Recombinant Human Interferon-induced helicase C domain-containing protein 1(IFIH1),partial

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Code CSB-EP880143HUa2
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names IFIH1
Uniprot No. Q9BYX4
Research Area Epigenetics and Nuclear Signaling
Alternative Names CADM-140 autoantigen; Clinically amyopathic dermatomyositis autoantigen 140 kDa; DEAD/H (Asp Glu Ala Asp/His) box polypeptide; DEAD/H box polypeptide; Helicard; Helicase with 2 CARD domains; Hlcd; IDDM 19; IDDM19; IFIH 1; Ifih1; IFIH1_HUMAN; Interferon induced helicase C domain containing protein 1; interferon induced with helicase C domain 1; Interferon induced with helicase C domain protein 1; Interferon-induced helicase C domain-containing protein 1; Interferon-induced with helicase C domain protein 1; MDA 5; MDA-5; Melanoma differentiation associated protein 5; Melanoma differentiation-associated gene 5; Melanoma differentiation-associated protein 5; MGC133047; Murabutide down regulated protein ; Murabutide down-regulated protein; RH 116; RH116; RIG I like receptor 2; RLR 2; RNA helicase DEAD box protein 116; RNA helicase-DEAD box protein 116
Species Homo sapiens (Human)
Source E.coli
Expression Region 700-1025aa
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 53.5kDa
Protein Length Partial
Tag Info N-terminal 6xHis-SUMO-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include mRNA lacking 2'-O-methylation at their 5' cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases
Gene References into Functions
  1. RIG-I and MDA5 receptor sensing of host non-coding RNAs facilitates the cell-intrinsic immune response to Kaposi's sarcoma-associated herpesvirus infection. PMID: 30451863
  2. Melanoma differentiation-associated gene 5 (MDA5) is a recently described autoantigen target in a subset of patients with dermatomyositis. Anti-MDA5 dermatomyositis is characterized by a unique mucocutaneous and systemic phenotype PMID: 29229575
  3. genetic association studies in population of children in Hungary: Data suggest that a genetic polymorphism in IFIH1 (rs1990760) is associated with seasonal variation in onset of type 1 diabetes in population studied; this polymorphism in IFIH1 may contribute to manifestation of type 1 diabetes primarily in summer. PMID: 28929635
  4. Anti-MDA5 antibody helps in the diagnosis of dermatomyositis, predicting cutaneous, musculoskeletal and pulmonary manifestations. PMID: 29380533
  5. Engineered Oncolytic Poliovirus PVSRIPO Subverts MDA5-Dependent Innate Immune Responses in Cancer Cells. PMID: 29997212
  6. This work validates the significant role of MDA5 in IFN signaling and HCV infection and proposes the first lncRNA inhibiting HCV replication by promoting the activation of MDA5 and mediating the association between MDA5 and HCV RNA, the study of which may shed light on the MDA5 function and treatment for hepatitis C patients. PMID: 29899107
  7. anti-MDA5 antibody related to muscle weakness, Gottron's sign or papules, mechanic's hand, V rash, skin ulcers, panniculitis, alopecia, arthritis/arthralgia, pneumomediastinum, and elevated CK in patients with dermatomyositis [meta-analysis] PMID: 28983955
  8. the identification of a pathogen-restricted immunodeficiency due to loss-of-function variants in IFIH1, which result in defective innate recognition of RNA viruses, preventing the activation of an efficient antiviral IFN response. PMID: 28716935
  9. The connection between the rs1990760 polymorphism and the expression level of type III IFNs. PMID: 28000722
  10. Oncostatin M induces RIG-I and MDA5 expression and enhances the double-stranded RNA response in fibroblasts. PMID: 28560754
  11. data highlight new function of ARRDC4 in innate immunity, contributing to the better understanding about regulation of MDA5 activation after EV71 infection, and also suggest ARRDC4 may serve as a potential target for intervention of EV71-induced inflammatory response. PMID: 28594402
