Code | CSB-YP023363HU |
Size | US$1298 |
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Description |
Recombinant Human Transcriptional enhancer factor TEF-1 (TEAD1) is a full-length protein expressed in the yeast. It was fused with a 6xHis-tag at the N-terminus. This TEAD1 protein containing 426 amino acids was identified and validated by the LC-MS/MS Analysis. Its purity is greater than 90% as determined by SDS-PAGE. The purified TEAD1 migrated on the SDS-PAGE at a molecular mass of about 50 kDa. Because this protein is in stock, the delivery time will be relatively shortened. This recombinant TEAD1 protein may be used for antibody production and in the studies of the cardiovascular research field. TEAD-1 is required for cardiac morphogenesis, and its relatives cannot compensate for the TEAD-1 function during embryogenesis. And TEAD-1 possesses transactivation activity on the TGF-α promoter through its specific interaction with the proximal regulatory element (PRE). This may suggest a strategy for controlling the function of TGF-α, which is frequently expressed in various cancers and is involved in the genesis and maintenance of these malignancies. |
Purity | Greater than 90% as determined by SDS-PAGE. |
Target Names | TEAD1 |
Uniprot No. | P28347 |
Research Area | Cardiovascular |
Alternative Names |
AA; Atrophia areata peripapillary chorioretinal degeneration; NTEF 1; NTEF-1; NTEF1; Protein GT IIC; Protein GT-IIC; REF 1; REF1; SV40 transcriptional enhancer factor; TCF 13; TCF-13; TCF13; TEA domain family member; TEA domain family member 1 (SV40 transcriptional enhancer factor); TEA domain family member 1; TEAD 1; TEAD 1 protein; TEAD-1; TEAD1; TEAD1 protein; TEAD1_HUMAN; TEF 1; TEF1; Transcription factor 13 (SV40 transcriptional enhancer factor); Transcription factor 13; Transcriptional enhancer factor 1; Transcriptional enhancer factor TEF-1; Transcriptional enhancer factor TEF1
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Species | Homo sapiens (Human) |
Source | Yeast |
Expression Region | 1-426aa |
Target Protein Sequence | MEPSSWSGSESPAENMERMSDSADKPIDNDAEGVWSPDIEQSFQEALAIYPPCGRRKIILSDEGKMYGRNELIARYIKLRTGKTRTRKQVSSHIQVLARRKSRDFHSKLKDQTAKDKALQHMAAMSSAQIVSATAIHNKLGLPGIPRPTFPGAPGFWPGMIQTGQPGSSQDVKPFVQQAYPIQPAVTAPIPGFEPASAPAPSVPAWQGRSIGTTKLRLVEFSAFLEQQRDPDSYNKHLFVHIGHANHSYSDPLLESVDIRQIYDKFPEKKGGLKELFGKGPQNAFFLVKFWADLNCNIQDDAGAFYGVTSQYESSENMTVTCSTKVCSFGKQVVEKVETEYARFENGRFVYRINRSPMCEYMINFIHKLKHLPEKYMMNSVLENFTILLVVTNRDTQETLLCMACVFEVSNSEHGAQHHIYRLVKD Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. |
Mol. Weight | 49.9kDa |
Protein Length | Full Length |
Tag Info |
N-terminal 6xHis-tagged |
Form |
Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. |
Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Troubleshooting and FAQs |
Protein FAQs |
Storage Condition | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
Lead Time | 3-7 business days |
Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Datasheet & COA | Please contact us to get it. |
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I have a question about TEAD1 (CSB-YP023363HU).
Is it possible to request this product in PBS instead of the Tris-based buffer it is normally supplied in?
Is there an option to elute the protein into water and then lyophilize it, so that there is no salt in the protein? Additionally, would there be an additional charge?
Function |
Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif.
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Gene References into Functions |
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Involvement in disease | Sveinsson chorioretinal atrophy (SCRA) |
Subcellular Location | Nucleus. |
Tissue Specificity | Preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart. |
Database Links |
HGNC: 11714 OMIM: 108985 KEGG: hsa:7003 STRING: 9606.ENSP00000354588 UniGene: Hs.655331 |