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Phospho-ERN1 (S724) recombinant monoclonal antibody was prepared by cloning the coding sequence for the phospho-ERN1 (S724) monoclonal antibody (produced by immunizing animals with the synthetic phosphopeptide of ERN1) into the plasmids and transfecting the clones into cell lines. It is a rabbit IgG purified through the affinity-chromatography method. This phospho-ERN1 (S724) antibody detects endogenous levels of human ERN1 only when phosphorylated at Ser724. It can be applied in ELISA, WB, and IF analyses.
Phosphorylation at Ser724 residue of the ERN1 protein can regulate its transcriptional and enzymatic activities. Phospho-ERN1 (S724) antibodies are used to analyze ERN1 Ser724 phosphorylation, which is a highly conserved site within the kinase activation domain.
Applications : Immunoblot analysis
Sample type: cell
Review: Immunoblot analysis of IRE1α in lysates of Kupffer cells and hepatocytes freshly isolated from livers of male MΦKO mice or Ern1-flox/flox control littermates. HSP90 was used as the loading control. B–E, MΦKO or floxed control mice were subjected to sham or hepatic warm ischemia/reperfusion (n=3–4 per group).