  12. Both diseases (Neuroinflammation and the Singleton-Merten Syndrome) are part of the interferonopathy grouping and that the Arg822Gln mutation in IFIH1 can cause a spectrum of disease, including neurological involvement. PMID: 28475458
  13. Knockdown of PBRM1 in colon cancer cells increased the expression of two receptor genes (RIG-I and MDA5) and upregulated interferon (IFN)-related and inflammation-related gene signatures. PMID: 28940253
  14. Dengue virus infection of human dendritic cells drives follicular T helper cells formation via crosstalk of RIG-I and MDA5. PMID: 29186193
  15. study revealed that encapsidation of DI-RNA (defective interfering genomes) molecules within the measles virus nucleocapsid abolished their immunoactive properties. identified specific interactions of DI-RNAs with both RIG-I and LGP2 but not MDA5. PMID: 28768856
  16. An up-regulation of type I IFN-induced gene transcripts in all 5 patients tested and a heterozygous gain-of-function mutation in IFIH1 in each family were discovered in families with a Singleton-Merten syndrome phenotype. PMID: 28605144
  17. Data indicate autosomal recessive, homozygous MDA5 encoding gene IFIH1 mutation in the proband, and both parents and a brother were carriers of this mutation. PMID: 28606988
  18. this study shows that PACT facilitates RNA-induced formation of MDA5 oligomers in response to viruses PMID: 28760879
  19. Human peripheral blood mononuclear cells (PBMCs) and cell lines expressing the risk variant IFIH1(T946) exhibited heightened basal and ligand-triggered production of type I interferons. PMID: 28553952
  20. Mechanistically, West Nile virus NS1 targets RIG-I and melanoma differentiation-associated gene 5 (MDA5) by interacting with them and subsequently causing their degradation by the proteasome. PMID: 28659477
  21. The rare variant rs35667974, encoding an Ile923Val amino acid change in the IFIH1 gene protein product, is protective for psoriatic arthritis. PMID: 28501801
  22. This would suggest that the change in IP-10 is genotypically driven, while the change in IL-6 may be reflective of systemic lupus erythematosus (SLE) transition status. These data suggest that IFIH1 may contribute to SLE pathogenesis via altered inflammatory mechanisms. PMID: 28234905
  23. dynamic sumoylation and desumoylation of MDA5 and RIG-I modulate efficient innate immunity to RNA virus and its timely termination. PMID: 28250012
  24. GPATCH3 negatively regulates both RIG-I- and MDA5-mediated signaling. PMID: 28414768
  25. Data support a new model where an LGP2-MDA5 oligomer shuttles NS3 to the mitochondria to block antiviral signaling PMID: 28483922
  26. MDA-5 stimulation leads to endothelial dysfunction. PMID: 27130701
  27. LGP2 plays an essential role in activating interferon signaling against hepatitis C virus (HCV) infection by promoting MDA5 recognition of HCV pathogen-associated molecular patterns. PMID: 28090671
  28. These data confirm the pathogenicity of IFIH1 c.2465G>A p.(Arg822Gln) for Singleton-Merten syndrome and affirm the striking phenotypic heterogeneity of this disorder PMID: 28319323
  29. RNAs isolated from HCV-replicating cells triggered robust IFN-beta and IFN-lambda production through MDA5. PMID: 27655134
  30. meta-analysis of peer-reviewed articles from 2008 to 2015 and data from this study indicated an association between rs1990760 and Systemic lupus erythematosus (SLE) onset . This is the first association analysis on IFIH1 polymorphisms and SLE susceptibility in Brazilian populations. PMID: 27813554
  31. This study reviewed that Neurologic Phenotypes Associated with Mutations in IFIH1 in patients with Aicardi-Goutieres Syndrome. PMID: 27643693
  32. We provide herein the results of pathological examination including detailed description of the neuropathological hallmarks. To our knowledge, this the first detailed clinico-pathological description of a patient with an IFIH1 pathogenic variant. PMID: 26833990
