Recombinant Human Neutrophil elastase(ELANE)

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Code CSB-EP007587HU
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names ELANE
Uniprot No. P08246
Research Area Signal Transduction
Alternative Names Bone marrow serine protease; ELA2; ELANE; Elastase 2; Elastase 2 neutrophil; Elastase neutrophil expressed; Elastase-2; ELNE_HUMAN; GE; Granulocyte derived elastase; HLE; HNE; Human leukocyte elastase; Leukocyte elastase; Medullasin; NE; Neutrophil elastase; PMN E; PMN elastase; Polymorphonuclear elastase; SCN1
Species Homo sapiens (Human)
Source E.coli
Expression Region 30-267aa
Target Protein Sequence IVGGRRARPHAWPFMVSLQLRGGHFCGATLIAPNFVMSAAHCVANVNVRAVRVVLGAHNLSRREPTRQVFAVQRIFENGYDPVNLLNDIVILQLNGSATINANVQVAQLPAQGRRLGNGVQCLAMGWGLLGRNRGIASVLQELNVTVVTSLCRRSNVCTLVRGRQAGVCFGDSGSPLVCNGLIHGIASFVRGGCASGLYPDAFAPVAQFVNWIDSIIQRSEDNPCPHPRDPDPASRTH
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 52.6kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal GST-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis.
Gene References into Functions
  1. Results found that the neutrophil elastase is up-regulated in both overweight and obese subjects and associated with BMI and markers of cardiovascular disease. PMID: 30056589
  2. Munc134 mediates human neutrophil elastaseinduced airway mucin5AC hypersecretion by interacting with syntaxin2. PMID: 29767240
  3. Neutrophil elastase impaired the EMILIN1-alpha4beta1 integrin interaction by cleaving the gC1q domain in a region crucial for its proper structural conformation, paving the way to better understand NE effects on EMILIN1-cell interaction in pathological context. PMID: 28074935
  4. the effect of elastase on gammadelta T cells was mediated through the protease-activated receptor, PAR1, because the inhibition of this receptor with a specific antagonist, RWJ56110, abrogated the effect of neutrophils on gammadelta T-cell activation. PMID: 28888033
  5. PMN elastase is released by neutrophils in response to Streptococcus pneumoniae pneumolysin. PMID: 27892542
  6. Plasma Neutrophil Elastase and Elafin as Prognostic Biomarker for Acute Respiratory Distress Syndrome. PMID: 28187039
  7. Our results suggest that associated serum levels of proMMP-9, NGAL, proMMP-9/NGAL and NE may reflect the state of systemic inflammation in chronic obstructive pulmonary disease related to cigarette smoking. PMID: 28004483
  8. Using different blood leukocyte and skin resident cell preparations, and recombinant proteins, the authors have identified that neutrophil elastase, but not other neutrophil derived proteases, cleaves IL-36Ra into its highly active antagonistic form. PMID: 27101808
  9. Taken together, these observations support that the unusual HNE N-glycosylation, here reported for the first time, is involved in modulating multiple immune functions central to inflammation and infection. PMID: 28630087
  10. results suggest activation of elastase in tendinopathy of the long head of the biceps increases the degradation of elastin to increase the levels of elastase, becoming a vicious cycle to the progressive severity of tendinopathy PMID: 27935111
  11. Coincubation with the inhibitors, particularly MK0339, promoted cell survival and increased formation of mature neutrophils. These studies suggest that cell-permeable inhibitors of neutrophil elastase show promise as novel therapies for ELANE-associated neutropenia. PMID: 28754797
  12. Data suggest that neuropilin-1 (NRP1) functions as a receptor for neutrophil elastase (NE), mediates uptake/absorption of NE, and facilitates cross-presentation of PR1 (a peptide fragment of NE) in breast cancer cells. PMID: 28468826
  13. the impact of NE mutations that are typical of human congenital neutropenia in a murine model of in vitro and in vivo neutrophil differentiation PMID: 27942017
  14. Faecal biomarkers lactoferrin, calprotectin, and PMN-elastase were able to distinguish between ulcerative colitis patients with mucosal healing from clinical remission and mild disease, showed significant correlations with endoscopy, and were predictive of a flare. PMID: 26874351
  15. In conclusion, site-specific CBG N-glycosylation regulates the bioavailability of cortisol in inflamed environments by fine-tuning the RCL proteolysis by endogenous and exogenous elastases. PMID: 27339896
