Recombinant Human Serine/threonine-protein kinase/endoribonuclease IRE1(ERN1) ,partial

Code CSB-YP007795HU
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Source Yeast
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Code CSB-EP007795HU
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Source E.coli
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Code CSB-EP007795HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007795HU
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Source Baculovirus
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Code CSB-MP007795HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names ERN1
Uniprot No. O75460
Alternative Names Endoplasmic reticulum (ER) to nucleus signalling 1; Endoplasmic reticulum to nucleus signaling 1; Endoplasmic reticulum-to-nucleus signaling 1; Endoribonuclease; ER to nucleus signaling 1; ERN 1; Ern1; ERN1_HUMAN; hIRE 1p; hIRE1p; Inositol requiring 1; Inositol requiring 1, S. cerevisiae, homolog of; Inositol requiring enzyme 1, S. cerevisiae, homolog of; Inositol requiring protein 1; inositol-requiring enzyme 1; Inositol-requiring protein 1; IRE 1; IRE 1a; IRE 1P; Ire1 alpha; Ire1-alpha; IRE1a; Ire1alpha; IRE1P; MGC163277; MGC163279; Protein kinase/endoribonuclease; RGD1559716; Serine/threonine protein kinase/endoribonuclease IRE1
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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 Q&A
Q:

Please could you let me know if CSB-EP007795HU is phosphorylated or not?

A:
Thanks for your inquiry. This protein is expressed in E.coli, which is a prokaryotic expression system, so it's not phosphorylated.

