Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

KRT1

Uniprot No.

P04264

Research Area

Signal Transduction

Alternative Names

67 kDa cytokeratin; CK-1; CK1; Cytokeratin-1; Cytokeratin1; EHK; EHK1; Epidermolytic hyperkeratosis 1; EPPK; Hair alpha protein; K1; K2C1_HUMAN; Keratin; Keratin type II cytoskeletal 1; Keratin-1; Keratin1; KRT 1; Krt1; KRT1A; NEPPK; type II cytoskeletal 1; Type II keratin Kb1; Type-II keratin Kb1

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

2-644aa

Target Protein Sequence

SRQFSSRSGYRSGGGFSSGSAGIINYQRRTTSSSTRRSGGGGGRFSSCGGGGGSFGAGGGFGSRSLVNLGGSKSISISVARGGGRGSGFGGGYGGGGFGGGGFGGGGFGGGGIGGGGFGGFGSGGGGFGGGGFGGGGYGGGYGPVCPPGGIQEVTINQSLLQPLNVEIDPEIQKVKSREREQIKSLNNQFASFIDKVRFLEQQNQVLQTKWELLQQVDTSTRTHNLEPYFESFINNLRRRVDQLKSDQSRLDSELKNMQDMVEDYRNKYEDEINKRTNAENEFVTIKKDVDGAYMTKVDLQAKLDNLQQEIDFLTALYQAELSQMQTQISETNVILSMDNNRSLDLDSIIAEVKAQYEDIAQKSKAEAESLYQSKYEELQITAGRHGDSVRNSKIEISELNRVIQRLRSEIDNVKKQISNLQQSISDAEQRGENALKDAKNKLNDLEDALQQAKEDLARLLRDYQELMNTKLALDLEIATYRTLLEGEESRMSGECAPNVSVSVSTSHTTISGGGSRGGGGGGYGSGGSSYGSGGGSYGSGGGGGGGRGSYGSGGSSYGSGGGSYGSGGGGGGHGSYGSGSSSGGYRGGSGGGGGGSSGGRGSGGGSSGGSIGGRGSSSGGVKSSGGSSSVKFVSTTYSGVTR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

69.9 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The DNA coding sequence translated into the human KRT1 protein sequence (2-644aa) was fused with the N-terminal 6xHis tag sequence to form the recombinant DNA, which was inserted into an expression vector. The reconstructed expression vector was transformed into the E.coli for follow-up expression. The product underwent purification to obtain the recombinant human KRT1 protein with N-terminal 6xHis tag. The SDS-PAGE analysis determined its purity higher than 85%. After electrophoresis, a 66 kDa protein band was observed on the gel.

KRT1 is gene encoding a protein called Keratin, type II cytoskeletal 1 (also known as Keratin-1) in human and belongs to intermediate filament family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. Keratin-1 is expressed in terminally differentiating epidermis. Mutations in this gene have been associated with the variants of bullous congenital ichthyosiform erythroderma in which the palms and soles of the feet are affected.

Target Background

Function

May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.

