Recombinant Human Lupus La protein(SSB)

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Code CSB-EP022705HU
Size US$1726Purchase it in Cusabio online store
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names SSB
Uniprot No. P05455
Research Area Epigenetics and Nuclear Signaling
Alternative Names Autoantigen La; La ; La autoantigen; La autoantigen homolog; La protein; La ribonucleoprotein; La ribonucleoprotein domain family member 3; LA_HUMAN; LARP3; Lupus La antigen; Lupus La protein; Lupus La protein homolog ; MGC118101; MGC93380; mRNA for autoantigen; OTTMUSP00000014043; RP23 273G23.1; Sjoegren syndrome type B antigen; Sjogren syndrome antigen B (autoantigen La); Sjogren syndrome antigen B; SS B; SS-B; SS-B/La protein ; SSB
Species Homo sapiens (Human)
Source E.coli
Expression Region 1-408aa
Target Protein Sequence MAENGDNEKMAALEAKICHQIEYYFGDFNLPRDKFLKEQIKLDEGWVPLEIMIKFNRLNRLTTDFNVIVEALSKSKAELMEISEDKTKIRRSPSKPLPEVTDEYKNDVKNRSVYIKGFPTDATLDDIKEWLEDKGQVLNIQMRRTLHKAFKGSIFVVFDSIESAKKFVETPGQKYKETDLLILFKDDYFAKKNEERKQNKVEAKLRAKQEQEAKQKLEEDAEMKSLEEKIGCLLKFSGDLDDQTCREDLHILFSNHGEIKWIDFVRGAKEGIILFKEKAKEALGKAKDANNGNLQLRNKEVTWEVLEGEVEKEALKKIIEDQQESLNKWKSKGRRFKGKGKGNKAAQPGSGKGKVQFQGKKTKFASDDEHDEHDENGATGPVKRAREETDKEEPASKQQKTENGAGDQ
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 62.8kDa
Protein Length Full Length
Tag Info N-terminal 6xHis-SUMO-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Q&A and Customer Reviews

 Q&A
Q:

We are interested in several small units of many different products.  However, we are only interested in products with a GST tag.  For those listed below that have a His tag, what is the expense and time associated with getting these in a GST tag?   CSB-EP022705HU His

A:
Thanks for your inquiry,Note: We use different codes for different tags now, so the codes of these proteins have been updated below.
Code: CSB-EP022705HUe0
Name: Recombinant Human Lupus La protein(SSB)
Expression Region: 1-408aa, Full Length
Tag Info: N-terminal GST-tag
Expression Sequence:

MAENGDNEKMAALEAKICHQIEYYFGDFNLPRDKFLKEQIKLDEGWVPLEIMIKFNRLNRLTTDFNVIVEALSKSKAELMEISEDKTKIRRSPSKPLPEVTDEYKNDVKNRSVYIKGFPTDATLDDIKEWLEDKGQVLNIQMRRTLHKAFKGSIFVVFDSIESAKKFVETPGQKYKETDLLILFKDDYFAKKNEERKQNKVEAKLRAKQEQEAKQKLEEDAEMKSLEEKIGCLLKFSGDLDDQTCREDLHILFSNHGEIKWIDFVRGAKEGIILFKEKAKEALGKAKDANNGNLQLRNKEVTWEVLEGEVEKEALKKIIEDQQESLNKWKSKGRRFKGKGKGNKAAQPGSGKGKVQFQGKKTKFASDDEHDEHDENGATGPVKRAREETDKEEPASKQQKTENGAGDQ


Shipping format: The default delivery form is liquid form. If the customer has demand for lyophilized form, please remark this requirement when placing order.
Optional service: Do you want aseptic processing and/or endotoxin removal for this protein (recommended for cell culture or in vivo use)? With our endotoxin removal service we guarantee the endotoxin level to be
less than 0.1ng/ug (1EU ug). Both aseptic processing and endotoxin removal services are free of charge however they must be requested when the order is placed.
Purity: Greater than 90% as determined by SDS-PAGE.

