Names AT591; CMT1D; CMT4E; DKFZp686J1957; E3 SUMO-protein ligase EGR2; Early growth response 2; Early growth response protein 2; EGR-2; egr2; EGR2_HUMAN; FLJ14547; KROX 20 Drosophila homolog; Krox 20 homolog Drosophila; KROX-20; Drosophila; homolog (early growth response-2); KROX20; Krox20 protein; Zinc finger protein Krox-20
Species Homo sapiens (Human)
Expression Region 1-476
Target Protein Sequence MMTAKAVDKI PVTLSGFVHQ LSDNIYPVED LAATSVTIFP NAELGGPFDQ MNGVAGDGMI NIDMTGEKRS LDLPYPSSFA PVSAPRNQTF TYMGKFSIDP QYPGASCYPE GIINIVSAGI LQGVTSPAST TASSSVTSAS PNPLATGPLG VCTMSQTQPD LDHLYSPPPP PPPYSGCAGD LYQDPSAFLS AATTSTSSSL AYPPPPSYPS PKPATDPGLF PMIPDYPGFF PSQCQRDLHG TAGPDRKPFP CPLDTLRVPP PLTPLSTIRN FTLGGPSAGV TGPGASGGSE GPRLPGSSSA AAAAAAAAAY NPHHLPLRPI LRPRKYPNRP SKTPVHERPY PCPAEGCDRR FSRSDELTRH IRIHTGHKPF QCRICMRNFS RSDHLTTHIR THTGEKPFAC DYCGRKFARS DERKRHTKIH LRQKERKSSA PSASVPAPST ASCSGGVQPG GTLCSSNSSS LGGGPLAPCS SRTRTP
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Sequence-specific DNA-binding transcription factor. Plays a role in hindbrain segmentation by regulating the expression of a subset of homeobox containing genes and in Schwann cell myelination by regulating the expression of genes involved in the formation and maintenance of myelin. Binds to two EGR2-consensus sites EGR2A (5'-CTGTAGGAG-3') and EGR2B (5'-ATGTAGGTG-3') in the HOXB3 enhancer and promotes HOXB3 transcriptional activation. Binds to specific DNA sites located in the promoter region of HOXA4, HOXB2 and ERBB2. Regulates hindbrain segmentation by controlling the expression of Hox genes, such as HOXA4, HOXB3 and HOXB2, and thereby specifying odd and even rhombomeres. Promotes the expression of HOXB3 in the rhombomere r5 in the hindbrain. Regulates myelination in the peripheral nervous system after birth, possibly by regulating the expression of myelin proteins, such as MPZ, and by promoting the differentiation of Schwann cells. Involved in the development of the jaw openener musculature, probably by playing a role in its innervation through trigeminal motor neurons. May play a role in adipogenesis, possibly by regulating the expression of CEBPB.; E3 SUMO-protein ligase helping SUMO1 conjugation to its coregulators NAB1 and NAB2, whose sumoylation down-regulates EGR2 transcriptional activity.
Gene References into Functions
  1. Low EGR2 expression is associated with hepatocellular carcinoma. PMID: 28542387
  2. The study suggests that acetylation of EGR2 is regulated independently of nucleosome remodeling and deacetylase. PMID: 28576496
  3. Egr2-driven cell surface proteins LAG-3 and 4-1BB can identify dysfunctional tumor antigen-specific CD8(+) TIL. PMID: 28115575
  4. Egr2 and Egr3 have emerged as regulatory molecules that suppress excessive immune responses. Mice deficient for Egr2 and Egr3 develop a lupus-like disease with dysregulated activation of effector T cells. Egr2 and Egr3 confer suppressive activity to CD4(+) T cells and regulate the production of inhibitory cytokines such as IL-10 and TGF-beta1. PMID: 27856665
  5. Analysis of consensus EGR-binding elements (EBEs) showed that the immediate early response 3 gene (IER3) is a novel transcriptional target gene of EGR2 as confirmed by the luciferase assay, electrophoretic mobility-shift assay (EMSA), chromatin immunoprecipitation (ChIP), and western blot analysis. PMID: 27890615
  6. In the PPI network, genes may be involved in Down syndrome (DS) by interacting with others, including nuclear receptor subfamily 4 group A member 2 (NR4A2)early growth response (EGR)2 and NR4A2EGR3. Therefore, RUNX1, NR4A2, EGR2, EGR3 and ID4 may be key genes associated with the pathogenesis of DS. PMID: 27667480
  7. EGR2 mutation presents as an axonal Charcot-Marie-Tooth phenotype with variable severity. PMID: 26204789
  8. MicroRNA20a promotes the proliferation and cell cycle of human osteosarcoma cells by suppressing EGR2 expression. PMID: 26238942
  9. These results suggested that EGR2 overexpression has a pivotal role in the downregulation of cytokines implicated in the pathophysiology of Guillain-Barre syndrome PMID: 26718337
  10. A recurrent mutation was identified in EGR2 which appears to be associated with the pathogenesis of schizophrenia. PMID: 26119399
  11. Knock-down of EGR2 with siRNA was demonstrated to have a similar effect as the over-expression of miR-330-3p in NSCLC cell lines PMID: 25935837
  12. Dysregulated Egr-2 is observed in some human autoimmune disorders. PMID: 25381473
  13. EGR2 knockdown inhibited proliferation, clonogenicity and spheroidal growth in vitro and induced regression of Ewing sarcoma xenografts. PMID: 26214589
  14. transcription factor early growth response gene-2 is a novel molecular switch regulating known immunomodulatory molecules in human mesenchymal stem cells. PMID: 24007274
  15. Overexpression of EGR2 significantly attenuated the oncogenic effect of miR-20a. PMID: 23924943
  16. Lack of association of EGR2 variants with bipolar disorder in Japanese population. PMID: 23747400
