Name: Recombinant Human Transcriptional enhancer factor TEF-1(TEAD1)
Brand: CUSABIO
SKU: CSB-YP023363HU

Product Details

Description	Recombinant Human Transcriptional enhancer factor TEF-1 (TEAD1) is a full-length protein expressed in the yeast. It was fused with a 6xHis-tag at the N-terminus. This TEAD1 protein containing 426 amino acids was identified and validated by the LC-MS/MS Analysis. Its purity is greater than 90% as determined by SDS-PAGE. The purified TEAD1 migrated on the SDS-PAGE at a molecular mass of about 50 kDa. Because this protein is in stock, the delivery time will be relatively shortened. This recombinant TEAD1 protein may be used for antibody production and in the studies of the cardiovascular research field. TEAD-1 is required for cardiac morphogenesis, and its relatives cannot compensate for the TEAD-1 function during embryogenesis. And TEAD-1 possesses transactivation activity on the TGF-α promoter through its specific interaction with the proximal regulatory element (PRE). This may suggest a strategy for controlling the function of TGF-α, which is frequently expressed in various cancers and is involved in the genesis and maintenance of these malignancies.
Purity	Greater than 90% as determined by SDS-PAGE.
Target Names	TEAD1
Uniprot No.	P28347
Research Area	Cardiovascular
Alternative Names	AA; Atrophia areata peripapillary chorioretinal degeneration; NTEF 1; NTEF-1; NTEF1; Protein GT IIC; Protein GT-IIC; REF 1; REF1; SV40 transcriptional enhancer factor; TCF 13; TCF-13; TCF13; TEA domain family member; TEA domain family member 1 (SV40 transcriptional enhancer factor); TEA domain family member 1; TEAD 1; TEAD 1 protein; TEAD-1; TEAD1; TEAD1 protein; TEAD1_HUMAN; TEF 1; TEF1; Transcription factor 13 (SV40 transcriptional enhancer factor); Transcription factor 13; Transcriptional enhancer factor 1; Transcriptional enhancer factor TEF-1; Transcriptional enhancer factor TEF1
Species	Homo sapiens (Human)
Source	Yeast
Expression Region	1-426aa
Target Protein Sequence	MEPSSWSGSESPAENMERMSDSADKPIDNDAEGVWSPDIEQSFQEALAIYPPCGRRKIILSDEGKMYGRNELIARYIKLRTGKTRTRKQVSSHIQVLARRKSRDFHSKLKDQTAKDKALQHMAAMSSAQIVSATAIHNKLGLPGIPRPTFPGAPGFWPGMIQTGQPGSSQDVKPFVQQAYPIQPAVTAPIPGFEPASAPAPSVPAWQGRSIGTTKLRLVEFSAFLEQQRDPDSYNKHLFVHIGHANHSYSDPLLESVDIRQIYDKFPEKKGGLKELFGKGPQNAFFLVKFWADLNCNIQDDAGAFYGVTSQYESSENMTVTCSTKVCSFGKQVVEKVETEYARFENGRFVYRINRSPMCEYMINFIHKLKHLPEKYMMNSVLENFTILLVVTNRDTQETLLCMACVFEVSNSEHGAQHHIYRLVKD Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight	49.9kDa
Protein Length	Full Length
Tag Info	N-terminal 6xHis-tagged
Form	Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer	If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Note: If you have any special requirement for the glycerol content, please remark when you place the order. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs	Protein FAQs
Storage Condition	Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time	3-7 business days
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA	Please contact us to get it.

Customer Reviews and Q&A

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■ Q&A

I have a question about TEAD1 (CSB-YP023363HU).
Is it possible to request this product in PBS instead of the Tris-based buffer it is normally supplied in?

Thanks for your inquiry，
We could use PBS as storage buffer. Please kindly remark this requirement when send your PO. We need to tell you in advance that we're not sure whether this protein can mantain stable in PBS. If this protein is unstable in this buffer, we will deliver this protein in default buffer (Tris buffer with 50% glycerol).

Is there an option to elute the protein into water and then lyophilize it, so that there is no salt in the protein? Additionally, would there be an additional charge?

Thanks for your inquiry. For CSB-YP023363HU, we don't suggest to elute the protein into water and then lyophilize it, because the protein will be unstable if there is no suitable PH and salt ion concentration.
For the "salt" you mentioned, if you refer to Nacl, we could guarantee no salt in the protein. We could use tris-based buffer or HEPES. Please kindly confirm with the customer which buffer they want. As long as the protein is stable, we could replace any buffer that customer specifies.
You only need to remark this requirement before placing your order. There is no extra cost.