  33. Our findings indicate that although STAT4 and IFIH1 SNPs are not associated with T1D in a Brazilian population, they might play a role in susceptibility to T1D on a larger worldwide scale PMID: 26782418
  34. Results describe a family with gain of function mutation associated with cutaneous manifestations of Aicardi-Goutieres and Singleton-Merten syndromes PMID: 26284909
  35. These results showed that mRNA levels of MDA5 and RIG-1 were significantly decreased and increased, respectively, in chronic hepatitis B patients when compared to healthy controls. PMID: 26485346
  36. Findings indicate that melanoma differentiation associated protein-5 (MDA5) may serve as a complementary role in the toll-like receptor3 (TLR3) activated suppression of neuroblastoma (NB). PMID: 26208481
  37. The MDA5 knockdown had no significant impact on the phosphorylation of eukaryotic translation initiation factor 2alpha triggered by CCCP, and MDA5 itself was not recruited to SGs, and the regulation of MDA5 in the SG response occurs downstream of eIF2alpha. PMID: 26351918
  38. A significantly higher frequency of anti-melanoma differentiation-associated gene 5 (MDA5) antibodies was observed in Chinese patients with polymyositis/dermatomyositis (PM/DM) than in Japanese patients. PMID: 25903820
  39. MDA5 mediates neuroprotection against cerebral ischemic injury. PMID: 26564103
  40. discusses the different risk and protective IFIH1 alleles in the context of recent structural and functional analysis that relate to MDA5 regulation of interferon responses PMID: 26385483
  41. The study proposes that the turnover kinetics of the ATPase domain enables the discrimination of self/non-self RNA by both RIG-I and MDA5. PMID: 26215161
  42. This review briefly summarizes the RLR system, and focuses on the relationship between LGP2 and MDA5, describing in detail how these two proteins work together to detect foreign RNA and generate a fully functional antiviral response. PMID: 25794939
  43. Single nucleotide polymorphism in IFIH1 gene is associated with type 1 diabetes. PMID: 25042601
  44. the ectopic expression of RIOK3 or a phosphomimetic MDA5-S828D mutation attenuated MDA5-mediated signaling. PMID: 25865883
  45. As a receptor for viral RNA, MDA5 until now has been linked to antiviral host defense, but these novel studies show unexpected effects in antifungal immunity as well. PMID: 25579795
  46. MDA5 plays important role in sensing HCV infection to trigger interferon response. PMID: 25463548
  47. L region antisense RNA of EMCV is a key determinant of innate immunity to the virus and represents an RNA that activates LGP2 associated MDA5 in virally-infected cells. PMID: 24550253
  48. These data demonstrate that EFTUD2 restricts Hepatitis C Virus infection mainly through an RIG-I/MDA5-mediated, JAK-STAT-independent pathway, thereby revealing the participation of EFTUD2 as a novel innate immune regulator. PMID: 25878102
  49. There is an association of the IFIH1 locus with susceptibility to type 1 diabetes mellitus in the Polish population. PMID: 25515714
  50. Data indicate that pattern recognition receptors RIG-I and MDA5 displace viral proteins bound to dsRNA in an ATP-dependent manner. PMID: 25891073
  51. Data reports an association between the rs1990760 polymorphism in MDA5 and EV71 infection in children in China. PMID: 24621100
  52. polymorphisms and clinical outcome of dengue virus infection PMID: 25850761
  53. Confirm the strong association between anti-MDA5 antibodies and cutaneous ulcers in dermatomyositis patients. PMID: 25331610
  54. Our study supports previous findings of an association between the rs1990760 polymorphism of IFIH1 and systemic lupus erythematosus and indicates that this SNP may also be associated with malar rash in lupus patients. PMID: 24719229
  55. Association of IFIH1 gene polymorphisms with dilated cardiomyopathy in a Chinese population PMID: 24960033
