ERN1 Research Reagents

Serine/threonine-protein kinase/endoribonuclease IRE1 is a protein in humans that is encoded by ERN1 gene. Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation.

The following ERN1 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

ERN1 Antibodies

ERN1 Antibodies for Homo sapiens (Human)

ERN1 Proteins

ERN1 Proteins for Homo sapiens (Human)

ERN1 Proteins for Mus musculus (Mouse)

ERN1 Background

Endoplasmic reticulum (ER) to nucleus signaling 1 protein, referred to as ERN1, is a human homologue of the yeast IRE1 ((inositol-requiring enzyme-1), so it is also called human IRE1α. ERN1 contains a cytoplasmic domain that is highly conserved to the yeast counterpart having a Ser/Thr protein kinase domain and a domain homologous to RNase L. ERN1/IRE1α is a special transmembrane sensor protein indispensable for the ER unfolded protein response (UPR). In unstressed cells, the ER luminal domain retains inactive as a monomer by binding to the ER chaperone HSPA5/BiP. Misfolded proteins build up in the ER, causing the release of HSPA5/BiP, which allows the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity. Activation of ERN1 by the association of its N-terminal ER luminal domains promotes autophosphorylation by its cytoplasmic kinase domain, leading to activation of the C-terminal ribonuclease domain, which splices Xbp1 mRNA generating an active Xbp1s transcriptional activator [1].

[1] Ali MM1, Bagratuni T, et al. Structure of the Ire1 autophosphorylation complex and implications for the unfolded protein response [J]. EMBO J. 2011 Mar 2;30(5):894-905.

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