Code | CSB-RA963423A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:5000 |
The production of the IFIH1 monoclonal antibody involves immunizing animals with a synthesized peptide derived from human MDA5. Following immunization, B cells were isolated and fused with myeloma cells to generate hybridomas. The hybridomas were screened for IFIH1 antibody production. The variable light and variable heavy domains of the IFIH1 monoclonal antibody were sequenced, and the synthesized gene was cloned into a vector. The IFIH1 monoclonal antibody gene-containing vector was then transfected into cells for cultivation, and the resulting IFIH1 recombinant monoclonal antibody was isolated and purified using affinity chromatography from the cell culture supernatant. This IFIH1 recombinant monoclonal antibody specifically reacts with both human and mouse IFIH1 samples and has been tested in both ELISA and WB applications.
The IFIH1 protein, also known as MDA5, is an intracellular pattern recognition receptor that plays a crucial role in the innate immune response against viral infections. It belongs to the RIG-I-like receptor (RLR) family and recognizes viral RNA, particularly dsRNA, which is a common feature of many RNA viruses. Upon recognition of viral RNA, IFIH1 undergoes conformational changes that lead to its activation and oligomerization, ultimately leading to the induction of type I interferon and pro-inflammatory cytokine production, which trigger an antiviral response in the infected cells and neighboring cells.
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