Phospho-ERN1 (S724) Antibody

Code CSB-RA007795A724phHU
Size US$350
Uniprot No. O75460
  • Western Blot
    Positive WB detected in: 293 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate (treated with Calyculin A or EGF)
    All lanes: Phospho-ERN1 antibody at 0.75μg/ml
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 110 kDa
    Observed band size: 110 kDa

  • Immunofluorescence staining of Hela cells with CSB-RA007795A724phHU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunogen A synthesized peptide derived from Human Phospho-ERN1 (S724)
Species Reactivity Human
Tested Applications ELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200
Relevance Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation (PubMed:9637683, PubMed:11175748, PubMed:12637535). The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis (PubMed:24508390).
Form Liquid
Conjugate Non-conjugated
Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
Alias Serine/threonine-protein kinase/endoribonuclease IRE1, Endoplasmic reticulum-to-nucleus signaling 1, hIRE1p, Ire1-alpha, IRE1a, Serine/threonine-protein kinase, Endoribonuclease, ERN1
Immunogen Species Human
Research Area Signal Transduction
Gene Names ERN1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation
Subcellular Location Endoplasmic reticulum membrane, Single-pass type I membrane protein
Protein Families Protein kinase superfamily, Ser/Thr protein kinase family
Tissue Specificity Ubiquitously expressed. High levels observed in pancreatic tissue.
Database Links

HGNC: 3449

OMIM: 604033

KEGG: hsa:2081

STRING: 9606.ENSP00000401445

UniGene: Hs.133982

Pathway Apoptosis
Protein processing in endoplasmic reticulum
ER Stress pathway

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