Target Data

Function Binds to the 3' poly(U) terminus of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation
Gene References into Functions
  1. A cytoplasmic isoform of La protein as well as LARPs 6, 4, and 1 function in mRNA metabolism and translation in distinct but similar ways, sometimes with the poly(A)-binding protein, and in some cases by direct binding to poly(A)-RNA. PMID: 28782243
  2. our data support a novel model, whereby cancer-associated La protein contributes to cisplatin resistance by stimulating the translation of anti-apoptotic factor Bcl2 in HNSCC cells. PMID: 27105491
  3. La protein is an important microprocessor component regulating miRNA processing efficiency by association with DGCR8 to regulate formation of the DGCR8-Drosha complex for miRNA processing. PMID: 29087193
  4. La functions as gatekeeper ensuring correct tRNA maturation and protecting the miRNA pathway from potentially functional tRNA fragments. PMID: 27345152
  5. Findings identified two novel SUMO acceptors sites on La and suggest that sumoylation of La alters its RNA-binding activity, which could influence a broad range of RNA processing steps where La's function has been implicated. PMID: 27224031
  6. Binding of double-stranded RNA involves less penetration into the RNA recognition motif (RRM2) binding pocket and more engagement with the unstructured C-terminus of the La C-terminal domain (CTD). PMID: 27959512
  7. At the 3' UTR, HUR facilitates circularization of the viral genome through interaction with another host factor, La, which is critical for replication. PMID: 26339049
  8. expression of nuclear hLa Ag induces thymic clonal deletion and tTreg selection, and lymphocytic infiltration of the lung is a consequence of La-specific CD4(+) T cell autoimmunity PMID: 25582858
  9. Authors provide a model whereby a novel interplay between RNA-binding, RNA chaperoning and AKT phosphorylation of La protein regulates CCND1 IRES-mediated translation. PMID: 25520193
  10. Primary Sjogren's patients with WSP form a benign subgroup, with a lower prevalence of anti-SSB and extraglandular manifestations. PMID: 24529195
  11. A beta turn in the human La protein is responsible for the interaction with the hepatitis C virus RNA. PMID: 24478427
  12. Total IgG levels correlate positively with autoantibodies La, Ro60 and Ro52 but not with RING or B-box domain antibodies of Ro52. PMID: 23554036
  13. The competition between the host factor (La) and the viral protein (NS3) for binding to HCV IRES might regulate the molecular switch from translation to replication of HCV. PMID: 22355520
  14. Our study demonstrates that La plays a critical role in regulating HCV replication by interacting with the GCAC motif. PMID: 23552417
  15. Sjogren syndrome antigen B (SSB)/La promotes global microRNA expression by binding microRNA precursors through stem-loop recognition PMID: 23129761
  16. Iron promotes the translation initiation of hepatitis C virus by stimulating the expression of eIF3A and La proteins. PMID: 22634302
  17. oxidants cause nuclear export of La/SSB protein and subsequent association of La/SSB with Nrf2 untranslated regions and ribosomes PMID: 22207702
  18. data indicate that the JAK2(V617F) mutation affects p53 response to DNA damage through the upregulation of La antigen and accumulation of MDM2 PMID: 21785463
  19. the structural elements required for La-mediated RNA chaperone activity PMID: 22203678
  20. La binds to domain IV of the hepatitis C virus RNA using an RNA recognition that is quite distinct from its mode of binding to RNAs with a 3' UUU(OH) trailer. PMID: 22009680
  21. function of the RNA-binding protein La in promoting tumor progression of head and neck squamous cell carcinoma PMID: 22016766
  22. Autoantigen La promotes efficient RNAi, antiviral response, and transposon silencing by facilitating multiple-turnover RNA-induced silencing complex catalysis. PMID: 22055194
  23. Overexpression of mouse or human La enhanced M-MuLV particle release in the absence of glycosylated gag. PMID: 21343359
  24. La protein binds to and mediates interaction of the 5' and 3' noncoding ends of the Japanese encephalitis virus genome. PMID: 21294637
  25. show elevated La protein expression in cervical cancer tissue and its correlation with aberrant CCND1 protein levels in cervical tumor tissue lysates PMID: 20856207
  26. There is a correlation between La protein and HBV mRNA and the expression of HBV protein. PMID: 17064465
  27. study found expression of La protein is induced by HCV infection, and this induced La protein-activated telomerase activity in a hepatoma cell line; HCV infection might be strongly related to telomerase activity in the liver through La protein induction PMID: 20497049
  28. nuclear trafficking by La affects the normal order of pre-tRNA processing PMID: 12049746
  29. molecular characterization of the human La protein.hepatitis B virus RNA.B interaction in vitro PMID: 12121976
  30. La autoantigen is required for the internal ribosome entry site-mediated translation of Coxsackievirus B3 RNA PMID: 12384597
  31. binds differentially to multiple sites within the 5' untranslated region of Coxsackievirus B3 RNA PMID: 12457960
  32. in addition to full-length La protein, both N- and C-terminal halves were able to interact with hepatitis C virus internal ribosome entry site in vivo. PMID: 12540850