Target Background

Function
(From Uniprot)
Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation
Gene References into Functions
  1. results suggest that apocynin protects ECs against ER stress-induced apoptosis via IRE1alpha involvement. These findings may provide a novel mechanistic explanation for the anti-apoptotic effect of apocynin in ER stress. PMID: 29696609
  2. Study results provide evidence that DDRGK1 is essential for endoplasmic reticulum (ER) homeostasis regulation in both human cancer cells and mouse hematopoietic stem cells. Depletion of DDRGK1 activates apoptotic pathway by targeting the ER-stress sensor IRE1alpha. DDRGK1 regulates IRE1alpha protein stability via its interaction with the kinase domain of IRE1alpha. PMID: 28128204
  3. IRE1 was up-regulated during excisional wound healing at the time in wound healing consistent with that of the proliferative phase. Inhibition of IRE1 led to decreased scar formation. PMID: 29316036
  4. urinary levels of the spliced X-box binding protein 1 (sXBP1) mRNA as a proxy of inositol-requiring enzyme 1alpha (IRE1alpha) activity because sXBP1 is absolutely sensitive and specific for endoplasmic reticulum stress. PMID: 29276149
  5. Here, the authors show that similar to yeast, human IRE1alpha's endoplasmic reticulum-lumenal domain (hIRE1alpha LD) binds peptides with a characteristic amino acid bias. Peptides and unfolded proteins bind to hIRE1alpha LD's MHC-like groove and induce allosteric changes that lead to its oligomerization. PMID: 28971800
  6. Three branches of the Unfolded Protein Response (UPR) have been described, including the activation of the inositol-requiring enzyme 1 (IRE1), the pancreatic ER kinase (PKR)-like ER kinase (PERK), and the activating transcription factor 6 (ATF6). PMID: 28105371
  7. IRE1alpha is expressed at lower levels in higher-grade gliomas, suggesting greater antitumor efficacy of the oncolytic virus M1. Taken together, these findings illustrate a defensive mechanism of glioma cells against the oncolytic virus M1 and identify possible approaches to enhance the oncolytic viral protein accumulation and the subsequent lysis of tumor cells. PMID: 29263275
  8. Systematic mutation of the AREs (ARE1-3) in the LDLR 3'UTR and expression of each mutant coupled to a luciferase reporter in Huh7 cells demonstrated that ARE1 is required for rapid LDLR mRNA decay and 5-AzaC-induced mRNA stabilization via the IRE1alpha-EGFR-ERK1/2 signaling cascade. PMID: 29208426
  9. Pharmacologic modulation of IRE1 counteracts metaflammation and alleviates atherosclerosis. PMID: 28137856
  10. Present study demonstrates that fine-tuning of the expression of proliferation related transcription factor genes depends upon glucose and glutamine deprivation in IRE1-dependent manner and possibly contributes to slower tumor growth after inhibition of IRE1. PMID: 29537195
  11. The activation of IRE1alpha by the hepatitis C virus protein NS4B in XBP1-proficient cells conferred apoptosis resistance and promoted viral replication. PMID: 28588082
  12. this study highlights an important role for Nck1 in fine-tuning IRE1alpha expression and signaling that regulate PTP1B expression and subsequent activation of the PI3K-Akt pathway in HepG2 cells. PMID: 28455143
  13. IRE1alpha-XBP1 pathway regulates Mel-RMu cell proliferation and progression by activating IL-6/STAT3 signaling. PMID: 28222747
  14. The authors propose that the Sec61-IRE1alpha complex defines the extent of IRE1alpha activity and may determine cell fate decisions during endoplasmic reticulum stress conditions. PMID: 28504640
  15. ERN1 and ALPK1 inhibit differentiation of bi-potential tumor-initiating cells in human triple negative breast cancer. PMID: 27829216
  16. IRE1 deficiency fully restored the learning and memory capacity of AD mice, associated with improved synaptic function and improved long-term potentiation (LTP). At the molecular level, IRE1 deletion reduced the expression of amyloid precursor protein (APP) in cortical and hippocampal areas of AD mice. PMID: 28341998
  17. The biologic processes altered by aberrant IRE1alpha-XBP1 signaling in these innate immune cells. PMID: 26979393
  18. Inhibition of IRE1 modifies the hypoxic regulation of GADD34 family gene expression in cultured glioma cells. PMID: 29227599
  19. Changes in the expression level of nuclear genes encoding mitochondrial proteins possibly reflect metabolic reprogramming of mitochondria by hypoxia and IRE1-mediated endoplasmic reticulum stress signaling and correlate with suppression of glioma cell proliferation under inhibition of the IRE1 enzyme function. PMID: 29235326
  20. The inhibition of IRE1 changes sensitivity of the expression of down-stream genes to glutamine deprivation in cultured glial tumor cells. PMID: 29235329
  21. The present study demonstrated that the inhibition of IRE1 in glioma cells affected the hypoxic regulation of the expression of down-stream genes in various directions, though hypoxic conditions did not abolish the effect of IRE1 inhibition on the expression of respective genes. PMID: 29235836
  22. Inhibition of IRE1 modifies hypoxic regulation of pentose-phosphate pathway genes expression in cultured glioma cells. PMID: 29236388
  23. Study reports that the endoplasmic reticulum luminal co-chaperone ERdj4/DNAJB9 is a selective IRE1 repressor that promotes a complex between the luminal Hsp70 BiP and the luminal stress-sensing domain of IRE1alpha. PMID: 29198525
  24. The present study is the first to uncover a key prosurvival modulator, Yip1A, which coordinates IRE1 signaling with PERK signaling to support the survival of HeLa and CaSki cervical cancer cells. PMID: 28358375
  25. Inositol-requiring kinase 1 may be a useful biomarker to predict recurrence in surgically resected lung adenocarcinoma patients. PMID: 28334878
  26. Fortilin directly interacts with the cytoplasmic domain of IRE1alpha, inhibits both kinase and endoribonuclease (RNase) activities of this stress sensor, and protects cells against apoptotic cell death at both cellular and whole animal levels. PMID: 28550308