  16. Although circulating nucleosomes and elastase - alpha 1-antitrypsin complexes are at present the best characterized biomarkers for neutrophil extracellular traps formation and neutrophil activation, their lack of association with rs2288904 genotype does not exclude a role for SLC44A2 in neutrophil activation (and possibly NET formation) in Venous Thromboembolism. PMID: 27093231
  17. The levels of both NE and PR3 correlated with absolute neutrophil counts and are reduced in type 1 diabetes mellitus patients. PMID: 26939803
  18. The results of the present study may indicate that NE activates the phosphoinositide-3 kinase/Akt signaling pathway in leukemia cells to inhibit apoptosis and enhance cell proliferation PMID: 27035679
  19. The HNE-TACE signalling pathway has an important role in the process of MUC5AC overexpression in chronic rhinosinusitis. PMID: 26881964
  20. An increased NE: A1 AT ratio is closely associated with liver Inflammation in patients with NASH and could serve as a novel marker to predict NASH in humans PMID: 26444279
  21. Neutrophil elastase activity is deleterious for airway epithelial cell homeostasis. PMID: 26221769
  22. mosaic mutations result in cyclic neutropenia PMID: 25912133
  23. insights into the PPE and HNE inhibition PMID: 26372354
  24. Different ELANE mutants show specific activation of different UPR pathways in congenital neutropenia. PMID: 26567890
  25. Neutrophil elastase was increased in the saliva from COPD patients and additionally distinguished smoking status. PMID: 26018813
  26. Paternal Somatic Mosaicism of a Novel Frameshift Mutation in ELANE Causing Severe Congenital Neutropenia. PMID: 26174650
  27. Sirtinol is a novel HNE inhibitor and may have the potential for clinical application in the treatment of inflammatory lung diseases. PMID: 25666548
  28. Autoprocessing of neutrophil elastase near its active site reduces the efficiency of natural and synthetic elastase inhibitors. PMID: 25857284
  29. analysis of the mechanistic link between neutrophil elastase expression in atherosclerotic plaques and concomitant pro-inflammatory bioactive IL-1beta secretion from endothelial cells PMID: 26269588
  30. In patients with carotid plaques, both circulating and intraplaque polymorphonuclear neutrophils produce IL-8, VEGF and elastase, which are crucial for plaque development and progression. PMID: 25893670
  31. Pathogenesis of ELANE-mutant severe neutropenia revealed by induced pluripotent stem cells. PMID: 26193632
  32. The imbalance between Neutrophil elastase and its inhibitors, such as elafin, presents an important therapeutic target in breast cancer. PMID: 25195861
  33. Neutrophil elastase mainly enhances tissue factor production by monocytes via the phospholipase C/conventional PKC/p38 MAPK pathway, although a novel PKC is also involved. PMID: 25465717
  34. results suggest that elastase-like proteases in the microglial cells surrounding Abeta plaques are responsible for ApoE degradation in the brain. PMID: 25956321
  35. PAR2 and TRPV4 are activated by neutrophil elastase to cause inflammation and pain PMID: 25878251
  36. Three proteins-matrix metalloproteinase-9, neutrophil elastase, and FAM49B-were significantly lower in abundance in samples from women with endometriosis. PMID: 25284053
  37. Sequencing is useful for predicting outcomes of ELANE-associated neutropenia. PMID: 25427142
  38. Over-expressed NE in K562 leukemia cells promotes cell proliferation, inhibits apoptosis and block cell cycle in S phase. PMID: 25652853
  39. Suggest a potential role of ELANE gene polymorphisms as therapy modulators in childhood bronchiectasis. PMID: 24816969
  40. An interactive network of elastase, secretases, and PAR-2 protein regulates CXCR1 receptor surface expression on neutrophils PMID: 24914212
  41. Circulating protein levels and enzymatic activity of ELANE are markedly elevated in patients with type 1 diabetes mellitus, and are correlated with increased autoantibodies against beta-cell antigens. PMID: 25092677
  42. The present findings highlight the peculiar activity of PMN elastase in disabling EMILIN1 suppressor function. PMID: 24513040
  43. It reports the synthesis and enzymatic studies on a new proteinase 3 intermolecular quenched substrate with enhanced selectivity over neutrophil elastase. PMID: 23911525
  44. The leukocyte elastase gene is substituted at the 597th base (G to A) in intron 4 of severe congenital neutropenia. PMID: 24616599
  45. Human sex hormone-binding globulin mediates the targeted release of cortisol at sites of inflammation through cleavage of its reactive center loop by neutrophil elastase. PMID: 24848868
