Recombinant Human Ras-related protein R-Ras (RRAS)

Code CSB-YP020513HU
MSDS
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Source Yeast
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Code CSB-EP020513HU
MSDS
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Source E.coli
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Code CSB-EP020513HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP020513HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP020513HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RRAS
Uniprot No.
Alternative Names
Oncogene RRAS; p23; R Ras; Ras related Protein ; Ras related protein R Ras; Ras-related protein R-Ras; related RAS viral (r ras) oncogene homolog ; RRAS; RRAS_HUMAN
Species
Homo sapiens (Human)
Expression Region
1-215
Target Protein Sequence
MSSGAASGTG RGRPRGGGPG PGDPPPSETH KLVVVGGGGV GKSALTIQFI QSYFVSDYDP TIEDSYTKIC SVDGIPARLD ILDTAGQEEF GAMREQYMRA GHGFLLVFAI NDRQSFNEVG KLFTQILRVK DRDDFPVVLV GNKADLESQR QVPRSEASAF GASHHVAYFE ASAKLRLNVD EAFEQLVRAV RKYQEQELPP SPPSAPRKKG GGCPC
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Regulates the organization of the actin cytoskeleton. With OSPBL3, modulates integrin beta-1 (ITGB1) activity.
Gene References into Functions
  1. R-Ras activation is associated melanoma tumorigenesis. PMID: 26993606
  2. this study identified potential biochemical players involved in distant recurrence and indicates that R-Ras and Transgelin are potential post-surgical prognostic biomarkers for Stage III colorectal cancer PMID: 27270312
  3. RRAS is a key regulator of miR-34/449 promotion of actin network formation during multiciliogenesis. PMID: 26381333
  4. Evidence is provided of a functional link between RRAS and MAPK signalling and reveal an unpredicted role of enhanced RRAS function in human disease. PMID: 24705357
  5. Girdin regulates the trafficking of VE-cadherin in synergy with R-Ras. PMID: 25869066
  6. phosphorylation of ORP3 controls its association with VAPA. Furthermore, ORP3-VAPA complexes stimulate R-Ras signaling. PMID: 25447204
  7. Contradicting the positive association reported in previous studies, our results indicate that R-Ras activation may negatively regulate the transformation of breast epithelial cells. PMID: 24700169
  8. A polymorphism linked to RRAS, SCAF1, IRF3 and BCL2L12 genes is associated with cirrhosis in hepatitis C virus carriers. PMID: 24131527
  9. Patients with gemistocytic astrocytomas with an RRAS deletion tend to have shorter survival rates than those without deletion. PMID: 25192052
  10. These data suggest that pharmacological inhibition of RRAS signaling may confer therapeutic benefit in Huntington's disease. PMID: 23209424
  11. These findings identify R-Ras as a critical regulator of vessel integrity and function during tumor vascularization. PMID: 22897853
  12. Maintaining endothelial barrier function is dependent upon active R-Ras and association between R-Ras and FLNa. PMID: 21660952
  13. Data suggest that the RRAS gene is an important regulatory module in the pathogenesis of biliary atresia (BA), which may serve as a novel prognostic marker for BA. PMID: 21390152
  14. data support a model in which R-Ras functionally associates with FLNa and thereby regulates integrin-dependent migration PMID: 20585650
  15. Sema3E acts on plexin D1 to initiate a two-pronged mechanism involving R-Ras inactivation and Arf6 stimulation, which affect the status of activation of integrins and their intracellular trafficking, respectively. PMID: 20385769
  16. study of the potential contribution in generating of estrogen-independence in MCF-7 cells PMID: 12386818
  17. the oncogene R-Ras promotes tumor growth of cervical epithelial cells and increases their migration potential over collagen through a pathway that involves PI 3-K. PMID: 12548599
  18. activation of R-Ras promotes the formation of focal adhesions and a spread cell shape PMID: 12890755
  19. Plexin-B1 mediates Sema4D-induced repulsive axon guidance signaling by acting as a GTPase activating protein for R-Ras PMID: 15297673
  20. R-Ras plays a key role in cell migration by locally regulating the switch from Rac to Rho activity after membrane protrusion and adhesion PMID: 15525681
  21. GPCRs rapidly activate R-Ras; R-Ras activation by the GPCRs is apparently directly induced by cyclic AMP-regulated Epac proteins; and activated R-Ras specifically controls GPCR-mediated phospholipase D stimulation PMID: 16754664
  22. These findings show that R-Ras activity mediates inhibition of insulin signaling associated with suppression of estrogen action, implicating this GTPase in a growth-inhibitory mechanism associated with antiestrogen treatment of breast cancer. PMID: 16914723
  23. R-Ras and ILK have roles upstream of GSK-3beta in the regulation of neuronal polarity PMID: 17107957
  24. Notch1 ligand, Delta-like ligand-4, stimulates R-Ras-dependent alpha 5 beta 1 integrin-mediated adhesion, demonstrating the physiological relevance of this pathway. PMID: 17664272
  25. results map the functional elements in the R-ras promoter sequence and suggest that the GABP may be critical for transcription of R-ras and for maintenance of normal blood vessel functions through the regulation of this gene PMID: 19011236
  26. TCF8 activates R-Ras, another class of angiogenic regulator, to suppress angiogenesis by a mechanism other than a transcriptional attenuator. PMID: 19116136

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Subcellular Location
Cell membrane; Lipid-anchor; Cytoplasmic side. Note=Inner surface of plasma membrane possibly with attachment requiring acylation of the C-terminal cysteine (By similarity with RAS).
Protein Families
Small GTPase superfamily, Ras family
Database Links

HGNC: 10447

OMIM: 165090

KEGG: hsa:6237

STRING: 9606.ENSP00000246792

UniGene: Hs.515536

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