  56. essential to prevent early degradation of IRF-3, thereby sustaining IRF-3-dependent antiviral innate immunity PMID: 24800889
  57. A specific IFIH1 gain-of-function mutation causes Singleton-Merten syndrome. PMID: 25620204
  58. significant role in the defense against hepatitis C virus PMID: 25130193
  59. Anti-MDA5 antibodies are not uncommon European patients with polymyositis/dermatomyositis. PMID: 25151986
  60. IFIH1 did not reveal significant single-SNP associations with multiple sclerosis risk. PMID: 25288302
  61. These findings support the reliability of using commercially available recombinant MDA5 for detecting anti-MDA5 antibodies and confirm the association of these antibodies with RP-ILD in a large series of Mediterranean patients with DM. PMID: 24741583
  62. CXCL10 expression is induced by Hantaan virus infection through TLR3, RIG-I, and MDA-5 pathways correlated with the disease severity PMID: 24701034
  63. findings suggest that RIG-I and MDA5 associate with AU-rich RNA species originating from the mRNA of the measles virus L gene. PMID: 24743923
  64. LGP2 and MDA5 work together to detect viral RNA and generate a complete antiviral response. PMID: 25127512
  65. The A allele of the rs1990760 polymorphism in the IFIH1 gene is associated with protection for arterial hypertension in type 1 diabetic patients. PMID: 24386202
  66. study suggests that the IFIH1 mutations are responsible for the AGS phenotype due to an excessive production of type I interferon PMID: 24995871
  67. Experiments with paramyxovirus V protein-insensitive proteins revealed that the primary outcome of LGP2 interference is suppression of its ability to synergize with MDA5. PMID: 24829334
  68. we are unable to conclude whether the Thr946Ala SNP differentially affects IFIH1 function, at least in the context of IFN-alpha production in response to exposure to poly I:C. PMID: 24117221
  69. Gain-of-function mutations in IFIH1 cause a spectrum of human disease phenotypes associated with upregulated type I interferon signaling, including Aicardi-Goutieres syndrome. PMID: 24686847
  70. The IFIH1 gene appears to play a major role in the development of some autoimmune diseases, and it is, therefore, a candidate gene for T1DM. PMID: 24402011
  71. MERS-CoV 4a protein interacted with PACT in an RNA-dependent manner but not with RIG-I or MDA5. PMID: 24522921
  72. RIG-I and MDA-5 detection of viral RNA-dependent RNA polymerase activity restricts positive-strand RNA virus replication. PMID: 24039580
  73. Meta-analysis demonstrates that the IFIH1 rs1990760 T-allele confers susceptibility to autoimmune diseases, suggesting that the IFIH1 rs1990760 polymorphism might have no effect on Graves' disease and Addison's disease. PMID: 23734776
  74. Targeting of RIG-I and MDA5 could thus become a quintessential strategy to encounter heterogeneous cancers. PMID: 23390110
  75. TLR4 signaling, induced by LPS, increases the expression of melanoma differentiation-associated gene 5 (MDA5) and interferon (IFN)-stimulated gene 56 (ISG56) in U373MG human astrocytoma cells PMID: 23684765
  76. three IFIH1 SNVs were associated with systemic lupus erythematosus. PMID: 23535865
  77. Data show that LGP2 is able to synergize with melanoma differentiation associated gene-5 (mda-5) to render cells to induction by poly(I:C), but did not enhance retinoic acid-inducible gene-I (RIG-I) to induce type I interferon in response to poly(I:C). PMID: 23671710
  78. Overexpression of the MDA5 protein reverses the suppression of IRF3 activation caused by EV71 infection. PMID: 23650567
  79. Collectively, our in vitro and in vivo studies both support a critical role for MDA5 in the innate immune response against hepatitis B virus infection. PMID: 23926323
  80. RAVER1 specifically regulates MDA5 activity, revealing a mechanism of differential regulation of MDA5- and RIG-I-mediated innate antiviral response. PMID: 23390309
