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The gene fragment corresponding to the 1-442aa of the human CRBN protein was synthesized, with appropriate restriction sites suitable for in-frame cloning into an expression vector, with C-terminal 9xHis tag. The Baculovirus was transformed with the expression vector, and the clone was expressed upon certain induction. After the induced cell centrifugation, the recombinant protein was purified from the cell extract and presented as C-terminal 9xHis-tagged fusion. This recombinant human CRBN protein's purity is greater than 85% assayed by SDS-PAGE. The CRBN protein ran to a band of about 56 kDa molecular weight on the gel, indicating a glycosylated form of the protein.
Cereblon (CRBN) is a common direct target of thalidomide and related compounds and works as a Cullin Ring 4 E3 ubiquitin ligase (CRL4) with DDB1, CUL4, and ROC1. CRBN the molecular target of lenalidomide and pomalidomide, is a substrate receptor of the cullin ring E 3 ubiquitin ligase complex, CRL 4 CRBN. In early reports, Researchers identifed that a novel cereblon modulator recruits GSPT1 to the CRL4-CRBN ubiquitin ligase. The substrate specificity of CRL4CRBN is modulated by thalidomide-related compounds. Ablation ofCRBNinduces loss of type I collagen and SCH in mouse skin by fibroblast senescence via the p38 MAPK pathway. The CRBN, CUL4A and DDB1 expression predicts the response to immunomodulatory drugs and survival of multiple myeloma patients. Cereblon modulator CC-885 inducesCRBN-dependent ubiquitination and degradation of CDK4 in multiple myeloma.
|Purity||Greater than 85% as determined by SDS-PAGE.|
CRBN; AD-006; Protein cereblon
|Species||Homo sapiens (Human)|
|Target Protein Sequence||MAGEGDQQDAAHNMGNHLPLLPAESEEEDEMEVEDQDSKEAKKPNIINFDTSLPTSHTYLGADMEEFHGRTLHDDDSCQVIPVLPQVMMILIPGQTLPLQLFHPQEVSMVRNLIQKDRTFAVLAYSNVQEREAQFGTTAEIYAYREEQDFGIEIVKVKAIGRQRFKVLELRTQSDGIQQAKVQILPECVLPSTMSAVQLESLNKCQIFPSKPVSREDQCSYKWWQKYQKRKFHCANLTSWPRWLYSLYDAETLMDRIKKQLREWDENLKDDSLPSNPIDFSYRVAACLPIDDVLRIQLLKIGSAIQRLRCELDIMNKCTSLCCKQCQETEITTKNEIFSLSLCGPMAAYVNPHGYVHETLTVYKACNLNLIGRPSTEHSWFPGYAWTVAQCKICASHIGWKFTATKKDMSPQKFWGLTRSALLPTIPDTEDEISPDKVILCL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
|Mol. Weight||52.5 kDa
|Protein Length||Full Length|
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
|Buffer|| If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
|Reconstitution||We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.|
|Storage Condition||Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.|
|Shelf Life||The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
|Lead Time||3-7 business days|
|Notes||Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.|
|Datasheet & COA||Please contact us to get it.|
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Does it exist in its properly folded state such that it is biologically active? What buffer is being used?
I have one more questions about CSB-BP842761HU protein.
What is the concentration of the remaining 90ug product?
Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2 (Probable). Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8. Maintains presynaptic glutamate release and consequently cognitive functions, such as memory and learning, by negatively regulating large-conductance calcium-activated potassium (BK) channels in excitatory neurons. Likely to function by regulating the assembly and neuronal surface expression of BK channels via its interaction with KCNT1. May also be involved in regulating anxiety-like behaviors via a BK channel-independent mechanism.
|Gene References into Functions||
|Involvement in disease||Mental retardation, autosomal recessive 2A (MRT2A)|
|Subcellular Location||Cytoplasm. Nucleus. Membrane; Peripheral membrane protein.|
|Protein Families||CRBN family|
|Tissue Specificity||Widely expressed. Highly expressed in brain.|