  33. Dengue 4 virus minus strand 3'UTR RNA binds with La protein in human monocytes PMID: 12584332
  34. Results suggest that La ribonucleoproteins (RNP) exist in distinct states that differ in subcellular localization, serine 366 phosphorylation, and associated RNAs. PMID: 14636586
  35. La motif adopts an alpha/beta fold that comprises a winged-helix motif elaborated by the insertion of three helices PMID: 15004549
  36. La protein binds to the 5' end and with the 3' UTR of Dengue virus RNA; interacts with the dengue virus nonstructural proteins NS5 and NS3 PMID: 15084396
  37. NMR assignment and secondary structure of the La motif PMID: 15213463
  38. Composition of La/hepatitis B virus ribonucleoprotein particles as well as interacting cellular factors, are critical determinants in the regulation of the stability of the HBV RNA. PMID: 15302879
  39. RRM1 and La motifs bind pre-tRNA PMID: 15371415
  40. CK2 is responsible for La S(366) phosphorylation in vivo PMID: 15485924
  41. Nonphosphorylated SSB interacts with nucleolin at nucleolar sites involved in rRNA biogenesis. PMID: 15572691
  42. This protein is s host factors responsible for the regulation of the hepatitis C virus internal ribosome entry site. PMID: 15685555
  43. essential for efficient hepatitis C virus replication PMID: 15823607
  44. In vitro RNA chaperone activity pf SSB was studied. PMID: 15928345
  45. nuclear retention of peptidylglycine alpha-amidating monooxygenase (PAM) mRNA is lost upon expressing the La proteins that lack a conserved nuclear retention element, suggesting a direct association between PAM mRNA and La protein in vivo PMID: 16107699
  46. Anti-La antibodies bound to immunodominant epitopes of La within the NH(2)-terminus and the RNA recognition motif (RRM) region of apoptotic human cells PMID: 16320341
  47. Here, we show by chromatin immunoprecipitation (ChIP) that La is associated with pol III-transcribed genes in vivo PMID: 16344466
  48. Results report the crystal structure of the N-terminal domain of human La, consisting of La and RRM1 motifs, bound to RNA. PMID: 16387655
  49. Changes in salivary production rate are associated with aging and SSB antibodies. PMID: 16467037
  50. La protein undergoes nucleocytoplasmic shuttling whereas EBER1 and EBER2 noncoding RNA's are confined to the nucleus. PMID: 16682524
  51. In mice transgenic for the mutant form of La autoantigen, mutant La mRNAs are not only repressed and translated to mutant La protein but, unexpectedly, expression of mutant La favors the development of systemic autoimmunity. PMID: 16849479
  52. suggest that conserved elements mediate nuclear retention, nuclear export, and RNA-binding activities of the multifunctional La protein PMID: 17308035
  53. La autoantigen can interact with both the 3' and 5'UTRs of coxsackievirus B3 independently of the poly(A) tail and may share the same binding site on the La protein. PMID: 17346312
  54. Increased Ro/SSA 60 and La/SSB mRNA expression in minor salivary glands in primary Sjogren's syndrome (pSS) suggest that these these 2 autoantigens, but not Ro/SSA 52, are involved in the tissue-specific autoimmune response in pSS. PMID: 17552056
  55. Overexpression of La autoantigen is associated with neoplasms PMID: 17875783
  56. Results suggest that La protein is cleaved by granzyme B and N-terminal La fragment (27 kD) translocated to the cytoplasm, thus leading to a novel autoantibody production during granzyme B-mediated cytotoxicity. PMID: 17945310
  57. the autoantibodies to SSB/La may modulate neutrophils function in systemic lupus erythematosis PMID: 17985193
  58. The La-mediated suppression of HAV translation and stimulation of poliovirus translation implies unexpected mechanistic differences between viral internal ribosomal entry site elements. PMID: 18282467
  59. Identification of a conserved set of interactions with the last two nucleotides at the 3' end of the RNA ligand that are key to binding by the La protein. PMID: 18547518
  60. La supports the growth of nonsegmented negative-strand RNA viruses by both IFN suppression and a potentially novel IFN-independent mechanism PMID: 18550659
  61. the frequency of La/SSB antibodies was higher in sporadic systemic lupus erythematosus (P = 0.048) than in those with familial SLE, although this was not significant after application of Bonferroni's correction for the number of comparisons PMID: 19074175
  62. Using electrophoretic mobility-shift and UV cross-linking assays, as well as a yeast three-hybrid system, it was shown here that La protein binds to the 3' stem-loop (SL) structure of Japanese encephalitis virus genome. PMID: 19264640
  63. The authors show that La preferentially binds pre-tRNAs over processed tRNAs or 3' trailer products through coupled use of two sites: one on the La motif and another on the RRM1 beta-surface that binds elsewhere on tRNA. PMID: 19287396
  64. Nine of the 45 consecutive CHB cases (20%) were anti-Ro/La-negative with no known cause; they were less stable and complete than the anti-Ro/La positive cases PMID: 19567621
  65. inhibits tRNA 3' processing PMID: 11580243

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Subcellular Location Nucleus
Database Links

HGNC: 11316

OMIM: 109090

KEGG: hsa:6741

STRING: 9606.ENSP00000260956

UniGene: Hs.632535

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