  81. The MDA-5 phenotype is frequently a clinical mimic of the antisynthetase syndrome and is not associated with rapidly progressive interstitial lung disease. PMID: 23436757
  82. study represents the first report demonstrating an association of the IFIH1 rs1990760 polymorphism with systemic lupus erythematosus susceptibility in a Chinese population PMID: 23108955
  83. our results establish a distinct role for IFIH1 in apoptosis, inflammation, and autoantibody production, and explain the molecular basis of these three risk alleles for SLE pathogenesis. PMID: 23441136
  84. The IFIH1 locus polymorphisms are unlikely to be associated with Addison's disease. PMID: 22789000
  85. MDA5 is the key sensor for the dsRNA replicative intermediate form of positive sense ssRNA viruses. PMID: 22797917
  86. Findings indicate that phosphatases PP1alpha and PP1gamma are key regulators of RIG-I and MDA5 antiviral signaling. PMID: 23499489
  87. The common IFIH1 single nucleotide polymorphism may modify the frequency of enterovirus RNA in blood of healthy children. PMID: 23144876
  88. MDA5 induced by polyinosinic-polycytidylic acid, in turn, increased the expression of a chemokine CXCL10. PMID: 22892369
  89. No IFIH1 polymorphism showed statistically significant association with susceptibility to type 1 diabetes or other related autoimmune disorders in Brazilian patients. PMID: 23246693
  90. Depleting MDA5 with siRNA prevented IFNB production despite poly I:C stimulation. Induction of MDA5 upon stimulation was greater in IFNB-producing cells. PMID: 23284052
  91. Study reports the crystal structure of MDA5 bound to double stranded RNA(dsRNA), which shows how, using the same domain architecture, MDA5 recognizes the internal duplex structure, whereas RIG-I recognizes the terminus of dsRNA. PMID: 23273991
  92. A TLR3-mediated upregulation of VEGF and its receptor subtype 2 (VEGF-R2) in human mesangial cells upon activation of viral receptors, is reported. PMID: 22864531
  93. These data show that MDA5 is essential to induce the interferon response after human metapneumovirus infection infection in human and mouse dendritic cells as well as in an experimental mouse model of infection. PMID: 23152520
  94. MDA5 is a polymerization-dependent signaling platform that uses the amyloid-like self-propagating properties of mitochondrial antiviral-signaling protein to amplify signaling. PMID: 23090998
  95. HLA-DRB1*0101/*0405 is associated with susceptibility to anti-MDA-5 antibody-positive dermatomyositis in the Japanese population. PMID: 22886382
  96. Overall, this indicates that RIG-I and MDA5 can both contribute to the recognition and control of Bluetongue virus infection. PMID: 22915805
  97. Data show that the presence of autoantibodies against melanoma differentiation-associated gene 5 (MDA-5) in dermatomyositis (CADM) patients was inversely associated with the population of their city of residence. PMID: 22192091
  98. MDA5 may be involved in innate immune reactions against H pylori and associate with glandular atrophy and intestinal metaplasia in patients with H pylori infection. PMID: 22461656
  99. We present 2 cases of MDA-5-associated dermato-pulmonary syndrome and provide a comprehensive review of available literature. [review] PMID: 22732950
  100. Triggering of the dsRNA sensors TLR3, MDA5, and RIG-I induces CD55 expression in synovial fibroblasts. PMID: 22590509

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Involvement in disease Diabetes mellitus, insulin-dependent, 19 (IDDM19); Aicardi-Goutieres syndrome 7 (AGS7); Singleton-Merten syndrome 1 (SGMRT1)
Subcellular Location Cytoplasm, Nucleus
Protein Families Helicase family, RLR subfamily
Tissue Specificity Widely expressed, at a low level. Expression is detected at slightly highest levels in placenta, pancreas and spleen and at barely levels in detectable brain, testis and lung.
Database Links

HGNC: 18873

OMIM: 182250

KEGG: hsa:64135

STRING: 9606.ENSP00000263642

UniGene: Hs.163173

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