  17. EGR2 and EGR3 are regulated by NFkappaB and MAPK signalling pathways downstream of TNFalpha stimulation in breast adipose fibroblasts, and this in turn is upstream of CYP19A1 transcription via PI.4 PMID: 23485457
  18. data suggest a tendency of gender-specific association of EGR2 and EGR4 in schizophrenia. PMID: 22691714
  19. Charcot-Marie-Tooth neuropathy due to a novel EGR2 gene mutation exhibits a mild phenotype in a Czech family. PMID: 22546699
  20. Egr2 expression is translationally regulated via an IRES element, which is responsive to an inflammatory environment. PMID: 22915601
  21. we report on a heterozygous mutation in EGR2 in a family with a mild demyelinating form of adult-onset Charcot-Marie-Tooth disease PMID: 22734907
  22. Combined with a plausible biological function of EGR2, the EGR2 gene is a possible susceptibility gene in bipolar disorder. PMID: 22089088
  23. data further demonstrate the major role of the myelinating Schwann cell element in the regulation of EGR2 expression in the human peripheral nervous system PMID: 22522483
  24. Results identify a mutation in EGR2 in a Charcot-Marie-tooth disease patient with hypersensitivity to vincristine. PMID: 22271166
  25. Common variants near TARDBP and EGR2 are associated with susceptibility to Ewing sarcoma. PMID: 22327514
  26. Krox20 functions as a SUMO ligase for its coregulators--the Nab proteins--and that Nab sumoylation negatively modulates Krox20 transcriptional activity in vivo. PMID: 21836637
  27. TGF-beta3 regulated Egr-2 gene expression in uterine leiomyoma cells. PMID: 21703609
  28. these findings suggest that Egr-2 plays an important nonredundant role in the pathogenesis of fibrosis. PMID: 21514423
  29. EGR2 is a direct transcriptional target of p53 family that can in part mediate the p53-dependent apoptotic pathway. PMID: 21042708
  30. Schwann cells expressed myelin proteins and Krox20 which is an important regulator of peripheral myelination. PMID: 21057508
  31. EGR2 is a genetic risk factor for Systemic lupus erythematosus (SLE), in which increased gene expression may contribute to SLE pathogenesis. PMID: 20194224
  32. EGR2 mutation(amino aacid substitution) identifies a case of Charcot-Marie-Totth disease. PMID: 20513111
  33. overexpression of miR-150 in gastric cancer could promote proliferation and growth of cancer cells at least partially through directly targeting the tumor-suppressor EGR2. PMID: 20067763
  34. Frequency of mutations in the early growth response 2 gene associated with peripheral demyelinating neuropathies PMID: 12471219
  35. EGR2 mutant D355V induces a Cx32 promoter, mutant R381H doesn't. A sequence located at -216, recognized by the wild-type and the mutant D355V recombinant proteins, is relevant for promoter transactivation. PMID: 12609493
  36. we found that EGR2 could induce apoptosis in a large proportion of these lines by altering the permeability of mitochondrial membranes, releasing cytochrome c and activating caspase-3, -8, and -9. PMID: 12687019
  37. This report detected a heterozygous Asp383Tyr mutation of EGR2 in one patient with severe CMT1, Dejerine-Sottas syndrome. PMID: 12736090
  38. a possible molecular mechanism to account for down-regulation of EGR2 in tumor cells PMID: 14596916
  39. Egr-2 and Egr-3 transcription is enhanced by Hepatitis B virus X protein, which induces fasL gene expression PMID: 15173177
  40. A Charcot-Marie-Tooth syndrome in a family with a missense mutation in EGR2. PMID: 15947997
  41. One novel de-novo mutation (Arg359Gln, R359Q) was identified in heterozygous state in a patient with a typical CMT1 phenotype, progressive moderate thoracolumbar scoliosis and without clinical signs of cranial nerve dysfunction. PMID: 16198564
  42. Control of Krox20 transcription relies on three very long-range enhancer elements (A, B and C) that are conserved in chick, mouse and human genomes. PMID: 16495311
  43. morphological studies in the context of the I268N homozygous recessive mutation affecting the NAB repressor binding site and the R359W dominant-negative mutation in the zinc-finger domain PMID: 17717711
  44. Collectively, these data indicate that EGR2 is specifically upregulated by a parasite-derived secreted factor that is most likely a resident rhoptry protein. PMID: 18678671
  45. Dysregulation of EGR2 and MeCP2 plays roles in in Rett syndrome and autism. PMID: 19000991
  46. AIP2 regulates activation-induced T-cell death by suppressing EGR2-mediated FasL expression via the ubiquitin pathway PMID: 19651900

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Involvement in disease Neuropathy, congenital hypomyelinating or amyelinating (CHN); Charcot-Marie-Tooth disease 1D (CMT1D); Dejerine-Sottas syndrome (DSS)
Subcellular Location Nucleus.
Protein Families EGR C2H2-type zinc-finger protein family
Database Links

HGNC: 3239

OMIM: 129010

KEGG: hsa:1959

STRING: 9606.ENSP00000242480

UniGene: Hs.1395

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