  27. Overall, these data demonstrate that hypoxia can suppress adiponectin expression and activate the PERK and IRE1 signaling pathways in differentiated adipocytes, and this two pathways are involved in the suppression of adiponectin expression induced by hypoxia. PMID: 28888981
  28. ER stress-regulated IRE1 dependent decay is involved in regulation of hepatic diseases. (review) PMID: 27774654
  29. The unfolded protein response reduces glucose metabolism via IRE1 signaling. PMID: 28093214
  30. Results of this investigation demonstrate that inhibition of IRE1 signaling enzyme function affects the expression of NRIP1, EBBP, ESRRA, E2IG5, PGRMC2, and SLC39A6 genes in U87 glioma cells in gene specific manner and these changes possibly contribute to the suppression of the cell proliferation. Most of these genes are regulated by hypoxia and preferentially through IRE1 signaling pathway of endoplasmic reticulum stress PMID: 28222026
  31. IRE1alpha was shown to cleave miR-150 and thereby to release the suppressive effect that miR-150 exerted on alphaSMA expression through c-Myb. Inhibition of IRE1alpha was also demonstrated to block endoplasmic reticulum expansion through an XBP-1-dependent pathway. PMID: 27226027
  32. IRE-1 has an ancient function as a cytoplasmic sentinel that activates p38 and SKN-1(Nrf2). csteine modifications induced by ROS signals can direct proteins to adopt unexpected functions and may coordinate many cellular processes. PMID: 27540856
  33. The findings indicate that IRE1-XBP1 downregulation distinguishes germinal center B-cell-like diffuse large B-cell lymphoma (DLBCL) from other DLBCL subtypes and contributes to tumor growth. PMID: 28167662
  34. Western blot analysis of subcutaneously implanted AsPC-1 and BxPC-3 tumors as well as orthotopically implanted Panc-1 tumors demonstrated upregulation of BIP, CHOP, and IRE1alpha expression in the tumor lysates from penfluridol-treated mice as compared to tumors from control mice PMID: 28618969
  35. the ABL family of tyrosine kinases rheostatically enhances IRE1alpha's enzymatic activities, thereby potentiating endoplasmic reticulum stress-induced apoptosis. PMID: 28380378
  36. Pre-ischemia melatonin treatment alleviated acute neuronal injury after ischemic stroke by inhibiting endoplasmic reticulum stress-dependent autophagy via PERK and IRE1 signalings PMID: 28178380
  37. IRE1 is involved in multivesicular body information during endoplasmic reticulum stress. PMID: 27725157
  38. Comparison of this structure with other existing structures of IRE1alpha and integration of our extensive structure activity relationship (SAR) data has led us to formulate a model to rationalize how ATP-binding site ligands are able to control the IRE1alpha oligomeric state and subsequent RNase domain activity PMID: 27227314
  39. Our data indicate that reduced response of IRE1alpha/Xbp-1 signaling pathway to bortezomib may contribute to drug resistance in myeloma cells PMID: 27647225
  40. heat stress simultaneously activates both the unfolded protein response (UPR) and autophagy, followed by the activation of a negative feedback system in UPR by modulating the responses related to the IRE1alpha-XBP-1 axis. PMID: 27743894
  41. these findings underscore the essential role of cytosine nucleotide at +1 in the 3' splice site for determining cleavage specificity of hIRE1alpha. PMID: 28027394
  42. Crucially, Chlamydia trachomatis infection resulted in robust IRE1alpha RNAse activity that was dependent on TLR4 signalling and inhibition of IRE1alpha RNAse activity prevented PKR activation. PMID: 27021640
  43. Pretreatment by IRE1 agonist tunicamycin or JNK agonist anisomycin attenuated the effect of psoralen on osteoporotic osteoblasts. Psoralen inhibited apoptosis of osteoporotic osteoblasts by regulating IRE1-ASK1-JNK pathway PMID: 28349059
  44. Results indicate that excessive activation of the endoplasmic reticulum stress-associated IRE1alpha pathway is involved in LC neuronal apoptosis induced by single prolonged stress exposure; this may be a crucial mechanism of the pathogenesis of post- traumatic stress disorder PMID: 27059130
  45. these results confirmed that ER stress-mediated apoptosis contributes to the protection effects of naringenin against H/R injury, which is potentially involved in ATF6, IRE1alpha and PERK signaling activation. PMID: 27785700
  46. Structural and mechanistic studies of IRE1 PMID: 27686654
  47. Inhibition of IRE1 signaling enzyme function affects the expression of NR3C1 and related genes in U87 glioma cells in gene specific manner and all these genes are regulated by hypoxia preferentially through IRE1 signaling pathway of the endoplasmic reticulum stress. PMID: 27560795
  48. Data suggest that ubiquitin D (UBD) provides a negative feedback on cytokine-induced activation of the endoplasmic reticulum to nucleus signaling 1 (IRE1alpha)/c-Jun N-terminal kinase (JNK) pro-apoptotic pathway in cytokine-exposed beta cells. PMID: 27044747
  49. we used KO hepatocytes to demonstrate that PA-induced EV release was mediated by inositol requiring enzyme 1alpha (IRE1alpha)/X-box binding protein-1 PMID: 26621917
  50. Using drugs that specifically inhibit or activate the PERK or IRE1alpha sensors, we demonstrate that signalling through the PERK axis activates this expression, through a transcriptional mechanism PMID: 26634309

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Subcellular Location Endoplasmic reticulum membrane, Single-pass type I membrane protein
Protein Families Protein kinase superfamily, Ser/Thr protein kinase family
Tissue Specificity Ubiquitously expressed. High levels observed in pancreatic tissue.
Database Links

HGNC: 3449

OMIM: 604033

KEGG: hsa:2081

STRING: 9606.ENSP00000401445

UniGene: Hs.133982

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