Gene References into Functions

The authors report a large Italian family affected Palmoplantar Keratoderma and Charcot Marie Tooth disease. Two different mutated genes, KRT1 and MPZ were responsible for the two main clinical signs. Exome analysis detected two missense mutations, one in KRT1 and one in MPZ. PMID: 27639257
Results show that missense mutations exert dominant negative effects on the keratins K1/K10 protein structure by altering inter-chain interactions. PMID: 27421141
KRT1 and the specific polymorphism of KRT1 in this Chinese Han population are associated with autoimmune diseases SLE and SSc PMID: 29028840
Case Report: post-zygotic mosaicism of KRT/1o mutations in epidermolytic Ichthyosis. PMID: 27722766
KRT1 played an important role of maintaining epithelial barrier and its down-regulation in intestinal tissue was correlated with the progression of inflammatory bowel disease. PMID: 28111259
Report genetic/clinical spectrum of KRT1 mutations in keratinopathic ichthyosis. PMID: 26581228
demonstrated the presence of a genetic cutaneous mosaicism. Both patients carry the KRT1 pI479T substitution, but in the palmoplantar areas of one of them, only the mutated allele is expressed (hemizygous). This leads to highlight a new type of cutaneous mosaic, the palmoplantar mosaicism PMID: 25904304
In our study, the missense mutation in the proband and his mother with epidermolytic ichthyosis was a single amino acid difference in codon 478, which causes more serious skin manifestations PMID: 25808222
Complete structure of an epithelial keratin 1/keratin 10 dimer has been presented. PMID: 26181054
These findings indicate that exogenous FABP4 interacts with plasma membrane proteins, specifically CK1. PMID: 26343611
analysis of a heterozygous novel splice junction mutation in the 2B domain of KRT1 in a family with diffuse palmoplantar keratoderma PMID: 25429721
In ichthyosis with confetti, a causal de novo KRT1 mutation had a C-terminal frameshift, replacing 22 C-terminal AAs with an alternate 30-AA peptide. It distorted the IF network and mislocalized to the nucleus. Reversion occurred by mitotic recombination. PMID: 25774499
In HeLa cells transiently expressing C2GnT-M-GFP, knockdown of KRT1 does not affect Golgi morphology but leaves C2GnT-M outside of the Golgi, resulting in the formation of sialyl-T antigen. PMID: 25605727
Decreased levels of cytokeratin-1 is associated with breast cancer. PMID: 25073515
Hsp74, a potential bladder cancer marker, has direct interaction with keratin 1. PMID: 25050384
identified among endothelial antigens to which antibodies are produced during heart transplant rejection PMID: 23707440
Case Report/Letter: specific mutation in 2B domain of KRT1 gives rise to mild phenotype of epidermolytic hyperkeratosis mimicking ichthyosis bullosa of Siemens. PMID: 23623204
study reports 2 related women of Colombian origin, affected by a severe ichthyosis curth-macklin phenotype, who present a novel KRT1 mutation c.1577delG (p.Gly526Alafs*88) PMID: 22834809
Absence of Krt1 caused a prenatal increase in interleukin-18 (IL-18) and the S100A8 and S100A9 proteins, accompanied by a barrier defect and perinatal lethality. PMID: 23132931
Among Japanese patients with bullous congenital ichthyosiform erythroderma for which genetic diagnosis was determined, all showed mustations in KRT1 or KRT10. PMID: 23182068
Identification Keratin 1 as a cDDP-resistant protein in nasopharyngeal carcinoma cell lines. PMID: 22348822
High cytokeratin is associated with colorectal carcinogenesis. PMID: 21912905
we describe one Chinese family affected with EHK, type PS-1 (severe palmoplantar hyperkeratosis, type 1) and report a recurrent missense mutation (c.1436T>C) in the 2B rod domain of KRT1 in this family. PMID: 22250628
mutation analysis in patients with epidermolytic ichthyosis by direct sequencing of KRT1 and KRT10 genes; identified 14 different mutations, of which four have not been published previously PMID: 21271994
keratin 1 L12 domain mutations are associated with a milder epidermolytic ichthyosis phenotype with pronounced palmoplantar keratoderma, and without neonatal erythroderma and scaling. PMID: 20500210
Data demonstrate that genetic variants in the KRT1 interval contribute to quantifiable differences in the migration rates of keratinocytes isolated from different individuals. PMID: 17668073
alpha-keratin intermediate filaments have a low-density core as seen by cryoelectron microscopy PMID: 12064938
The humans hair Keratin 1 genes are each clustered in the genome and clusters are part of the large typeI epithelial keratin gene domains on chromosomes. PMID: 15797458
bullous congenital ichthyosiform erythroderma (BCIE) caused by a mutation in the 1A helix initiation motif of keratin 1. PMID: 16361731
A new genetic polymorphism has been detected, which is especially prevalent among the African-American population. PMID: 16417221
Allelic expression differences result from the cumulative contribution of multiple DNA sequence polymorphisms. PMID: 16789827
The 2 keratin 1 mutations are associated with tonotubular keratin, i.e. 'whorls' of aggregated keratin that form tubules as seen in transverse or in longitudinal sections PMID: 18795921
Mutation L437P in the 2B domain of keratin 1 causes diffuse palmoplantar keratoderma in a Chinese pedigree. PMID: 19470048
Keratin 1, an intermediate filament network component, is the binding partner of the lymphocytic choriomeningitis virus nucleoprotein. PMID: 19494018
Infection by HPV may alter the differentiation status of the epidermis, leading to delayed or absent expression of cytokeratin 1. PMID: 19515043

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Involvement in disease

Epidermolytic hyperkeratosis (EHK); Ichthyosis hystrix, Curth-Macklin type (IHCM); Keratoderma, palmoplantar, non-epidermolytic (NEPPK); Ichthyosis annular epidermolytic (AEI); Keratoderma, palmoplantar, striate 3 (SPPK3)

Subcellular Location

Cell membrane. Note=Located on plasma membrane of neuroblastoma NMB7 cells.

Protein Families

Intermediate filament family

Tissue Specificity

The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis.

Database Links

HGNC: 6412

OMIM: 113800

KEGG: hsa:3848

STRING: 9606.ENSP00000252244

UniGene: Hs.80828

Code	CSB-EP012503HU
Abbreviation	Recombinant Human KRT1 protein
MSDS	MSDS Datasheet
Size	US$306
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Recombinant Human Keratin, type II cytoskeletal 1 (KRT1)

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