Target Background

Function	Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif.
Gene References into Functions	YAP1 interacted with TEAD1, exerted their transcriptional control of the functional target, glucose transporter 1 (Glut1). PMID: 28892790 Yap1 post-translational modifications favoring its ubiquitination and apoptosis characterize hepatocellular carcinoma (HCC) with better prognosis, whereas conditions favoring the formation of YAP1-TEAD complexes are associated with aggressiveness and acquisition of stemness features by HCC cells PMID: 27359056 TEAD1 and TEAD4 are oncogenic factors, whose aberrant activation are, in part, mediated by the silence of miR-377-3p, miR-1343-3p and miR-4269. PMID: 28759040 adult human and mouse hearts had more Taz than Yap1 by mRNA and protein expression and their increases in diseased hearts were proportional and did not change Yap1/Taz ratio. Yap1, Taz, and Tead1 were accumulated in the nuclear fraction and cardiomyocyte nuclei of diseased hearts PMID: 29154888 Here, the authors show that TEAD1-expressing skeletal muscle of transgenic mice features a dramatic hyperplasia of muscle stem cells (i.e. satellite cells, SCs) but surprisingly without affecting muscle tissue size. PMID: 27725085 This identifies the YAP1/TEAD1 complex as the representative dysregulated profile of Hippo signaling in OS and provides proof-of-principle that targeting TEAD1 may be a therapeutic strategy of osteosarcoma. PMID: 28483529 The authors show MRTF family proteins bind YAP via a conserved PPXY motif that interacts with the YAP WW domain. This interaction allows MRTF to recruit NcoA3 to the TEAD-YAP transcriptional complex and potentiate its transcriptional activity. PMID: 28028053 MYC and TEAD activity is able to stratify different breast cancer subtypes in large panels of breast cancer patients. PMID: 27433809 Collectively, these results indicate that human papillomavirus 16 E6 induces upregulation of APOBEC3B through increased levels of TEADs, highlighting the importance of the TEAD-APOBEC3B axis in carcinogenesis. PMID: 28077648 Upregulation of transcriptional enhancer activator domain 1 was found in hepatocellular carcinoma tissues and inversely correlated with miR-590-3p. Our results indicate a tumor suppressor role of miR-590-3p in hepatocellular carcinoma through targeting transcriptional enhancer activator domain 1 and suggest its use in the diagnosis and prognosis of liver cancer. PMID: 28349829 TEAD1 mediates YAP1 chromatin-binding genome-wide. PMID: 26295846 show that the proangiogenic microfibrillar-associated protein 5 (MFAP5) is a direct transcriptional target of YAP/TEAD in cholangiocarcinoma cells transcription factors. PMID: 26173433 TAZ negatively regulate transcription of DeltaNp63 through TEAD1,2,3 and 4 transcription factors. PMID: 25995450 Our data suggest that AIC is a genetically heterogeneous disease and is not restricted to the X chromosome, and that TEAD1 mutations may be present in male patients. PMID: 26091538 Our findings suggest that genetic variants of Hippo pathway genes, particularly YAP1 rs11225163, TEAD1 rs7944031 and TEAD4 rs1990330, may independently or jointly modulate survival of CM patients. PMID: 25628125 Suggest central role for TEAD and YAP as signal-responsive regulators of multipotent pancreatic progenitors. PMID: 25915126 the YAP-TEAD interaction can be disrupted using cyclic YAP-like peptides, which targets the HIPPO pathway PMID: 25384421 the first evidence demonstrating that TEAD1 is a novel general repressor of smooth muscle-specific gene expression through interfering with myocardin binding to SRF. PMID: 24344135 identified an intronic region of the NAIP gene responding to TEAD1/YAP activity, suggesting that regulation of NAIP by TEAD1/YAP is at the transcriptional level PMID: 23994529 Data indicate that knockdown of TEAD1/3/4 induces an almost identical cellular senescent phenotype as YAP silencing. PMID: 23576552 our data reveal a new, Livin-dependent, apoptotic role for TEAD1 in mammals and provide mechanistic insight downstream of TEAD1 deregulation in cancers. PMID: 23029054 TEAD1 has non-AUG translation initiation PMID: 1851669 These results are consistent with two plausible models of cryptic MCAT enhancer regulation by Pur alpha, Pur beta, and MSY1 involving either competitive single-stranded DNA binding or masking of MCAT-bound transcription enhancer factor-1. PMID: 11751932 Transcription enhancer factor 1 binds multiple muscle MEF2 and A/T-rich elements during fast-to-slow skeletal muscle fiber type transitions PMID: 12861002 the mutation in the TEAD1 gene is the cause of Sveinsson's chorioretinal atrophy. PMID: 15016762 regulation of IFITM3 by TEF-1 demonstrates that TEF-1 dependent regulation is more widespread than its previously established role in the expression of muscle specific genes PMID: 18177740 TEAD1 inhibits prolactin gene expression in cultured human uterine decidual cells. PMID: 18775765 TEAD1 and the ubiquitin ligase c-Cbl were identified as novel basal cell markers in prostate cancer PMID: 19002168 Here we show that during vertebrate neural tube development, the TEA domain transcription factor (TEAD) is the cognate DNA-binding partner of YAP. PMID: 19015275 The primary cellular origin of circumpapillary dysgenesis of the pigment epithelium is within the choroid instead of the pigment epithelium. PMID: 19410955 A missense mutation (Y421H) in TEAD1 is tightly linked to Sveinsson's chorioretinal atrophy (SCRA), an autosomal dominant eye disease characterized by symmetrical lesions radiating from the optic disc involving the retina and the choroid. PMID: 15016762 TEAD1 (TEF-1) interacts with a muscle-specific cofactor to promote skeletal muscle gene expression. PMID: 12376544 Show More Hide All
Involvement in disease	Sveinsson chorioretinal atrophy (SCRA)
Subcellular Location	Nucleus.
Tissue Specificity	Preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.
Database Links	HGNC: 11714 OMIM: 108985 KEGG: hsa:7003 STRING: 9606.ENSP00000354588 UniGene: Hs.655331

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Code	CSB-YP023363HU
Size	US$1298
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP023363HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) TEAD1. Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP023363HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) TEAD1.
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Recombinant Human Transcriptional enhancer factor TEF-1(TEAD1)

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