TEAD1, testis-specific transcriptional enhancer 1, alias TEF-1, TEF-2, TEAD4, RTEF-1 and so on. It is a transcription factor, a member of the TEA/ATTS family, encoded by its own gene in human cells.TEAD1 is expressed in a variety of tissues, especially in tissues of the heart, muscle and reproductive system.The mechanism of action of TEAD1 is to regulate the transcription of downstream genes by binding to specific sequences on DNA.TEAD1 can interact with a variety of transcriptional co-activators TEAD1 can interact with a variety of transcriptional co-activators, such as YAP1, to form a complex that activates or inhibits the expression of specific genes.
TEAD1 has a wide range of biological significance, and it is involved in the regulation of a variety of cellular life activities, including cell proliferation, differentiation, migration, and apoptosis, etc. TEAD1 affects the proliferation and cycling process of the cells through its involvement in the regulation of the expression of cell cycle-related genes. In addition, TEAD1 is involved in the development of heart and blood vessels, muscle regeneration, and germ cell differentiation. In tumor cells, the abnormal expression of TEAD1 is closely related to tumor development, and its abnormal function may lead to malignant cell proliferation and metastasis. Therefore, TEAD1 is an important transcription factor in cell biology and disease research, as well as a potential target in drug development and disease treatment.
Recombinant Human Transcriptional enhancer factor TEF-1 (TEAD1), partial (CSB-EP023363HU1)
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
TEAD1 Antibody (CSB-PA023363DSR2HU)
Validated Data
Western Blot
Positive WB detected in: HepG2 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate
All lanes: TEAD1 antibody at 3.6µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
IHC image of CSB-PA023363DSR2HU diluted at 1:441 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of A549 cells with CSB-PA023363DSR2HU at 1:147, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The following TEAD1 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
TEAD1 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA023363GA01HU | TEAD1 Antibody | Human,Mouse,Rat | ELISA,WB |
CSB-PA023363DSR2HU | TEAD1 Antibody | Human | ELISA, WB, IHC, IF |
CSB-PA921314 | TEAD1 Antibody | Human | ELISA,WB |
CSB-PA050232 | TEAD1 Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
TEAD1 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP023363MO CSB-EP023363MO CSB-BP023363MO CSB-MP023363MO CSB-EP023363MO-B |
Recombinant Mouse Transcriptional enhancer factor TEF-1 (Tead1) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
TEAD1 Proteins for Homo sapiens (Human)