Greater than 90% as determined by SDS-PAGE.
Cell migration-inducing gene 10 protein; Epididymis secretory sperm binding protein Li 68p; HEL S 68p; MGC117307; MGC8947; MIG10; pgk1; PGK1_HUMAN; PGKA; Phosphoglycerate kinase 1; Primer recognition protein 2; PRP 2
Homo sapiens (Human)
Target Protein Sequence
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
3-7 business days
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
The recombinant Human PGK1 protein synthesis in e.coli cells necessitates the incorporation of a DNA fragment encoding the Human PGK1 protein (25-417aa) into a plasmid vector, followed by the transformation of this vector into e.coli cells. After screening for positive cells, they are cultured and induced to express the PGK1 protein. The protein carries a N-terminal GST tag. Cell lysis is performed to gather the recombinant Human PGK1 protein, which undergoes affinity purification and is then analyzed using SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of the resulting recombinant Human PGK1 protein reaches up to 90%.