  46. neutrophil elastase reflects the severity and progression of acute pancreatitis. PMID: 23819644
  47. ELANE mutations disrupt translation initiation and are associated with neutropenia; they produce novel neutrophil elastase isoforms PMID: 24184683
  48. SPLUNC1 degradation by neutrophil elastase may increase airway susceptibility to bacterial infections. PMID: 23741370
  49. Neutrophil elastase and proteinase-3 trigger G protein-biased signaling through proteinase-activated receptor-1 (PAR1). PMID: 24052258
  50. Specific ELANE mutations have limited predictive value for leukemogenesis; the risk for leukemia was correlated with disease severity rather than with occurrence of an ELANE mutation. PMID: 23463630
  51. 4 ELANE mutations, 11 HAX1 mutations and 2 G6PC3 mutations have been identified in Iranian patients with severe congenital neutropenia. PMID: 23454784
  52. Overexpression of G185R ELA2 mutant increases apoptosis through an unfolded protein response. PMID: 22996420
  53. Neutrophil elastase can increase PAR2 expression and MUC5AC release. PMID: 23392769
  54. A review discusses how ELANE mutations provide clues into the pathogenesis of cyclic and severe congenital neutropenia. PMID: 23351986
  55. Human neutrophil elastase induced goblet cell metaplasia in the wild-type mice and that HNE-induced goblet cell metaplasia was significantly attenuated in the Tace conditional deletion mice. PMID: 23564510
  56. Neutrophil elastase activity in BAL fluid in early life was associated with early bronchiectasis in children with cystic fibrosis. PMID: 23692169
  57. Chamomile infusion inhibited expression of neutrophil elastase and matrix metalloprotease-9 in adenocarcinoma gastric cells. PMID: 22407864
  58. E-cadherin expression correlated negatively with PMN infiltration, compatible with the notion that E-cadherin is cleaved by PMN-derived elastase PMID: 23001948
  59. Neutrophil extracellular traps-associated elastase digested amyloid fibrils into short species that were cytotoxic for BHK-21 and HepG2 cells. PMID: 22918834
  60. Variations in SERPINA1 and ELA2 were not consistently or strongly associated with the risk of either COPD or NSCLC in either African Americans or caucasian. PMID: 22993338
  61. High elastase expression is associated with defective alveolar septation and arrested lung growth. PMID: 22683569
  62. Report on development of pulmonary emphysema after intratracheal instillation of human neutrophil elastase. PMID: 22500123
  63. In a process reminiscent of neutrophil extracellular traps formation, NE enhances sputum solubilization. PMID: 22174830
  64. ELANE genetic polymorphism is associated with development of periodontitis in patients with severe congenital neutropenia PMID: 21796505
  65. Tagetes erecta flower showed effective inhibition of leukocyte elastase. PMID: 21843618
  66. a new mode of PAR regulation that involves biased PAR(2) signaling by neutrophil elastase and a disarming/silencing effect of cathepsin-G and proteinase-3. PMID: 21576245
  67. Plasma neutrophil elastase may be involved in the pathogenesis of severe pneumonia. PMID: 21605276
  68. heparin is able to accelerate the hydrolysis of TIMP-1 by HNE. PMID: 21731773
  69. catalyses inactivation of factor VIII(a)via limited proteolysis PMID: 21475777
  70. Immature dendritic cells treated with neutrophilic elastase or polymorphonuclear culture supernatant, promote the generation of CD4+FOXP3+ Tregs in vitro. PMID: 21477798
  71. Four novel heterozygous mutations in the ELANE gene in patients with congenital neutropenia PMID: 21425445
  72. The study shows that the alternative pathway for plasmin-mediated fibrinolysis by leukocyte elastase is activated, and contributes to the degradation of cross-linked fibrin in sepsis-induced disseminated intravascular coagulation. PMID: 21236472
  73. SCN with the identification of a mutation in the ELANE gene. PMID: 21448199
  74. variants of alpha-1-antitrypsin and ELA2 genotypes with predicted intermediate or high activity could represent a risk factor for aggressive form of lung cancer PMID: 20521180
  75. MBP and NE collaborated to cause the pathological effects of nasal polyps. PMID: 18476621
  76. Human neutrophil elastase treatment does not modify short-circuit current in control or nasal epithelial cells from cystic fibrosis patients. PMID: 20932306
