Product Details

Uniprot No.

Target Names

Alternative Names

E3 SUMO-protein ligase EGR2 (EC 2.3.2.-) (AT591) (E3 SUMO-protein transferase ERG2) (Early growth response protein 2) (EGR-2) (Zinc finger protein Krox-20), EGR2, KROX20

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from Human EGR2

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

27C3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

EGR2, also known as Krox-20, is a zinc finger transcription factor that plays essential roles in peripheral nerve myelination, hindbrain development, and immune cell differentiation. As a key regulator of Schwann cell development and myelin gene expression, EGR2 has become an important target for researchers investigating peripheral neuropathies, neurodevelopmental processes, and the molecular mechanisms underlying myelination disorders. Beyond neuroscience, EGR2 functions as an E3 SUMO-protein ligase and participates in epigenetic regulatory networks, making it relevant to studies of transcriptional control and cellular differentiation.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human EGR2, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and produced recombinantly, researchers can expect minimal lot-to-lot variation, ensuring reliable results across extended studies and collaborative projects.

Validation data demonstrates robust performance across multiple applications. In western blot experiments, the antibody detects a band at the predicted molecular weight of 50 kDa across diverse sample types, including human neuroblastoma cells (SH-SY5Y), glioblastoma cells (U251), breast cancer cells (MCF-7), leukemia cells (K562), and mouse brain tissue lysates. This cross-species reactivity between human and mouse samples provides flexibility for researchers working with different model systems. Flow cytometry validation in SH-SY5Y cells confirms utility for intracellular protein detection, with clear signal separation from isotype controls.

Whether investigating Schwann cell biology, characterizing transcription factor networks, or exploring EGR2's role in neurological disease models, this antibody provides a dependable tool for epigenetics, nuclear signaling, and neuroscience research applications.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Sequence-specific DNA-binding transcription factor. Plays a role in hindbrain segmentation by regulating the expression of a subset of homeobox containing genes and in Schwann cell myelination by regulating the expression of genes involved in the formation and maintenance of myelin. Binds to two EGR2-consensus sites EGR2A (5'-CTGTAGGAG-3') and EGR2B (5'-ATGTAGGTG-3') in the HOXB3 enhancer and promotes HOXB3 transcriptional activation. Binds to specific DNA sites located in the promoter region of HOXA4, HOXB2 and ERBB2. Regulates hindbrain segmentation by controlling the expression of Hox genes, such as HOXA4, HOXB3 and HOXB2, and thereby specifying odd and even rhombomeres. Promotes the expression of HOXB3 in the rhombomere r5 in the hindbrain. Regulates myelination in the peripheral nervous system after birth, possibly by regulating the expression of myelin proteins, such as MPZ, and by promoting the differentiation of Schwann cells. Involved in the development of the jaw openener musculature, probably by playing a role in its innervation through trigeminal motor neurons. May play a role in adipogenesis, possibly by regulating the expression of CEBPB.; E3 SUMO-protein ligase helping SUMO1 conjugation to its coregulators NAB1 and NAB2, whose sumoylation down-regulates EGR2 transcriptional activity.