  77. The majority of patients with severe congenital neutropenia bear monoallelic mutations in the neutrophil elastase (ELANE) gene PMID: 21206270
  78. NE is the predominant protease that cleaves hemoglobin in vivo in cystic fibrosis bronchoalveolar lavage fluid PMID: 21193404
  79. Findings of significantly elevated serum MMP-9, myeloperoxidase, and neutrophil elastase together with decreased TIMP-1 in smoker periodontitis patients provides support for the smoking associated risk of cardiovascular diseases in periodontitis patients PMID: 20646231
  80. Results describe a multigenerational family with mutations in ELANE as unambiguous cause of severe congenital neutropenia. PMID: 20803142
  81. NE significantly promotes the proliferation of HaCaT cell. Meanwhile, it also up-regulates the expression levels of Ki67 and p53 in psoriasis lesion tissue, which plays an important role in the pathogenesis of psoriasis lesion. PMID: 20883273
  82. The neutrophil elastase (NE) escapes from azurophilic granules and translocates to the nucleus, where it partially degrades specific histones, promoting chromatin decondensation. PMID: 20974816
  83. Cluster analysis reveals two classes of thrombi according to neutrophil elastase -specific fibrin degradation products (NE-FDP),leukocyte and platelet content and also two according to NE-FDP, leukocyte and fibrin content. PMID: 20627279
  84. elevated in chronic compared to acute wounds PMID: 20033193
  85. The trafficking of precursor NE (proNE) and precursor PR3 (proPR3), was compared. PMID: 20828556
  86. Results describe the direct effects of the antibiotics colistin and tobramycin on neutrophil elastase activity in cystic fibrosis patients. PMID: 20634941
  87. NE digests ferritin increasing the extracellular iron pool available for cellular uptake PMID: 20411049
  88. Sivelestat may attenuate neutrophil elastase or proinflammatory cytokines, and improve pulmonary dysfunction in patients undergoing cardiopulmonary bypass. PMID: 20354035
  89. Sputum samples from cystic fibrosis patients had elevated human neutrophil elastase (HNE) levels which correlated with an increased MMP-9/TIMP-1 ratio. PMID: 20111696
  90. coexistence of CN and SCN phenotypes in this kindred with a shared S97L ELANE paternal haplotype strongly suggests both a role for modifying genes in determination of congenital neutropenia disease phenotypes PMID: 20582973
  91. In patients in preterm labor with a high elastase concentration in cervical secretions, treatment with urinary trypsin inhibitor reduced the risk of preterm delivery and improved neonatal outcomes. PMID: 20453419
  92. Neutrophil elastase inhibitor, Sivelestat, significantly reduced the postoperative decreases in AT III and platelet count in patients undergoing emergency surgery for acute aortic dissection. PMID: 20376727
  93. no consistent pattern among sites in the vagina in pelvic organ prolapse PMID: 19763368
  94. REVIEW: structural basis for the different ligand specificities and membrane binding mechanisms of human proteinase 3 and neutrophil elastase PMID: 20423453
  95. ELA2 as a major new epidermal protease involved in essential pathways for skin barrier function. PMID: 20179351
  96. Secretory leucoprotease inhibitor is cleaved and inactivated by neutrophil elastase present in Pseudomonas aeruginosa-positive secretions in the cystic fibrosis lung. PMID: 20007580
  97. Mutant neutrophil elastase induces accelerated apoptosis of promyelocytes, leading to maturation arrest in congenital and cyclic neutropenia. PMID: 11846296
  98. UVA light stimulates the production of neutrophil elastase by dermal fibroblasts: a possible contribution to the remodeling of elastotic areas in sun-damaged skin. PMID: 11928814
  99. Elastase is mainly responsible for cartilage damage by stimulated human neutrophils, as demonstrated by the use of selective elastase inhibitors. PMID: 12020136
  100. Neutrophil elastase-induced overexpression of mucin genes in cultured tumor cells is inhibited by a retinoic acid receptor alpha antagonist. PMID: 12042033

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Involvement in disease Cyclic haematopoiesis (CH); Neutropenia, severe congenital 1, autosomal dominant (SCN1)
Protein Families Peptidase S1 family, Elastase subfamily
Tissue Specificity Bone marrow cells.
Database Links

HGNC: 3309

OMIM: 130130

KEGG: hsa:1991

STRING: 9606.ENSP00000263621

UniGene: Hs.99863

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