Gene References into Functions

Low EGR2 expression is associated with hepatocellular carcinoma. PMID: 28542387
The study suggests that acetylation of EGR2 is regulated independently of nucleosome remodeling and deacetylase. PMID: 28576496
Egr2-driven cell surface proteins LAG-3 and 4-1BB can identify dysfunctional tumor antigen-specific CD8(+) TIL. PMID: 28115575
Egr2 and Egr3 have emerged as regulatory molecules that suppress excessive immune responses. Mice deficient for Egr2 and Egr3 develop a lupus-like disease with dysregulated activation of effector T cells. Egr2 and Egr3 confer suppressive activity to CD4(+) T cells and regulate the production of inhibitory cytokines such as IL-10 and TGF-beta1. PMID: 27856665
Analysis of consensus EGR-binding elements (EBEs) showed that the immediate early response 3 gene (IER3) is a novel transcriptional target gene of EGR2 as confirmed by the luciferase assay, electrophoretic mobility-shift assay (EMSA), chromatin immunoprecipitation (ChIP), and western blot analysis. PMID: 27890615
In the PPI network, genes may be involved in Down syndrome (DS) by interacting with others, including nuclear receptor subfamily 4 group A member 2 (NR4A2)early growth response (EGR)2 and NR4A2EGR3. Therefore, RUNX1, NR4A2, EGR2, EGR3 and ID4 may be key genes associated with the pathogenesis of DS. PMID: 27667480
EGR2 mutation presents as an axonal Charcot-Marie-Tooth phenotype with variable severity. PMID: 26204789
MicroRNA20a promotes the proliferation and cell cycle of human osteosarcoma cells by suppressing EGR2 expression. PMID: 26238942
These results suggested that EGR2 overexpression has a pivotal role in the downregulation of cytokines implicated in the pathophysiology of Guillain-Barre syndrome PMID: 26718337
A recurrent mutation was identified in EGR2 which appears to be associated with the pathogenesis of schizophrenia. PMID: 26119399
Knock-down of EGR2 with siRNA was demonstrated to have a similar effect as the over-expression of miR-330-3p in NSCLC cell lines PMID: 25935837
Dysregulated Egr-2 is observed in some human autoimmune disorders. PMID: 25381473
EGR2 knockdown inhibited proliferation, clonogenicity and spheroidal growth in vitro and induced regression of Ewing sarcoma xenografts. PMID: 26214589
transcription factor early growth response gene-2 is a novel molecular switch regulating known immunomodulatory molecules in human mesenchymal stem cells. PMID: 24007274
Overexpression of EGR2 significantly attenuated the oncogenic effect of miR-20a. PMID: 23924943
Lack of association of EGR2 variants with bipolar disorder in Japanese population. PMID: 23747400
EGR2 and EGR3 are regulated by NFkappaB and MAPK signalling pathways downstream of TNFalpha stimulation in breast adipose fibroblasts, and this in turn is upstream of CYP19A1 transcription via PI.4 PMID: 23485457
data suggest a tendency of gender-specific association of EGR2 and EGR4 in schizophrenia. PMID: 22691714
Charcot-Marie-Tooth neuropathy due to a novel EGR2 gene mutation exhibits a mild phenotype in a Czech family. PMID: 22546699
Egr2 expression is translationally regulated via an IRES element, which is responsive to an inflammatory environment. PMID: 22915601
we report on a heterozygous mutation in EGR2 in a family with a mild demyelinating form of adult-onset Charcot-Marie-Tooth disease PMID: 22734907
Combined with a plausible biological function of EGR2, the EGR2 gene is a possible susceptibility gene in bipolar disorder. PMID: 22089088
data further demonstrate the major role of the myelinating Schwann cell element in the regulation of EGR2 expression in the human peripheral nervous system PMID: 22522483
Results identify a mutation in EGR2 in a Charcot-Marie-tooth disease patient with hypersensitivity to vincristine. PMID: 22271166
Common variants near TARDBP and EGR2 are associated with susceptibility to Ewing sarcoma. PMID: 22327514
Krox20 functions as a SUMO ligase for its coregulators--the Nab proteins--and that Nab sumoylation negatively modulates Krox20 transcriptional activity in vivo. PMID: 21836637
TGF-beta3 regulated Egr-2 gene expression in uterine leiomyoma cells. PMID: 21703609
these findings suggest that Egr-2 plays an important nonredundant role in the pathogenesis of fibrosis. PMID: 21514423
EGR2 is a direct transcriptional target of p53 family that can in part mediate the p53-dependent apoptotic pathway. PMID: 21042708
Schwann cells expressed myelin proteins and Krox20 which is an important regulator of peripheral myelination. PMID: 21057508
EGR2 is a genetic risk factor for Systemic lupus erythematosus (SLE), in which increased gene expression may contribute to SLE pathogenesis. PMID: 20194224
EGR2 mutation(amino aacid substitution) identifies a case of Charcot-Marie-Totth disease. PMID: 20513111
overexpression of miR-150 in gastric cancer could promote proliferation and growth of cancer cells at least partially through directly targeting the tumor-suppressor EGR2. PMID: 20067763
Frequency of mutations in the early growth response 2 gene associated with peripheral demyelinating neuropathies PMID: 12471219
EGR2 mutant D355V induces a Cx32 promoter, mutant R381H doesn't. A sequence located at -216, recognized by the wild-type and the mutant D355V recombinant proteins, is relevant for promoter transactivation. PMID: 12609493
we found that EGR2 could induce apoptosis in a large proportion of these lines by altering the permeability of mitochondrial membranes, releasing cytochrome c and activating caspase-3, -8, and -9. PMID: 12687019
This report detected a heterozygous Asp383Tyr mutation of EGR2 in one patient with severe CMT1, Dejerine-Sottas syndrome. PMID: 12736090
a possible molecular mechanism to account for down-regulation of EGR2 in tumor cells PMID: 14596916
Egr-2 and Egr-3 transcription is enhanced by Hepatitis B virus X protein, which induces fasL gene expression PMID: 15173177
A Charcot-Marie-Tooth syndrome in a family with a missense mutation in EGR2. PMID: 15947997
One novel de-novo mutation (Arg359Gln, R359Q) was identified in heterozygous state in a patient with a typical CMT1 phenotype, progressive moderate thoracolumbar scoliosis and without clinical signs of cranial nerve dysfunction. PMID: 16198564
Control of Krox20 transcription relies on three very long-range enhancer elements (A, B and C) that are conserved in chick, mouse and human genomes. PMID: 16495311
morphological studies in the context of the I268N homozygous recessive mutation affecting the NAB repressor binding site and the R359W dominant-negative mutation in the zinc-finger domain PMID: 17717711
Collectively, these data indicate that EGR2 is specifically upregulated by a parasite-derived secreted factor that is most likely a resident rhoptry protein. PMID: 18678671
Dysregulation of EGR2 and MeCP2 plays roles in in Rett syndrome and autism. PMID: 19000991
AIP2 regulates activation-induced T-cell death by suppressing EGR2-mediated FasL expression via the ubiquitin pathway PMID: 19651900

Hide All

Involvement in disease

Neuropathy, congenital hypomyelinating or amyelinating (CHN); Charcot-Marie-Tooth disease 1D (CMT1D); Dejerine-Sottas syndrome (DSS)

Subcellular Location

Nucleus.

Protein Families

EGR C2H2-type zinc-finger protein family

Database Links

HGNC: 3239

OMIM: 129010

KEGG: hsa:1959

STRING: 9606.ENSP00000242480

UniGene: Hs.1395

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Code	CSB-RA196762A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: SY5Y whole cell lysate,U251 whole cell lysate,MCF7 whole cell lysate,K562 whole cell lysate,Mouse Brain tissue lysate,Mouse Brain tissue lysate All lanes: EGR2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50 kDa Observed band size: 50 kDa Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA196762A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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EGR2 Recombinant Monoclonal Antibody

Product Details

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Target Background